Click here to switch to the map view.

The map label for this gene is iscA [H]

Identifier: 82701828

GI number: 82701828

Start: 801879

End: 802223

Strand: Direct

Name: iscA [H]

Synonym: Nmul_A0695

Alternate gene names: 82701828

Gene position: 801879-802223 (Clockwise)

Preceding gene: 82701827

Following gene: 82701829

Centisome position: 25.18

GC content: 48.99

Gene sequence:

>345_bases
ATGTCAATAACACTGACTGAAAACGCGGCAAAACAGATTCAGAAGCAGCTTGCGAAGCGGGGCAAGGGGTTGGCTCTGCG
AGTCGGGGTCAAGAAGGTGGGGTGTTCCGGATTTGCCTATACCTACGCTTATGCAGATGAGCTGCGCGAGAGTGATCGAA
TGTTCGAGTCCTGTAACGCTCATGTAGTGGTTGATGTCGACTTTCTGCCGTTTCTTGATGGTTCACGAATTGACTTTGTC
AGAGAAGGACTGAACGAATCATTCAGGTTTGAGAATCCCAATGTGGATAATACCTGCGGTTGTGGTGAAAGCTTCAGTGT
GAAAGAAGAGCCCGCCAAGGTCTAA

Upstream 100 bases:

>100_bases
CCGATCGGAGCCGCACTGCAGTTTCTGCCTGACTTCAATTCTGAATGCCGAACGCTTACTTTCGTTGTCATAATCGCATG
ATGGTAGACGGAGAATGGCG

Downstream 100 bases:

>100_bases
CCCCTGAAGTGGTGCCGGAAACCGGGATAACAGGAGCGGCAGTATTCGTAGCAAAGGAGTTGTCCTCTATTATTAATTAA
AAGAAAAGACATTCCGATAT

Product: HesB/YadR/YfhF

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MSITLTENAAKQIQKQLAKRGKGLALRVGVKKVGCSGFAYTYAYADELRESDRMFESCNAHVVVDVDFLPFLDGSRIDFV
REGLNESFRFENPNVDNTCGCGESFSVKEEPAKV

Sequences:

>Translated_114_residues
MSITLTENAAKQIQKQLAKRGKGLALRVGVKKVGCSGFAYTYAYADELRESDRMFESCNAHVVVDVDFLPFLDGSRIDFV
REGLNESFRFENPNVDNTCGCGESFSVKEEPAKV
>Mature_113_residues
SITLTENAAKQIQKQLAKRGKGLALRVGVKKVGCSGFAYTYAYADELRESDRMFESCNAHVVVDVDFLPFLDGSRIDFVR
EGLNESFRFENPNVDNTCGCGESFSVKEEPAKV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=33.9622641509434, Blast_Score=76, Evalue=6e-15,
Organism=Homo sapiens, GI160420328, Length=84, Percent_Identity=42.8571428571429, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=55.1401869158878, Blast_Score=122, Evalue=4e-30,
Organism=Escherichia coli, GI1787974, Length=106, Percent_Identity=45.2830188679245, Blast_Score=105, Evalue=7e-25,
Organism=Escherichia coli, GI1786351, Length=109, Percent_Identity=37.6146788990826, Blast_Score=72, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=38.6792452830189, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=38.6792452830189, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=38.0952380952381, Blast_Score=73, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=37.7358490566038, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24649697, Length=109, Percent_Identity=34.8623853211009, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12662; Mature: 12531

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITLTENAAKQIQKQLAKRGKGLALRVGVKKVGCSGFAYTYAYADELRESDRMFESCNA
CEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
HVVVDVDFLPFLDGSRIDFVREGLNESFRFENPNVDNTCGCGESFSVKEEPAKV
EEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SITLTENAAKQIQKQLAKRGKGLALRVGVKKVGCSGFAYTYAYADELRESDRMFESCNA
EEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
HVVVDVDFLPFLDGSRIDFVREGLNESFRFENPNVDNTCGCGESFSVKEEPAKV
EEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA