Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 82701949

GI number: 82701949

Start: 930942

End: 931580

Strand: Reverse

Name: hisH

Synonym: Nmul_A0816

Alternate gene names: 82701949

Gene position: 931580-930942 (Counterclockwise)

Preceding gene: 82701950

Following gene: 82701948

Centisome position: 29.26

GC content: 56.65

Gene sequence:

>639_bases
ATGACTGATATTGCAATTGTTGACTACGGCATGGGAAACCTGCGTTCCGTAGCCAAAGCACTGGAACATGTCGCCCCCGA
GGCTGTCATCGCTGTCACCAATAACCCTGAAGTAGTGCGGAAAGCGGAGCGCGTGGTCGTTCCCGGGCAGGGCGCCATGC
CGGACTGCCTGCGTGAACTGGACCGGCTCGGATTGCGCGAAGCCGTGCGGGACGCAGCCGCCAACAAACCATTCCTGGGT
ATCTGCATCGGCCTGCAAATGCTGTTCGACAGCAGCGAGGAGGGCAATGTCTCCGGGCTCGGTATCGTGCCGGGAAGGGT
AAAGCGTTTTCCCGCAAGTGCGATGAAAGATGAAAAAGGGCAGAAACTCAAGGTGCCGCACATGGGTTGGAATCAGGTCC
ATCAGTCGGTCGGGCATTCCCTCTGGAAAAACATTGCCAATGATTCGCGCTTTTATTTCGTGCACAGCTACTATGTCGAA
CCCGCTGACGCCGATTCGGCGGGTCACAGTGCTTACCCCTTTTCTTTTACTTGTGCGGTGGCGAAAGATAACATTTTTGC
CGTACAGTTCCACCCCGAAAAGAGCCACGCGGCCGGCCTGACGCTCCTCGGTAACTTTGTCCGCTGGAAGCCCGTTTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCTGCGCATGGCGGTGGAAGCCGATCCGCGGGCAGCGGGTGCAATACCCTCCACCAAAGGCACTCTGTAATTT
CCCACACTCATAGATGCGGT

Downstream 100 bases:

>100_bases
GAAACGTTAGAAGGTTTGCGCGCTACGAATTCTGAAACTCAATTCATTTTTATCCTGATTCAAAATGCTTATCATTCCCG
CTATAGATTTGAAAGACGGT

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTDIAIVDYGMGNLRSVAKALEHVAPEAVIAVTNNPEVVRKAERVVVPGQGAMPDCLRELDRLGLREAVRDAAANKPFLG
ICIGLQMLFDSSEEGNVSGLGIVPGRVKRFPASAMKDEKGQKLKVPHMGWNQVHQSVGHSLWKNIANDSRFYFVHSYYVE
PADADSAGHSAYPFSFTCAVAKDNIFAVQFHPEKSHAAGLTLLGNFVRWKPV

Sequences:

>Translated_212_residues
MTDIAIVDYGMGNLRSVAKALEHVAPEAVIAVTNNPEVVRKAERVVVPGQGAMPDCLRELDRLGLREAVRDAAANKPFLG
ICIGLQMLFDSSEEGNVSGLGIVPGRVKRFPASAMKDEKGQKLKVPHMGWNQVHQSVGHSLWKNIANDSRFYFVHSYYVE
PADADSAGHSAYPFSFTCAVAKDNIFAVQFHPEKSHAAGLTLLGNFVRWKPV
>Mature_211_residues
TDIAIVDYGMGNLRSVAKALEHVAPEAVIAVTNNPEVVRKAERVVVPGQGAMPDCLRELDRLGLREAVRDAAANKPFLGI
CIGLQMLFDSSEEGNVSGLGIVPGRVKRFPASAMKDEKGQKLKVPHMGWNQVHQSVGHSLWKNIANDSRFYFVHSYYVEP
ADADSAGHSAYPFSFTCAVAKDNIFAVQFHPEKSHAAGLTLLGNFVRWKPV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=38.1642512077295, Blast_Score=127, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.0810810810811, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_NITMU (Q2YAU8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411515.1
- ProteinModelPortal:   Q2YAU8
- SMR:   Q2YAU8
- STRING:   Q2YAU8
- GeneID:   3785860
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0816
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q2YAU8
- BioCyc:   NMUL323848:NMUL_A0816-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23171; Mature: 23040

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 191-191 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIAIVDYGMGNLRSVAKALEHVAPEAVIAVTNNPEVVRKAERVVVPGQGAMPDCLREL
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEECCCCCCHHHHHHH
DRLGLREAVRDAAANKPFLGICIGLQMLFDSSEEGNVSGLGIVPGRVKRFPASAMKDEKG
HHCCHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCHHHCCCCC
QKLKVPHMGWNQVHQSVGHSLWKNIANDSRFYFVHSYYVEPADADSAGHSAYPFSFTCAV
CEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
AKDNIFAVQFHPEKSHAAGLTLLGNFVRWKPV
ECCCEEEEEECCCCCHHHHHHHHHCCEEECCC
>Mature Secondary Structure 
TDIAIVDYGMGNLRSVAKALEHVAPEAVIAVTNNPEVVRKAERVVVPGQGAMPDCLREL
CCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEECCCCCCHHHHHHH
DRLGLREAVRDAAANKPFLGICIGLQMLFDSSEEGNVSGLGIVPGRVKRFPASAMKDEKG
HHCCHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCCHHHCCCCC
QKLKVPHMGWNQVHQSVGHSLWKNIANDSRFYFVHSYYVEPADADSAGHSAYPFSFTCAV
CEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
AKDNIFAVQFHPEKSHAAGLTLLGNFVRWKPV
ECCCEEEEEECCCCCHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA