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The map label for this gene is hisF

Identifier: 82701947

GI number: 82701947

Start: 929112

End: 929870

Strand: Reverse

Name: hisF

Synonym: Nmul_A0814

Alternate gene names: 82701947

Gene position: 929870-929112 (Counterclockwise)

Preceding gene: 82701948

Following gene: 82701946

Centisome position: 29.2

GC content: 54.02

Gene sequence:

>759_bases
ATGGGTCTTGCCAAGCGTATCATTCCCTGTCTTGACGTTACGAACGGACGTGTTGTCAAGGGAGTCAAATTTGTCTCACT
TCGAGATGCGGGAGATCCCATCGAAATAGCGCGCCGCTATGATGATCAGGGAGCGGATGAGCTGACTTTCCTCGATATTA
CCGCGAGTTCCGATGACCGTGATCTGATTCTTCATATCATCGAAGAAGTGGCAGCCCAGGTATTCATCCCGTTGACGGTA
GGAGGCGGTGTTCGCAAGGTGGAAGATGTGCGCCGTCTCCTGAATGCAGGAGCTGATAAGGTAAGCATCAACACTTCGGC
CATACAAAACCCCCAACTGGTGGCTGATGCGGCGGGGCGTTATGGCTCTCAATGCATTGTCGTGGCGATAGACGCCAAGC
GTGCGGGACAAGGATGGGAAGTATTCACGCACGGGGGCCGCAAGCCTACCGGACTCGATGCAATCGAATGGGCTAAGAAA
ATGCAATCTCTCGGAGCTGGAGAAATATTGCTGACCAGTATGGACAGAGACGGTACCCGGGATGGGTTCGATCTGGCGCT
GACCCGCGCGGTGTCAGATGCGGTGGATGTACCGGTGATTGCGAGCGGCGGTGTAGGCAATCTGCAGCATCTGGTTGATG
GAGTCGTGGAAGGTCATGCCGATGCTGTGCTGGCTGCGAGCGTTTTCCACTATGGCGAATATACCGTTCGCCAGGCCAAG
GAATATATGTCACAGCATGGTATTGAAGTACGACTATAG

Upstream 100 bases:

>100_bases
CCTCCTTGCGACCCCAGAGCGCAAGGAGGGACAAAAACAGATAATCCGCATAGCGGAAACCTACAAACTTTGCGGTTTTT
AGCGGTATCCAGAGGTGTCT

Downstream 100 bases:

>100_bases
TAACAGGATCGAATCAAACTCCGGCATATTCATGTCTGATACATGGCTCAACAAAGTAAACTGGTCTGCGGACGGATTAG
TGCCGGTCGTGACACAGGAT

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MGLAKRIIPCLDVTNGRVVKGVKFVSLRDAGDPIEIARRYDDQGADELTFLDITASSDDRDLILHIIEEVAAQVFIPLTV
GGGVRKVEDVRRLLNAGADKVSINTSAIQNPQLVADAAGRYGSQCIVVAIDAKRAGQGWEVFTHGGRKPTGLDAIEWAKK
MQSLGAGEILLTSMDRDGTRDGFDLALTRAVSDAVDVPVIASGGVGNLQHLVDGVVEGHADAVLAASVFHYGEYTVRQAK
EYMSQHGIEVRL

Sequences:

>Translated_252_residues
MGLAKRIIPCLDVTNGRVVKGVKFVSLRDAGDPIEIARRYDDQGADELTFLDITASSDDRDLILHIIEEVAAQVFIPLTV
GGGVRKVEDVRRLLNAGADKVSINTSAIQNPQLVADAAGRYGSQCIVVAIDAKRAGQGWEVFTHGGRKPTGLDAIEWAKK
MQSLGAGEILLTSMDRDGTRDGFDLALTRAVSDAVDVPVIASGGVGNLQHLVDGVVEGHADAVLAASVFHYGEYTVRQAK
EYMSQHGIEVRL
>Mature_251_residues
GLAKRIIPCLDVTNGRVVKGVKFVSLRDAGDPIEIARRYDDQGADELTFLDITASSDDRDLILHIIEEVAAQVFIPLTVG
GGVRKVEDVRRLLNAGADKVSINTSAIQNPQLVADAAGRYGSQCIVVAIDAKRAGQGWEVFTHGGRKPTGLDAIEWAKKM
QSLGAGEILLTSMDRDGTRDGFDLALTRAVSDAVDVPVIASGGVGNLQHLVDGVVEGHADAVLAASVFHYGEYTVRQAKE
YMSQHGIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=42.1875, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=30.6122448979592, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.5555555555556, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_NITMU (Q2YAV0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411513.1
- ProteinModelPortal:   Q2YAV0
- SMR:   Q2YAV0
- STRING:   Q2YAV0
- GeneID:   3785858
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0814
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q2YAV0
- BioCyc:   NMUL323848:NMUL_A0814-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 2.4.2.-

Molecular weight: Translated: 26975; Mature: 26844

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAKRIIPCLDVTNGRVVKGVKFVSLRDAGDPIEIARRYDDQGADELTFLDITASSDDR
CCCHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCH
DLILHIIEEVAAQVFIPLTVGGGVRKVEDVRRLLNAGADKVSINTSAIQNPQLVADAAGR
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCCHHHHHHCC
YGSQCIVVAIDAKRAGQGWEVFTHGGRKPTGLDAIEWAKKMQSLGAGEILLTSMDRDGTR
CCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGFDLALTRAVSDAVDVPVIASGGVGNLQHLVDGVVEGHADAVLAASVFHYGEYTVRQAK
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHH
EYMSQHGIEVRL
HHHHHCCCEEEC
>Mature Secondary Structure 
GLAKRIIPCLDVTNGRVVKGVKFVSLRDAGDPIEIARRYDDQGADELTFLDITASSDDR
CCHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCH
DLILHIIEEVAAQVFIPLTVGGGVRKVEDVRRLLNAGADKVSINTSAIQNPQLVADAAGR
HHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCCHHHHHHCC
YGSQCIVVAIDAKRAGQGWEVFTHGGRKPTGLDAIEWAKKMQSLGAGEILLTSMDRDGTR
CCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCC
DGFDLALTRAVSDAVDVPVIASGGVGNLQHLVDGVVEGHADAVLAASVFHYGEYTVRQAK
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHH
EYMSQHGIEVRL
HHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA