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The map label for this gene is prpB [H]

Identifier: 82701994

GI number: 82701994

Start: 983684

End: 984583

Strand: Direct

Name: prpB [H]

Synonym: Nmul_A0865

Alternate gene names: 82701994

Gene position: 983684-984583 (Clockwise)

Preceding gene: 82701993

Following gene: 82701995

Centisome position: 30.89

GC content: 57.11

Gene sequence:

>900_bases
ATGCCTGCCTCTGCCGGTTCCCGTTTTCGCGCAGCGCTTGACGCGGAAATGCCCCTGCAGATCGCAGGCTGCATCAATGC
GTATGCCGCTCGCATGGCAGAGCGTATCGGCTTCAGGGCATTGTATCTCTCGGGTGGAGGGGTGGCTGCGGCATCTCTCG
GAGTACCCGATCTTGCCATTTCCACCCTTGAGGATGTGCTGGTCGACGTGCGGCGCATTACCGATATCACGGATCTGCCG
TTGCTGGTGGACGCGGATACCGGTTTTGGCGGTGTTCTCAATATCGGCCGTACTGTGAAATCATTGATAAAAGCCGGGGC
TGGCGCGCTCCATATCGAAGATCAGGTGCAGGTCAAGCGTTGCGGTCACCTTCCAGGCAAGGCTGTGGTCAGTGAAATGG
AAATGATGGAACGGATTGGCGCTGCGGTGGATGCAAGAATCGATCCCGCTTTCGTCATCATGGCGCGTACGGATGCACTC
GCGGTGGAGGGACTGCAAGCCGCCATTGATCGGGCCTGTGCTTATGTGGAGGCGGGCGCGGACATGATCTTCCCCGAGGC
CGTGGCTGACCTGCAAACCTACCGGCGCTTTGCGGCAGCGGTCAAGGTGCCCATCCTGGCCAACATTACGGAATTCGGCG
CAACTCCCCTCTATACAGTCAAGGAGTTGGCCCAGGCAGATGTTTCCCTGGTGCTTTATCCACTTTCCGCATTTCGCGCC
ATGAGTGCCGCTGCGTTGAATGTATATCGAACAATACGCGCGGAAGGCTCGCAAAAAAGCGCGCTCCCGAGCATGCAGAC
CCGTGCCGAGTTGTATGAATTTCTCGACTACCGTGCCTATGACGAGAAGCTGAATCTTCTGTTCAAGCCCGAAAAACCGG
AAGAACCTGAAAATGAATGA

Upstream 100 bases:

>100_bases
GGATGAAGATGCAGGCGAGCTACCGGGAATTAATGGGAGAGCGCGAGTCGATAAAGCATCTGCTTGGATAAGCCGCACTC
CCGTCTTCGTTTCTCCTTTC

Downstream 100 bases:

>100_bases
ACCTGAAAATGAATGAATCTGACAATGAATAAACCTGATGGAAGCACTGTGAGCACTGCGCCGAAGAAAAAATCTGTGGT
GTTGTCCGGGGTGCGGGCTG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPASAGSRFRAALDAEMPLQIAGCINAYAARMAERIGFRALYLSGGGVAAASLGVPDLAISTLEDVLVDVRRITDITDLP
LLVDADTGFGGVLNIGRTVKSLIKAGAGALHIEDQVQVKRCGHLPGKAVVSEMEMMERIGAAVDARIDPAFVIMARTDAL
AVEGLQAAIDRACAYVEAGADMIFPEAVADLQTYRRFAAAVKVPILANITEFGATPLYTVKELAQADVSLVLYPLSAFRA
MSAAALNVYRTIRAEGSQKSALPSMQTRAELYEFLDYRAYDEKLNLLFKPEKPEEPENE

Sequences:

>Translated_299_residues
MPASAGSRFRAALDAEMPLQIAGCINAYAARMAERIGFRALYLSGGGVAAASLGVPDLAISTLEDVLVDVRRITDITDLP
LLVDADTGFGGVLNIGRTVKSLIKAGAGALHIEDQVQVKRCGHLPGKAVVSEMEMMERIGAAVDARIDPAFVIMARTDAL
AVEGLQAAIDRACAYVEAGADMIFPEAVADLQTYRRFAAAVKVPILANITEFGATPLYTVKELAQADVSLVLYPLSAFRA
MSAAALNVYRTIRAEGSQKSALPSMQTRAELYEFLDYRAYDEKLNLLFKPEKPEEPENE
>Mature_298_residues
PASAGSRFRAALDAEMPLQIAGCINAYAARMAERIGFRALYLSGGGVAAASLGVPDLAISTLEDVLVDVRRITDITDLPL
LVDADTGFGGVLNIGRTVKSLIKAGAGALHIEDQVQVKRCGHLPGKAVVSEMEMMERIGAAVDARIDPAFVIMARTDALA
VEGLQAAIDRACAYVEAGADMIFPEAVADLQTYRRFAAAVKVPILANITEFGATPLYTVKELAQADVSLVLYPLSAFRAM
SAAALNVYRTIRAEGSQKSALPSMQTRAELYEFLDYRAYDEKLNLLFKPEKPEEPENE

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=286, Percent_Identity=67.4825174825175, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790445, Length=259, Percent_Identity=28.957528957529, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32098; Mature: 31967

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASAGSRFRAALDAEMPLQIAGCINAYAARMAERIGFRALYLSGGGVAAASLGVPDLAI
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEHHCCCCHHHH
STLEDVLVDVRRITDITDLPLLVDADTGFGGVLNIGRTVKSLIKAGAGALHIEDQVQVKR
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHH
CGHLPGKAVVSEMEMMERIGAAVDARIDPAFVIMARTDALAVEGLQAAIDRACAYVEAGA
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
DMIFPEAVADLQTYRRFAAAVKVPILANITEFGATPLYTVKELAQADVSLVLYPLSAFRA
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCHHHEEHHHHHHHH
MSAAALNVYRTIRAEGSQKSALPSMQTRAELYEFLDYRAYDEKLNLLFKPEKPEEPENE
HHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCC
>Mature Secondary Structure 
PASAGSRFRAALDAEMPLQIAGCINAYAARMAERIGFRALYLSGGGVAAASLGVPDLAI
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEHHCCCCHHHH
STLEDVLVDVRRITDITDLPLLVDADTGFGGVLNIGRTVKSLIKAGAGALHIEDQVQVKR
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHH
CGHLPGKAVVSEMEMMERIGAAVDARIDPAFVIMARTDALAVEGLQAAIDRACAYVEAGA
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCC
DMIFPEAVADLQTYRRFAAAVKVPILANITEFGATPLYTVKELAQADVSLVLYPLSAFRA
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCHHHEEHHHHHHHH
MSAAALNVYRTIRAEGSQKSALPSMQTRAELYEFLDYRAYDEKLNLLFKPEKPEEPENE
HHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]