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The map label for this gene is pcnB [H]

Identifier: 82702011

GI number: 82702011

Start: 1002118

End: 1003533

Strand: Reverse

Name: pcnB [H]

Synonym: Nmul_A0882

Alternate gene names: 82702011

Gene position: 1003533-1002118 (Counterclockwise)

Preceding gene: 82702012

Following gene: 82702010

Centisome position: 31.52

GC content: 59.89

Gene sequence:

>1416_bases
ATGATTCGTAAACTTATCGATCGCGTCTTCAAGCGCAACACATCCGCTTCCCTCCCTTCTGCCCGCACTTCCTCCACGAA
GCCGCATATCATTACGCGCGACCAGCATGGCATCAGGCGCGATCACATCAGCTCCGGTGCGCTCAAAGTTACCTCAGGCC
TGCAGGAAGCGGGATATTCCGCTTTCATTGTGGGCGGTGCCGCGCGCGACCTGGTGCTGGGATTGGAGCCAAAGGATTTT
GACGTCGCCACGAATGCCACACCGGAGGAAGTACGCGCTGTTTTCCGGCGCTCGCGCATCATTGGCCGCCGTTTCCGGCT
GGTGCATGTCATGTGGGGAGCGGAAACCGTGGAGGTTTCCACCTTCCGCGGCGGCCCATCGGGAGAGACAGGCGGGCTGG
GTGCCGACGAGCATGCGGACGAGCACGGGCGCCTCTTGCGCGATAACGTATTCGGCAGTCAGGAGGAGGATGCGAGACGG
CGGGACTTCACAGTGAATGCGCTGTTCTTCGATCCCGTCCACGAGGAAATCTGGGACTATCTGAACGGCTATGAGGACTT
GAAGGCACGCCGCCTGCGCATCATCGGAGATCCGGTACGGAGATACCGCGAAGATCCCGTACGTATGCTGCGGGTGGTAC
GGCTGGGCGCCAAGCTCGGCATGGAAATCGATCCCGACACGGCCGCTCCTATCAGCGAACTGGCCCCCCTGCTGCTGCAT
GTGCCGCCCTCGCGGCTGTTCGATGAAATGCTGAAATTGCTGCTATCCGGCCATGCGCTGGCCTGCGTGACCGATTTGCA
GAAACGAGGATTGCACCACGGCCTGCTTCCCATGCTGGATGTGATACGGGAACAACCGCTGGGCAAGCGCTTTATCACGC
TCGCGCTCAAAAATACGGATGAGCGCGTGCGGCAGGAAAAGCCTGTATCGCCGGGATTCCTGTTTGCCGCCCTGTTATGG
CACGAGGTGCTCGCATCCTGGAATGCTTACCAGGCGACGGGGGAAAGACCGGTTCCCGCCCTGCACCTGGCAATGAACGA
CGTGCTGGCCGCACAGGACAAGAACCTGGCAATTCCACGCAGGTACGATGCCATCATGAAGGAAATCTGGATGATGCAGC
CGCGCTTTGCCCACCGCTCCGGGAGCAGACCGTTCCGGCTCCTTGAAAATCCCCGCTTTCGCGCTGCCTACGACTTCATG
CTATTACGTTGCGAAAGCGGCGAAGTGGATGAGGAACTGGGCAAATGGTGGGAAGCATTCCAGCATGCAAGTTCCCGCGA
ACGTGAAGCCATGCTGATCAGCGATGAGGGCCAGAAGAGACGCAGGAGAAACCGTGGCCGTAAAAAATCGGGCATTCTCC
CTGCAGGCGAGAGCGACCGCGGAGTATCCGGGAAGCAGGATGGTTCTGCCCCATGA

Upstream 100 bases:

>100_bases
TGGACGGCAGTGGCTGTTTGCCCTCACTTGATCTTCCCCTTTTGGATGAGTTCGCCGCACGGGATGACGGAATCCCGGAG
CTTGTTCATCTGTATTCACA

Downstream 100 bases:

>100_bases
ACGGGGCTGACGACGTTCGAATCGCCTCGCATGCGTATATCGGATTGGGTAGTAATCTGGATGAACCTGTAGCGCATGTG
CAAAAAGCGTTTGAAGAACT

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MIRKLIDRVFKRNTSASLPSARTSSTKPHIITRDQHGIRRDHISSGALKVTSGLQEAGYSAFIVGGAARDLVLGLEPKDF
DVATNATPEEVRAVFRRSRIIGRRFRLVHVMWGAETVEVSTFRGGPSGETGGLGADEHADEHGRLLRDNVFGSQEEDARR
RDFTVNALFFDPVHEEIWDYLNGYEDLKARRLRIIGDPVRRYREDPVRMLRVVRLGAKLGMEIDPDTAAPISELAPLLLH
VPPSRLFDEMLKLLLSGHALACVTDLQKRGLHHGLLPMLDVIREQPLGKRFITLALKNTDERVRQEKPVSPGFLFAALLW
HEVLASWNAYQATGERPVPALHLAMNDVLAAQDKNLAIPRRYDAIMKEIWMMQPRFAHRSGSRPFRLLENPRFRAAYDFM
LLRCESGEVDEELGKWWEAFQHASSREREAMLISDEGQKRRRRNRGRKKSGILPAGESDRGVSGKQDGSAP

Sequences:

>Translated_471_residues
MIRKLIDRVFKRNTSASLPSARTSSTKPHIITRDQHGIRRDHISSGALKVTSGLQEAGYSAFIVGGAARDLVLGLEPKDF
DVATNATPEEVRAVFRRSRIIGRRFRLVHVMWGAETVEVSTFRGGPSGETGGLGADEHADEHGRLLRDNVFGSQEEDARR
RDFTVNALFFDPVHEEIWDYLNGYEDLKARRLRIIGDPVRRYREDPVRMLRVVRLGAKLGMEIDPDTAAPISELAPLLLH
VPPSRLFDEMLKLLLSGHALACVTDLQKRGLHHGLLPMLDVIREQPLGKRFITLALKNTDERVRQEKPVSPGFLFAALLW
HEVLASWNAYQATGERPVPALHLAMNDVLAAQDKNLAIPRRYDAIMKEIWMMQPRFAHRSGSRPFRLLENPRFRAAYDFM
LLRCESGEVDEELGKWWEAFQHASSREREAMLISDEGQKRRRRNRGRKKSGILPAGESDRGVSGKQDGSAP
>Mature_471_residues
MIRKLIDRVFKRNTSASLPSARTSSTKPHIITRDQHGIRRDHISSGALKVTSGLQEAGYSAFIVGGAARDLVLGLEPKDF
DVATNATPEEVRAVFRRSRIIGRRFRLVHVMWGAETVEVSTFRGGPSGETGGLGADEHADEHGRLLRDNVFGSQEEDARR
RDFTVNALFFDPVHEEIWDYLNGYEDLKARRLRIIGDPVRRYREDPVRMLRVVRLGAKLGMEIDPDTAAPISELAPLLLH
VPPSRLFDEMLKLLLSGHALACVTDLQKRGLHHGLLPMLDVIREQPLGKRFITLALKNTDERVRQEKPVSPGFLFAALLW
HEVLASWNAYQATGERPVPALHLAMNDVLAAQDKNLAIPRRYDAIMKEIWMMQPRFAHRSGSRPFRLLENPRFRAAYDFM
LLRCESGEVDEELGKWWEAFQHASSREREAMLISDEGQKRRRRNRGRKKSGILPAGESDRGVSGKQDGSAP

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=227, Percent_Identity=30.8370044052863, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI87081691, Length=411, Percent_Identity=47.4452554744526, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=210, Percent_Identity=29.0476190476191, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360112, Length=276, Percent_Identity=28.6231884057971, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357337, Length=276, Percent_Identity=28.6231884057971, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53263; Mature: 53263

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLIDRVFKRNTSASLPSARTSSTKPHIITRDQHGIRRDHISSGALKVTSGLQEAGYS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHCCCE
AFIVGGAARDLVLGLEPKDFDVATNATPEEVRAVFRRSRIIGRRFRLVHVMWGAETVEVS
EEEECCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEE
TFRGGPSGETGGLGADEHADEHGRLLRDNVFGSQEEDARRRDFTVNALFFDPVHEEIWDY
EECCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCCCHHHHHHCCEEEEEEECHHHHHHHHH
LNGYEDLKARRLRIIGDPVRRYREDPVRMLRVVRLGAKLGMEIDPDTAAPISELAPLLLH
HCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHC
VPPSRLFDEMLKLLLSGHALACVTDLQKRGLHHGLLPMLDVIREQPLGKRFITLALKNTD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCH
ERVRQEKPVSPGFLFAALLWHEVLASWNAYQATGERPVPALHLAMNDVLAAQDKNLAIPR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
RYDAIMKEIWMMQPRFAHRSGSRPFRLLENPRFRAAYDFMLLRCESGEVDEELGKWWEAF
HHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCHHEEEEEEEEEECCCCHHHHHHHHHHHH
QHASSREREAMLISDEGQKRRRRNRGRKKSGILPAGESDRGVSGKQDGSAP
HHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRKLIDRVFKRNTSASLPSARTSSTKPHIITRDQHGIRRDHISSGALKVTSGLQEAGYS
CHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHCCCE
AFIVGGAARDLVLGLEPKDFDVATNATPEEVRAVFRRSRIIGRRFRLVHVMWGAETVEVS
EEEECCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEE
TFRGGPSGETGGLGADEHADEHGRLLRDNVFGSQEEDARRRDFTVNALFFDPVHEEIWDY
EECCCCCCCCCCCCCCCCCCHHCCCHHHHCCCCCCHHHHHHCCEEEEEEECHHHHHHHHH
LNGYEDLKARRLRIIGDPVRRYREDPVRMLRVVRLGAKLGMEIDPDTAAPISELAPLLLH
HCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHC
VPPSRLFDEMLKLLLSGHALACVTDLQKRGLHHGLLPMLDVIREQPLGKRFITLALKNTD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCH
ERVRQEKPVSPGFLFAALLWHEVLASWNAYQATGERPVPALHLAMNDVLAAQDKNLAIPR
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCH
RYDAIMKEIWMMQPRFAHRSGSRPFRLLENPRFRAAYDFMLLRCESGEVDEELGKWWEAF
HHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCHHEEEEEEEEEECCCCHHHHHHHHHHHH
QHASSREREAMLISDEGQKRRRRNRGRKKSGILPAGESDRGVSGKQDGSAP
HHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]