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The map label for this gene is prpC [H]

Identifier: 82701995

GI number: 82701995

Start: 984596

End: 985771

Strand: Direct

Name: prpC [H]

Synonym: Nmul_A0866

Alternate gene names: 82701995

Gene position: 984596-985771 (Clockwise)

Preceding gene: 82701994

Following gene: 82701996

Centisome position: 30.92

GC content: 57.4

Gene sequence:

>1176_bases
ATGAATCTGACAATGAATAAACCTGATGGAAGCACTGTGAGCACTGCGCCGAAGAAAAAATCTGTGGTGTTGTCCGGGGT
GCGGGCTGGCAATACGGCCATTTGCAGCGTGGGGCGTACAGGGAACGATTTGCATTATCGCGGCTATGACATTCTGGATA
TAGCGGAAAGCAGCGAATTTGAAGAAATTGCCTACCTGCTGGTGTACGAAAAGTTGCCCACGGTGGCCGAGCTCGCCGCC
TACAAGGAGAAACTGGAAAAGCTGCGCGATCTGCCGGCCAGGGTAAAAACCCTGCTGGAGACGATACCTGCAAGCGCTCA
TCCCATGGATGTGTTGCGCACAGGAGTTTCCGCGCTGGGTACGGTGCTGCCGGAAGCCGAGATACATACGGTGGAGGGTG
CGCGGGACATCGCAGACCGGCTGCTGGCCTCCATGGGCTCCATGCTGCTGTACTGGTACCACTATTCTCATCACGACCGC
CGCATCGGACTGGACTCAGATGACGATTCCATTGGCGGACATTTTTTGCACCTCCTGCACGGCAAGCCACCCCCCCAATC
CTGGATAAAGGCAATGCATGCCTCCCTGATCCTGTATGCCGAGCATGAGTTCAATGCCTCCACCTTTACCGCTCGAGTGA
TTGCAGGGACGGAATCGGATTTCTACTCCGCCATAACGGGTGCTATCGGCGCGTTGCGCGGCCGCAAACACGGGGGTGCC
AATGAGGTTGCATTTGACATCCAGACACGCTATCGCAGCCCGGATGAGGCCGAGGCGGACATTCGCCGTCGAGTGACGAA
CAAGGAGATCATCACCGGTTTCGGCCATCCCGTCTATACCGCTGGCGACCCGCGCAACCGGGTAATTCGCGAAGTGGCAA
GGAAGCTGTCGGCCGAGGCAGGAGACATGCAGATGTTCAACGTGGCCGAGCGGTTGGAAACGGTGATGTGGAGCATCAAG
AAGATGTTCCCCAACCTGGACTGGTACTCTGCCGTGGGTTATCACATGATGGGCATCCCCACGGCCATGTTCACCCCGCT
GTTTGCGATTGCCCGCACCAGCGGCTGGGCTGCCCACATCATCGAGCAGCGGATGGACGGAAAGATTATTCGCCCTTCGG
CGAATTACATCGGACCGGAAGAACGGGAGTTTGAGCCGATTGAGGCGCGCAGATAG

Upstream 100 bases:

>100_bases
GTATGAATTTCTCGACTACCGTGCCTATGACGAGAAGCTGAATCTTCTGTTCAAGCCCGAAAAACCGGAAGAACCTGAAA
ATGAATGAACCTGAAAATGA

Downstream 100 bases:

>100_bases
CTGCTAGGTAAAGCAGATCAGTGAAGATTTAACTTTATAGATACGCACGACTTCGAAATCCAAAATATTCTTCGCGTAAG
TTGCGGTACTATCCAGAGTC

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNLTMNKPDGSTVSTAPKKKSVVLSGVRAGNTAICSVGRTGNDLHYRGYDILDIAESSEFEEIAYLLVYEKLPTVAELAA
YKEKLEKLRDLPARVKTLLETIPASAHPMDVLRTGVSALGTVLPEAEIHTVEGARDIADRLLASMGSMLLYWYHYSHHDR
RIGLDSDDDSIGGHFLHLLHGKPPPQSWIKAMHASLILYAEHEFNASTFTARVIAGTESDFYSAITGAIGALRGRKHGGA
NEVAFDIQTRYRSPDEAEADIRRRVTNKEIITGFGHPVYTAGDPRNRVIREVARKLSAEAGDMQMFNVAERLETVMWSIK
KMFPNLDWYSAVGYHMMGIPTAMFTPLFAIARTSGWAAHIIEQRMDGKIIRPSANYIGPEEREFEPIEARR

Sequences:

>Translated_391_residues
MNLTMNKPDGSTVSTAPKKKSVVLSGVRAGNTAICSVGRTGNDLHYRGYDILDIAESSEFEEIAYLLVYEKLPTVAELAA
YKEKLEKLRDLPARVKTLLETIPASAHPMDVLRTGVSALGTVLPEAEIHTVEGARDIADRLLASMGSMLLYWYHYSHHDR
RIGLDSDDDSIGGHFLHLLHGKPPPQSWIKAMHASLILYAEHEFNASTFTARVIAGTESDFYSAITGAIGALRGRKHGGA
NEVAFDIQTRYRSPDEAEADIRRRVTNKEIITGFGHPVYTAGDPRNRVIREVARKLSAEAGDMQMFNVAERLETVMWSIK
KMFPNLDWYSAVGYHMMGIPTAMFTPLFAIARTSGWAAHIIEQRMDGKIIRPSANYIGPEEREFEPIEARR
>Mature_391_residues
MNLTMNKPDGSTVSTAPKKKSVVLSGVRAGNTAICSVGRTGNDLHYRGYDILDIAESSEFEEIAYLLVYEKLPTVAELAA
YKEKLEKLRDLPARVKTLLETIPASAHPMDVLRTGVSALGTVLPEAEIHTVEGARDIADRLLASMGSMLLYWYHYSHHDR
RIGLDSDDDSIGGHFLHLLHGKPPPQSWIKAMHASLILYAEHEFNASTFTARVIAGTESDFYSAITGAIGALRGRKHGGA
NEVAFDIQTRYRSPDEAEADIRRRVTNKEIITGFGHPVYTAGDPRNRVIREVARKLSAEAGDMQMFNVAERLETVMWSIK
KMFPNLDWYSAVGYHMMGIPTAMFTPLFAIARTSGWAAHIIEQRMDGKIIRPSANYIGPEEREFEPIEARR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=373, Percent_Identity=72.1179624664879, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1786939, Length=357, Percent_Identity=32.7731092436975, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17555174, Length=368, Percent_Identity=23.0978260869565, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324328, Length=345, Percent_Identity=24.9275362318841, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=402, Percent_Identity=23.134328358209, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640126, Length=340, Percent_Identity=23.8235294117647, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640124, Length=340, Percent_Identity=23.8235294117647, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 43543; Mature: 43543

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTMNKPDGSTVSTAPKKKSVVLSGVRAGNTAICSVGRTGNDLHYRGYDILDIAESSEF
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEECCCEEEECCCCCCH
EEIAYLLVYEKLPTVAELAAYKEKLEKLRDLPARVKTLLETIPASAHPMDVLRTGVSALG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TVLPEAEIHTVEGARDIADRLLASMGSMLLYWYHYSHHDRRIGLDSDDDSIGGHFLHLLH
HHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHC
GKPPPQSWIKAMHASLILYAEHEFNASTFTARVIAGTESDFYSAITGAIGALRGRKHGGA
CCCCHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
NEVAFDIQTRYRSPDEAEADIRRRVTNKEIITGFGHPVYTAGDPRNRVIREVARKLSAEA
CCEEEEEHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCC
GDMQMFNVAERLETVMWSIKKMFPNLDWYSAVGYHMMGIPTAMFTPLFAIARTSGWAAHI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHH
IEQRMDGKIIRPSANYIGPEEREFEPIEARR
HHHHCCCEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLTMNKPDGSTVSTAPKKKSVVLSGVRAGNTAICSVGRTGNDLHYRGYDILDIAESSEF
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEECCCEEEECCCCCCH
EEIAYLLVYEKLPTVAELAAYKEKLEKLRDLPARVKTLLETIPASAHPMDVLRTGVSALG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TVLPEAEIHTVEGARDIADRLLASMGSMLLYWYHYSHHDRRIGLDSDDDSIGGHFLHLLH
HHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHC
GKPPPQSWIKAMHASLILYAEHEFNASTFTARVIAGTESDFYSAITGAIGALRGRKHGGA
CCCCHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
NEVAFDIQTRYRSPDEAEADIRRRVTNKEIITGFGHPVYTAGDPRNRVIREVARKLSAEA
CCEEEEEHHHCCCCCHHHHHHHHHHCCCHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCC
GDMQMFNVAERLETVMWSIKKMFPNLDWYSAVGYHMMGIPTAMFTPLFAIARTSGWAAHI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHH
IEQRMDGKIIRPSANYIGPEEREFEPIEARR
HHHHCCCEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]