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The map label for this gene is cspA [H]

Identifier: 82701971

GI number: 82701971

Start: 953928

End: 954137

Strand: Direct

Name: cspA [H]

Synonym: Nmul_A0840

Alternate gene names: 82701971

Gene position: 953928-954137 (Clockwise)

Preceding gene: 82701970

Following gene: 82701975

Centisome position: 29.96

GC content: 43.33

Gene sequence:

>210_bases
ATGGCAACAGAAACAGGTATTGTGAAATGGTTTAGCGATGATAAAGGCTTTGGCTTTATTACCTCAGATGAAGGTGGAAA
AGATTTGTTTGCCCACTTCTCTTCGATTGTGGGTGAAGGTTTTAAAACGCTGCAAGAGGCCCAGCGTGTCACTTTTGATG
TGGTAAGCGGTCCCAAGGGTTTACAGGCATCGAATATTAGACCTCAGTAA

Upstream 100 bases:

>100_bases
AAGCTGTAAAACGATACCTCTGGTTGACTCTTTCCCTTGATATTCAGCGGCTGATCACTTACAGATGACTGTGAGTGGAA
TTTTATTAAGGAGAAATGAC

Downstream 100 bases:

>100_bases
AAAGTCAGTAAAGCAAACGAAAATCCTGTTGTGGGATTTTCGTTTGAGAAATTGTAACGTCCCCGCGGTTGACCCGCACC
CGCTTCTCAGTCCCCGAACT

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MATETGIVKWFSDDKGFGFITSDEGGKDLFAHFSSIVGEGFKTLQEAQRVTFDVVSGPKGLQASNIRPQ

Sequences:

>Translated_69_residues
MATETGIVKWFSDDKGFGFITSDEGGKDLFAHFSSIVGEGFKTLQEAQRVTFDVVSGPKGLQASNIRPQ
>Mature_68_residues
ATETGIVKWFSDDKGFGFITSDEGGKDLFAHFSSIVGEGFKTLQEAQRVTFDVVSGPKGLQASNIRPQ

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=59.6774193548387, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=54.5454545454545, Blast_Score=78, Evalue=9e-17,
Organism=Escherichia coli, GI1787107, Length=65, Percent_Identity=55.3846153846154, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=56.0606060606061, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=54.8387096774194, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=53.2258064516129, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=54.8387096774194, Blast_Score=73, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7482; Mature: 7351

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATETGIVKWFSDDKGFGFITSDEGGKDLFAHFSSIVGEGFKTLQEAQRVTFDVVSGPKG
CCCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCC
LQASNIRPQ
CCCCCCCCC
>Mature Secondary Structure 
ATETGIVKWFSDDKGFGFITSDEGGKDLFAHFSSIVGEGFKTLQEAQRVTFDVVSGPKG
CCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEHCCCCCCC
LQASNIRPQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10483731 [H]