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The map label for this gene is tuf1 [H]

Identifier: 82701885

GI number: 82701885

Start: 875181

End: 876371

Strand: Direct

Name: tuf1 [H]

Synonym: Nmul_A0752

Alternate gene names: 82701885

Gene position: 875181-876371 (Clockwise)

Preceding gene: 82701884

Following gene: 82701886

Centisome position: 27.48

GC content: 58.69

Gene sequence:

>1191_bases
ATGGCAAAGAGCAAATTTGAGCGGACGAAGCCGCACATCAACGTAGGGACGATAGGTCACGTGGACCATGGGAAGACCAC
GTTGACGGCGGCGATCACGATGGTATTGGCGAAGAAGTTTGGTGGGGAAGCGAAGAGTTACGCACAGATAGACTCGGCGC
CTGAAGAGAAGGCGCGGGGCATCACGATCAATACCTCGCACGTGGAGTACGAGACGGAGAAGCGGCATTACGCGCACGTT
GACTGTCCTGGTCACGCGGACTATGTGAAGAACATGATCACGGGTGCGGCGCAGATGGACGGTGCGATTCTGGTGGTTTC
GGCGGCGGATGGACCGATGCCGCAGACGCGGGAGCACATTCTTCTGGCGCGGCAGGTAGGGGTTCCCTACATTATTGTCT
ACATGAACAAGGCGGACATGGTGGACGATGCGGAACTTCTGGAGCTGGTGGAAATGGAAGTGCGGGAGCTGTTGTCCAAA
TACAACTTTCCGGGAGATGACACCCCGATAGTGATCGGTTCTGCACTGAAGGCGCTGGAAGGCGATCAGAGCGACATAGG
GGAGCCCTCCATCTACAAGCTTGCGGCGGCGCTGGACAGCTACATTCCGGAGCCCCAGCGGGCGGTGGACGGGGCATTTC
TGATGCCGGTCGAAGACGTTTTTTCCATATCGGGTCGTGGCACGGTGGTGACGGGTCGGGTTGAGCGTGGCGTGATCAAG
GTGGGGGAAGACATCGAGATCGTGGGATTGAAGCCCACCACCAAGACGGTGTGCACGGGTGTGGAGATGTTTCGCAAGCT
TCTGGACCAGGGGCAGGCGGGAGACAACGTGGGCGTATTGCTGCGGGGCACCAAGCGCGAGGAAGTGGAGCGTGGCCAGG
TGCTGGCCAAGCCCGGGACCATCACTCCTCATACCAAGTTCACAGCCGAGATTTACGTTCTGAGCAAGGAAGAGGGCGGG
CGTCATACTCCCTTTTTCCAGGGGTACCGGCCGCAGTTTTACTTCCGCACGACGGATGTGACGGGTGCAATCGAGTTGCC
TGCGGGCACGGAGATGGTGATGCCCGGGGACAATGTGTCGGTGACGGTAAACCTGATTGCGCCGATTGCGATGGAAGAAG
GTCTGCGTTTTGCGATTCGTGAAGGCGGCAGGACCGTGGGCGCAGGCGTCGTGGCAAAAATTATCGAATAG

Upstream 100 bases:

>100_bases
AGCACTCCCTTGGTAAGGGAGAGGTCGCCAGTTCAATCCTGGCCATGGGCTCCATAGGCGTCAATTACAGAACTCAAAAA
AATGCTTTAAAAGGGACATC

Downstream 100 bases:

>100_bases
CGTCTTGCCCGGGGTCGACTCCAGCGTTGAAGAGCCTTGCATTCGTATGTGTTTAGGTGAGTTCTTCATTTTTTACCTTT
GTTCAAAGTACAGGCCAGTA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKSKFERTKPHINVGTIGHVDHGKTTLTAAITMVLAKKFGGEAKSYAQIDSAPEEKARGITINTSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSK
YNFPGDDTPIVIGSALKALEGDQSDIGEPSIYKLAAALDSYIPEPQRAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEDIEIVGLKPTTKTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGTITPHTKFTAEIYVLSKEEGG
RHTPFFQGYRPQFYFRTTDVTGAIELPAGTEMVMPGDNVSVTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKSKFERTKPHINVGTIGHVDHGKTTLTAAITMVLAKKFGGEAKSYAQIDSAPEEKARGITINTSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSK
YNFPGDDTPIVIGSALKALEGDQSDIGEPSIYKLAAALDSYIPEPQRAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEDIEIVGLKPTTKTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGTITPHTKFTAEIYVLSKEEGG
RHTPFFQGYRPQFYFRTTDVTGAIELPAGTEMVMPGDNVSVTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKSKFERTKPHINVGTIGHVDHGKTTLTAAITMVLAKKFGGEAKSYAQIDSAPEEKARGITINTSHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKY
NFPGDDTPIVIGSALKALEGDQSDIGEPSIYKLAAALDSYIPEPQRAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEDIEIVGLKPTTKTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGTITPHTKFTAEIYVLSKEEGGR
HTPFFQGYRPQFYFRTTDVTGAIELPAGTEMVMPGDNVSVTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.3451776649746, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.4347826086957, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.4347826086957, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=29.0178571428571, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=31.1512415349887, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194097354, Length=439, Percent_Identity=29.3849658314351, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194018522, Length=439, Percent_Identity=29.3849658314351, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194018520, Length=439, Percent_Identity=29.3849658314351, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI46094014, Length=443, Percent_Identity=29.3453724604966, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=27.1676300578035, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4503507, Length=260, Percent_Identity=29.2307692307692, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=32.5925925925926, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=81.0126582278481, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=81.0126582278481, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.6296296296296, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=31.6363636363636, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789108, Length=325, Percent_Identity=27.6923076923077, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.3834586466165, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=343, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.9576837416481, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.9576837416481, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.0182232346241, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.9816513761468, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.110599078341, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=27.2727272727273, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=276, Percent_Identity=30.7971014492754, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=39.4557823129252, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=269, Percent_Identity=25.6505576208178, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.3266331658292, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=31.3769751693002, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=31.3769751693002, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=30.0595238095238, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=380, Percent_Identity=25.5263157894737, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.9245283018868, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.7430025445293, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.7430025445293, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=52.5906735751295, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.9107142857143, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.9107142857143, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=29.3986636971047, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=29.3986636971047, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=29.3986636971047, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=29.3986636971047, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=29.6551724137931, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17137380, Length=336, Percent_Identity=30.3571428571429, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=271, Percent_Identity=27.3062730627306, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647048, Length=271, Percent_Identity=27.3062730627306, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42926; Mature: 42795

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERTKPHINVGTIGHVDHGKTTLTAAITMVLAKKFGGEAKSYAQIDSAPEEKARG
CCCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHCC
ITINTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKYNFPGDDTPIVIGSALKALE
HEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHC
GDQSDIGEPSIYKLAAALDSYIPEPQRAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEDIEIVGLKPTTKTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGT
ECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECCCC
ITPHTKFTAEIYVLSKEEGGRHTPFFQGYRPQFYFRTTDVTGAIELPAGTEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCE
VTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERTKPHINVGTIGHVDHGKTTLTAAITMVLAKKFGGEAKSYAQIDSAPEEKARG
CCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHCC
ITINTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKYNFPGDDTPIVIGSALKALE
HEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHC
GDQSDIGEPSIYKLAAALDSYIPEPQRAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEDIEIVGLKPTTKTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVLAKPGT
ECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECCCC
ITPHTKFTAEIYVLSKEEGGRHTPFFQGYRPQFYFRTTDVTGAIELPAGTEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCE
VTVNLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA