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The map label for this gene is hisA

Identifier: 82701948

GI number: 82701948

Start: 930104

End: 930877

Strand: Reverse

Name: hisA

Synonym: Nmul_A0815

Alternate gene names: 82701948

Gene position: 930877-930104 (Counterclockwise)

Preceding gene: 82701949

Following gene: 82701947

Centisome position: 29.23

GC content: 54.39

Gene sequence:

>774_bases
ATGCTTATCATTCCCGCTATAGATTTGAAAGACGGTCACTGTGTACGGCTGAAACAGGGTGTGATGGAAAACGTTACTGT
ATTTTCTGAAGATCCTGCTGCCATGGCCAGGCATTGGCTGGATCAAGGCGCCAGAAGACTGCATCTGGTAGACCTGAATG
GCGCGTTTGCGGGCAAACCGAAGAACGAGCTGGCAATCCGCGACATCGTAGATGCCATCGGGGATGAGATTCCGACTCAA
CTCGGCGGCGGCATACGTGATCTGGAAACCATCGAACGTTACCTCGATGACGGTATTACCTACATCATCATCGGCACAGC
CGCAGTAAAAACCCCCGGATTCCTGCACGACGCGTGCAATGCTTTTCCAGGTCATATCATGGTCGGGCTGGATGCAAAGG
ACGGCAAGGTGGCGGTCAATGGCTGGTCCAAGGTGACGGGGCATGACGTGGTGGATCTTGCGAAAAAATTCGAAGACTAT
GGCGTGGAGGCAATCATCTATACCGATATCGGGCGCGATGGCATGTTGAGCGGTGTCAATCTGAAGGCGACCCTGGAGCT
GGCCAGGGCGCTGACGATTCCCGTCATTGCCAGTGGCGGCGTGAGCAGCCTTGATGACGTCAAGGCACTGTGCGAGATGG
AACCGGAAGGTATTGCAGGTGCAATAACGGGACGCGCCATTTATGAAGGGACGCTCGACTTCAAGGTAGCCCAGGAGCTG
GCGGATGAGCTAAGCACCCAATCAAGCCCCAGGGATACGACAATTCTCTTTTGA

Upstream 100 bases:

>100_bases
CTCCTCGGTAACTTTGTCCGCTGGAAGCCCGTTTGAGAAACGTTAGAAGGTTTGCGCGCTACGAATTCTGAAACTCAATT
CATTTTTATCCTGATTCAAA

Downstream 100 bases:

>100_bases
TGAAACATCCCATCTCGTCCAGCCATACAGGCTGGAGCGGTTCCCGACTCGGATTATTCTTTTTTCTATCACTACAACTC
CCTTACGGAAAGCCCGAGTC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLIIPAIDLKDGHCVRLKQGVMENVTVFSEDPAAMARHWLDQGARRLHLVDLNGAFAGKPKNELAIRDIVDAIGDEIPTQ
LGGGIRDLETIERYLDDGITYIIIGTAAVKTPGFLHDACNAFPGHIMVGLDAKDGKVAVNGWSKVTGHDVVDLAKKFEDY
GVEAIIYTDIGRDGMLSGVNLKATLELARALTIPVIASGGVSSLDDVKALCEMEPEGIAGAITGRAIYEGTLDFKVAQEL
ADELSTQSSPRDTTILF

Sequences:

>Translated_257_residues
MLIIPAIDLKDGHCVRLKQGVMENVTVFSEDPAAMARHWLDQGARRLHLVDLNGAFAGKPKNELAIRDIVDAIGDEIPTQ
LGGGIRDLETIERYLDDGITYIIIGTAAVKTPGFLHDACNAFPGHIMVGLDAKDGKVAVNGWSKVTGHDVVDLAKKFEDY
GVEAIIYTDIGRDGMLSGVNLKATLELARALTIPVIASGGVSSLDDVKALCEMEPEGIAGAITGRAIYEGTLDFKVAQEL
ADELSTQSSPRDTTILF
>Mature_257_residues
MLIIPAIDLKDGHCVRLKQGVMENVTVFSEDPAAMARHWLDQGARRLHLVDLNGAFAGKPKNELAIRDIVDAIGDEIPTQ
LGGGIRDLETIERYLDDGITYIIIGTAAVKTPGFLHDACNAFPGHIMVGLDAKDGKVAVNGWSKVTGHDVVDLAKKFEDY
GVEAIIYTDIGRDGMLSGVNLKATLELARALTIPVIASGGVSSLDDVKALCEMEPEGIAGAITGRAIYEGTLDFKVAQEL
ADELSTQSSPRDTTILF

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=34.156378600823, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1788336, Length=214, Percent_Identity=25.7009345794392, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_NITMU (Q2YAU9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411514.1
- ProteinModelPortal:   Q2YAU9
- SMR:   Q2YAU9
- STRING:   Q2YAU9
- GeneID:   3785859
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0815
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q2YAU9
- ProtClustDB:   PRK00748
- BioCyc:   NMUL323848:NMUL_A0815-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27514; Mature: 27514

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIPAIDLKDGHCVRLKQGVMENVTVFSEDPAAMARHWLDQGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
KNELAIRDIVDAIGDEIPTQLGGGIRDLETIERYLDDGITYIIIGTAAVKTPGFLHDACN
CCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHC
AFPGHIMVGLDAKDGKVAVNGWSKVTGHDVVDLAKKFEDYGVEAIIYTDIGRDGMLSGVN
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
LKATLELARALTIPVIASGGVSSLDDVKALCEMEPEGIAGAITGRAIYEGTLDFKVAQEL
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHH
ADELSTQSSPRDTTILF
HHHHHCCCCCCCCEEEC
>Mature Secondary Structure
MLIIPAIDLKDGHCVRLKQGVMENVTVFSEDPAAMARHWLDQGARRLHLVDLNGAFAGKP
CEEEEEEECCCCCCHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
KNELAIRDIVDAIGDEIPTQLGGGIRDLETIERYLDDGITYIIIGTAAVKTPGFLHDACN
CCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHC
AFPGHIMVGLDAKDGKVAVNGWSKVTGHDVVDLAKKFEDYGVEAIIYTDIGRDGMLSGVN
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
LKATLELARALTIPVIASGGVSSLDDVKALCEMEPEGIAGAITGRAIYEGTLDFKVAQEL
HHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHH
ADELSTQSSPRDTTILF
HHHHHCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA