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The map label for this gene is purK [H]

Identifier: 82701744

GI number: 82701744

Start: 691745

End: 692965

Strand: Direct

Name: purK [H]

Synonym: Nmul_A0611

Alternate gene names: 82701744

Gene position: 691745-692965 (Clockwise)

Preceding gene: 82701743

Following gene: 82701745

Centisome position: 21.72

GC content: 56.92

Gene sequence:

>1221_bases
ATGAGTACGCGGCCTGGGGCGATGCTGGGGTTGCTGGGCGGCGGCCAGCTCGGCAGGATGTTCACCATGGCGGCCCAAAG
CCTGGGTTACAGCGTGACCGTTCTTGATCCCGAGGAGCGGAGCCCGGCGGGTAGCATCGCTGAGCGGCACCTGCGCGCCG
ATTACCTCGATCGCGAGGCATTGAATGAGCTCGCATCCACCTGCGCAGGCGTCACCACCGAATTCGAAAACGTACCTGCC
GAGGCACTCAGAGTACTGGCTGAACGCTGCATCGTCAGTCCCGCTGCCGAGAGTGTCGCCATCGCGCAAGATCGCATTCT
GGAGAAGAATTTTCTCGCTGCCAACGGCTTTGGGGTCGCGCCTTATACAGTGATACAGAACGCGCGCACTTCGCAGGATG
CGACCAATCCGGATGGGGCATTACCCCAGAGGCAACCGCAATTTTTTCCTGACCTCTTCCCCGGAATTCTCAAGGTAAGC
CGGTTTGGATATGACGGCAAAGGCCAGGTGCGTGTAAGTAATGCATCAGAGCTTGACAGCGCATTTGCAGGTTTGAACCG
GGAATCCTGCGTCCTGGAAAAACTGCTGCCCCTGGAGCGCGAGGTATCCGTCATCGTATCCAGGGGATTTGATGGCGAAG
TGGTTACTTTTCCCGTATCCGAGAATCAGCACTGTAACGGTATCCTCGACATCAGCATCGTTCCCGCCAGAGTATCGCCT
GAAATTGCCCGAAACGCCTGCGATATTGCGATTCGCATTGCCGAGAAGCTGGATTATCGCGGCGTATTATGCGTCGAGTT
TTTTGTGCTCGCGGAGGGACGCCTGCTGGTGAACGAGATCGCTCCTCGTCCTCATAATAGCGGGCATTACTCGATCAATG
CCTGCGTTACCTCTCAATTCGAGCAGCAGGTGCGCATACTTTGCAGAATGCCCCTCGGCAGTACTTCCATGCATGGAGCG
GCGGTAATGGTGAATCTGCTGGGCGATCTCTGGCAGAAGGGAGAGCCGGAGTGGGAACAGGTGCTGCGGCATTCCGACGT
CAAATTGCATTTATACGGCAAGCGCGATGCCAGACCCGGACGGAAAATGGGGCACTACACGGTATTGGCAGAAACGACCG
ACGCCGCGTTGCGGCTGGCGCTGGAAATCAAGCAATCGCTGCAACCCTCTCATAGTACTCTTGAAACACATGAGTCTCCC
CGGAACGAAAGTCGAAACTGA

Upstream 100 bases:

>100_bases
TTGCGCTGCTGGCGATGGAAGATGCGGGCCTGGCCAGGCAGCTTGCCGAGTTTCGGGAAACCCAGATGGCGACGGTGGCT
TCCATGACTTTACCCCTCTC

Downstream 100 bases:

>100_bases
CCAGCCCGCGCTGTTTGAAACGAGCATACAGAGTCTGCCGCTTCTGCATCGCGGAAAGGTACGGGATATCTACGGTGTGG
ATGAAGACAAGCTTTTGATC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSTRPGAMLGLLGGGQLGRMFTMAAQSLGYSVTVLDPEERSPAGSIAERHLRADYLDREALNELASTCAGVTTEFENVPA
EALRVLAERCIVSPAAESVAIAQDRILEKNFLAANGFGVAPYTVIQNARTSQDATNPDGALPQRQPQFFPDLFPGILKVS
RFGYDGKGQVRVSNASELDSAFAGLNRESCVLEKLLPLEREVSVIVSRGFDGEVVTFPVSENQHCNGILDISIVPARVSP
EIARNACDIAIRIAEKLDYRGVLCVEFFVLAEGRLLVNEIAPRPHNSGHYSINACVTSQFEQQVRILCRMPLGSTSMHGA
AVMVNLLGDLWQKGEPEWEQVLRHSDVKLHLYGKRDARPGRKMGHYTVLAETTDAALRLALEIKQSLQPSHSTLETHESP
RNESRN

Sequences:

>Translated_406_residues
MSTRPGAMLGLLGGGQLGRMFTMAAQSLGYSVTVLDPEERSPAGSIAERHLRADYLDREALNELASTCAGVTTEFENVPA
EALRVLAERCIVSPAAESVAIAQDRILEKNFLAANGFGVAPYTVIQNARTSQDATNPDGALPQRQPQFFPDLFPGILKVS
RFGYDGKGQVRVSNASELDSAFAGLNRESCVLEKLLPLEREVSVIVSRGFDGEVVTFPVSENQHCNGILDISIVPARVSP
EIARNACDIAIRIAEKLDYRGVLCVEFFVLAEGRLLVNEIAPRPHNSGHYSINACVTSQFEQQVRILCRMPLGSTSMHGA
AVMVNLLGDLWQKGEPEWEQVLRHSDVKLHLYGKRDARPGRKMGHYTVLAETTDAALRLALEIKQSLQPSHSTLETHESP
RNESRN
>Mature_405_residues
STRPGAMLGLLGGGQLGRMFTMAAQSLGYSVTVLDPEERSPAGSIAERHLRADYLDREALNELASTCAGVTTEFENVPAE
ALRVLAERCIVSPAAESVAIAQDRILEKNFLAANGFGVAPYTVIQNARTSQDATNPDGALPQRQPQFFPDLFPGILKVSR
FGYDGKGQVRVSNASELDSAFAGLNRESCVLEKLLPLEREVSVIVSRGFDGEVVTFPVSENQHCNGILDISIVPARVSPE
IARNACDIAIRIAEKLDYRGVLCVEFFVLAEGRLLVNEIAPRPHNSGHYSINACVTSQFEQQVRILCRMPLGSTSMHGAA
VMVNLLGDLWQKGEPEWEQVLRHSDVKLHLYGKRDARPGRKMGHYTVLAETTDAALRLALEIKQSLQPSHSTLETHESPR
NESRN

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=379, Percent_Identity=31.1345646437995, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788155, Length=393, Percent_Identity=24.6819338422392, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=377, Percent_Identity=33.42175066313, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 44430; Mature: 44299

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRPGAMLGLLGGGQLGRMFTMAAQSLGYSVTVLDPEERSPAGSIAERHLRADYLDREA
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LNELASTCAGVTTEFENVPAEALRVLAERCIVSPAAESVAIAQDRILEKNFLAANGFGVA
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEECCCCCC
PYTVIQNARTSQDATNPDGALPQRQPQFFPDLFPGILKVSRFGYDGKGQVRVSNASELDS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHH
AFAGLNRESCVLEKLLPLEREVSVIVSRGFDGEVVTFPVSENQHCNGILDISIVPARVSP
HHHCCCHHHHHHHHHCCCHHHHHHHEECCCCCCEEEEECCCCCCCCEEEEEEEECCCCCH
EIARNACDIAIRIAEKLDYRGVLCVEFFVLAEGRLLVNEIAPRPHNSGHYSINACVTSQF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEHHCCCCCCCCCCEEEEHHHHHHH
EQQVRILCRMPLGSTSMHGAAVMVNLLGDLWQKGEPEWEQVLRHSDVKLHLYGKRDARPG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCC
RKMGHYTVLAETTDAALRLALEIKQSLQPSHSTLETHESPRNESRN
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STRPGAMLGLLGGGQLGRMFTMAAQSLGYSVTVLDPEERSPAGSIAERHLRADYLDREA
CCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LNELASTCAGVTTEFENVPAEALRVLAERCIVSPAAESVAIAQDRILEKNFLAANGFGVA
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEECCCCCC
PYTVIQNARTSQDATNPDGALPQRQPQFFPDLFPGILKVSRFGYDGKGQVRVSNASELDS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHH
AFAGLNRESCVLEKLLPLEREVSVIVSRGFDGEVVTFPVSENQHCNGILDISIVPARVSP
HHHCCCHHHHHHHHHCCCHHHHHHHEECCCCCCEEEEECCCCCCCCEEEEEEEECCCCCH
EIARNACDIAIRIAEKLDYRGVLCVEFFVLAEGRLLVNEIAPRPHNSGHYSINACVTSQF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEHHCCCCCCCCCCEEEEHHHHHHH
EQQVRILCRMPLGSTSMHGAAVMVNLLGDLWQKGEPEWEQVLRHSDVKLHLYGKRDARPG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCC
RKMGHYTVLAETTDAALRLALEIKQSLQPSHSTLETHESPRNESRN
CCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]