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The map label for this gene is ogt [H]

Identifier: 82701880

GI number: 82701880

Start: 870855

End: 871358

Strand: Direct

Name: ogt [H]

Synonym: Nmul_A0747

Alternate gene names: 82701880

Gene position: 870855-871358 (Clockwise)

Preceding gene: 82701876

Following gene: 82701881

Centisome position: 27.35

GC content: 59.13

Gene sequence:

>504_bases
ATGATCAGACAGAAGTCGATACGGCGGGTGAAGCCGCCGGAAGATTATCAGGCCAGGCTTGCCACACCGTTTGCCGTGCT
GGGCCTTCGCACGGATGAAGACTGGCTGACCGATATAGAATACCTGCCGCCGGATACGCCCACTCTCGTTCCGCAAACTC
CTCTCGCCAGGGAAGTATGTGATCAGTTACAGGCCTACCTTGCCGATCCGGGTTTCGTGTTCGACCTGTCACTGCACATC
GGCGGGACGGTTCATCAGCGCCGTGTGTGGCAAGCCATCCAGGATATTCCGGCCGGTGCAACGCGCAGTTATGCCGCTAT
CGCGGTGGAGTTGCATTCCGCCCCGCGCGCAGTGGGGCAGGCGTGCGGAGCCAACCGCCTGCCCATCGTTATTCCCTGTC
ATCGTGTTATCGCCAAAAGCGGCGGTCTTGGAGGTTTCATGAACGCCAGCGATGGATTGCCGCTCACCATTAAGCGCTGG
CTATTGCAGCATGAGGGTATCTGA

Upstream 100 bases:

>100_bases
GATACGGGACCGTTTCCAAAAATCTCATAATGGAATTCCTGTCACTCTGTAATGCGGCTGCACCGTGAGCGGCTGTGTCG
GAGTGAGAGGGGAGGAATGG

Downstream 100 bases:

>100_bases
GCGGAACCCGGAACTGCTCGATGAATTCTGCGATGCGCTGTGGCTGGAAGATGGGCTCTCGCGCAACACAATGGAGAGCT
ATCGCAGCGATCTGGAGCAG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIRQKSIRRVKPPEDYQARLATPFAVLGLRTDEDWLTDIEYLPPDTPTLVPQTPLAREVCDQLQAYLADPGFVFDLSLHI
GGTVHQRRVWQAIQDIPAGATRSYAAIAVELHSAPRAVGQACGANRLPIVIPCHRVIAKSGGLGGFMNASDGLPLTIKRW
LLQHEGI

Sequences:

>Translated_167_residues
MIRQKSIRRVKPPEDYQARLATPFAVLGLRTDEDWLTDIEYLPPDTPTLVPQTPLAREVCDQLQAYLADPGFVFDLSLHI
GGTVHQRRVWQAIQDIPAGATRSYAAIAVELHSAPRAVGQACGANRLPIVIPCHRVIAKSGGLGGFMNASDGLPLTIKRW
LLQHEGI
>Mature_167_residues
MIRQKSIRRVKPPEDYQARLATPFAVLGLRTDEDWLTDIEYLPPDTPTLVPQTPLAREVCDQLQAYLADPGFVFDLSLHI
GGTVHQRRVWQAIQDIPAGATRSYAAIAVELHSAPRAVGQACGANRLPIVIPCHRVIAKSGGLGGFMNASDGLPLTIKRW
LLQHEGI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=169, Percent_Identity=33.1360946745562, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1788542, Length=73, Percent_Identity=49.3150684931507, Blast_Score=70, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=45.6521739130435, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=45.6521739130435, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18352; Mature: 18352

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQKSIRRVKPPEDYQARLATPFAVLGLRTDEDWLTDIEYLPPDTPTLVPQTPLAREVC
CCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHH
DQLQAYLADPGFVFDLSLHIGGTVHQRRVWQAIQDIPAGATRSYAAIAVELHSAPRAVGQ
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHH
ACGANRLPIVIPCHRVIAKSGGLGGFMNASDGLPLTIKRWLLQHEGI
HCCCCCCCEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIRQKSIRRVKPPEDYQARLATPFAVLGLRTDEDWLTDIEYLPPDTPTLVPQTPLAREVC
CCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHH
DQLQAYLADPGFVFDLSLHIGGTVHQRRVWQAIQDIPAGATRSYAAIAVELHSAPRAVGQ
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHH
ACGANRLPIVIPCHRVIAKSGGLGGFMNASDGLPLTIKRWLLQHEGI
HCCCCCCCEEEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]