Click here to switch to the map view.

The map label for this gene is aspS

Identifier: 82701736

GI number: 82701736

Start: 684314

End: 686080

Strand: Direct

Name: aspS

Synonym: Nmul_A0603

Alternate gene names: 82701736

Gene position: 684314-686080 (Clockwise)

Preceding gene: 82701735

Following gene: 82701737

Centisome position: 21.49

GC content: 56.54

Gene sequence:

>1767_bases
ATGCGCACCAATTACTGCGGCCTTATCGACGTCCAATATCTGGATCAGACTGTGATTCTGTACGGCTGGGTGCACCGGCG
CCGTGACCATGGGGGAGTTATTTTCGTGGACCTGCGGGATCGTGAGGGCTTGGTGCAGGTGGTGTGCGACCCCGATAACC
CCACTGCTTTCCAGGTCGCCGAAAAAATCCGGAACGAGTTTGTGGTCCGGATTACCGGACGTGTGCGGCATCGCCCGCCC
GGAACCGTCAACCCCAATCTCATCAGCGGGGAAATAGAGGTGTTGGTGAGCTCGATCGATATCCTTAATGCTTCCCTGAC
GCCTCCGTTCCTGATGGACGACGAAAGCCTCAGCGAAGCCACACGGCTGGAACACCGCTATCTGGATCTGCGGCGTCCGC
AGATGCAGGAAAACCTGCGGTTGCGCTACAAGGTAGCGATGGCTGTCCGGCTGTTTCTCGACCAGCATGGATTCATCGAC
GTGGAAACGCCGATGCTCACCAAATCGACTCCCGAAGGCGCGCGCGATTACCTAGTGCCGTCACGCGTCAACACCGGGCA
TTTTTTTGCGCTGCCGCAGTCGCCGCAACTGTTCAAGCAGCTGCTGATGGTTTCCGGATTCGACCGCTATTATCAGATTA
CCAAATGCTTTCGCGATGAGGATCTGCGGGCTGACCGCCAGCCCGAATTCACCCAGATCGATATCGAGACATCCTTTCTG
AATGAATCACAAATCATGCAGATGATGGAAAGGATGATCTGCACGGTGTTCAAAAGCGTGAGGAATATCGATCTCCCCAC
TCCCTTTCCACGCCTGACGCATGGCGAAGCGATGTCGAAATATGGCTCGGACAAGCCTGACATGCGTGTGTCGCTGGTTC
TGACGGAACTCACCGATGTGATGCAGGAGGTGGAGTTCAAGGTGTTCCGGGAAGCCGCGCTCAGAACGGGGGGACGTGTA
GCGGCATTGCGCGTGCCTGGAGGTGGGGCATTGTCGCGCAAAGAGATCGATGATTACACCGGTTTTGTCGCGATATACGG
GGCGAAGGGGCTCGCATACATCAAGGTCAATGCGCTGGAAAAAGGCATGGACGGCCTCCAGTCGCCCATACTGAAATTCC
TCCCTGAGAATGTGATTCAGATCGTTCTCGAGCGCACGGGGGCGAAGGATGGGGATCTCATCTTCTTCGGCGCGGACAAG
GCACAGGTTGTGAATGAGGCGCTCGGTGCATTGCGCGCGAAGGTGGGGCATGATCGCGGCCTGGCGGAATCGGGATGGAA
GCCGTTATGGGTGGTGGATTTCCCCATGTTCGAGCGCGACGAAGAGGAAAACCGCTGGAAGGCCTTGCACCATCCCTTTA
CTTCGCCTGCCGAGGGTCACGAGGACCTGCTTGAAACCGATCCCGAAAAGGCCTTATCCAAGGCTTACGATATGGTGCTG
AATGGCTCGGAGATTGGCGGCGGTTCCGTACGTATTCATCGCCAGGAAGTACAGTCGAAGGTATTTCGCGCCTTGAATAT
TGGAGCGGACGAGGCAAACGAAAAATTCGGCTTTTTGCTCGAAGCATTGCAATATGGGGCCCCTCCGCATGGCGGGATTG
CGTTCGGCCTCGACCGGATCGTTGCGATGATGGCAGGCGCCGAATCCATCCGTGAGGTCATTGCTTTTCCGAAAACACAG
CGTGCGCAATGTCTGCTGACGCATGCGCCGAGTACTGTCGGTGAGAAACAGTTGCGGGAACTTCACATCAAGCTTCGCCA
CGCGTAA

Upstream 100 bases:

>100_bases
AACCGGTGGAGCCGCAGGCAGCGCTGCTGCATCCGCAGGACTCCGTCAGTTCTGCATCGAGCGACAAGTCCTGATTCCTT
TTTTCTGATTACCGATCTCC

Downstream 100 bases:

>100_bases
TCCTGGAGGATATGAAGCAAGGGCGCGGGGTGGAAGCGCAGAAGCGTGGAGATTGGGAAAATCGTTTTTCCGAAAGCGAA
GATTTTCTGATTCCGTTCCT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRTNYCGLIDVQYLDQTVILYGWVHRRRDHGGVIFVDLRDREGLVQVVCDPDNPTAFQVAEKIRNEFVVRITGRVRHRPP
GTVNPNLISGEIEVLVSSIDILNASLTPPFLMDDESLSEATRLEHRYLDLRRPQMQENLRLRYKVAMAVRLFLDQHGFID
VETPMLTKSTPEGARDYLVPSRVNTGHFFALPQSPQLFKQLLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NESQIMQMMERMICTVFKSVRNIDLPTPFPRLTHGEAMSKYGSDKPDMRVSLVLTELTDVMQEVEFKVFREAALRTGGRV
AALRVPGGGALSRKEIDDYTGFVAIYGAKGLAYIKVNALEKGMDGLQSPILKFLPENVIQIVLERTGAKDGDLIFFGADK
AQVVNEALGALRAKVGHDRGLAESGWKPLWVVDFPMFERDEEENRWKALHHPFTSPAEGHEDLLETDPEKALSKAYDMVL
NGSEIGGGSVRIHRQEVQSKVFRALNIGADEANEKFGFLLEALQYGAPPHGGIAFGLDRIVAMMAGAESIREVIAFPKTQ
RAQCLLTHAPSTVGEKQLRELHIKLRHA

Sequences:

>Translated_588_residues
MRTNYCGLIDVQYLDQTVILYGWVHRRRDHGGVIFVDLRDREGLVQVVCDPDNPTAFQVAEKIRNEFVVRITGRVRHRPP
GTVNPNLISGEIEVLVSSIDILNASLTPPFLMDDESLSEATRLEHRYLDLRRPQMQENLRLRYKVAMAVRLFLDQHGFID
VETPMLTKSTPEGARDYLVPSRVNTGHFFALPQSPQLFKQLLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NESQIMQMMERMICTVFKSVRNIDLPTPFPRLTHGEAMSKYGSDKPDMRVSLVLTELTDVMQEVEFKVFREAALRTGGRV
AALRVPGGGALSRKEIDDYTGFVAIYGAKGLAYIKVNALEKGMDGLQSPILKFLPENVIQIVLERTGAKDGDLIFFGADK
AQVVNEALGALRAKVGHDRGLAESGWKPLWVVDFPMFERDEEENRWKALHHPFTSPAEGHEDLLETDPEKALSKAYDMVL
NGSEIGGGSVRIHRQEVQSKVFRALNIGADEANEKFGFLLEALQYGAPPHGGIAFGLDRIVAMMAGAESIREVIAFPKTQ
RAQCLLTHAPSTVGEKQLRELHIKLRHA
>Mature_588_residues
MRTNYCGLIDVQYLDQTVILYGWVHRRRDHGGVIFVDLRDREGLVQVVCDPDNPTAFQVAEKIRNEFVVRITGRVRHRPP
GTVNPNLISGEIEVLVSSIDILNASLTPPFLMDDESLSEATRLEHRYLDLRRPQMQENLRLRYKVAMAVRLFLDQHGFID
VETPMLTKSTPEGARDYLVPSRVNTGHFFALPQSPQLFKQLLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
NESQIMQMMERMICTVFKSVRNIDLPTPFPRLTHGEAMSKYGSDKPDMRVSLVLTELTDVMQEVEFKVFREAALRTGGRV
AALRVPGGGALSRKEIDDYTGFVAIYGAKGLAYIKVNALEKGMDGLQSPILKFLPENVIQIVLERTGAKDGDLIFFGADK
AQVVNEALGALRAKVGHDRGLAESGWKPLWVVDFPMFERDEEENRWKALHHPFTSPAEGHEDLLETDPEKALSKAYDMVL
NGSEIGGGSVRIHRQEVQSKVFRALNIGADEANEKFGFLLEALQYGAPPHGGIAFGLDRIVAMMAGAESIREVIAFPKTQ
RAQCLLTHAPSTVGEKQLRELHIKLRHA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=40.9698996655518, Blast_Score=431, Evalue=1e-120,
Organism=Homo sapiens, GI45439306, Length=275, Percent_Identity=26.9090909090909, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI5031815, Length=289, Percent_Identity=24.2214532871972, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI194272210, Length=289, Percent_Identity=24.2214532871972, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=56.8259385665529, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=294, Percent_Identity=25.5102040816327, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=26.530612244898, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=35.8931552587646, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=310, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535927, Length=310, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=310, Percent_Identity=24.1935483870968, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=25.5395683453237, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=293, Percent_Identity=26.6211604095563, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=299, Percent_Identity=37.4581939799331, Blast_Score=189, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=284, Percent_Identity=26.4084507042254, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=261, Percent_Identity=26.0536398467433, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=287, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=37.8512396694215, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640849, Length=251, Percent_Identity=29.0836653386454, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640851, Length=251, Percent_Identity=29.0836653386454, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17136276, Length=268, Percent_Identity=27.2388059701493, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921528, Length=251, Percent_Identity=27.4900398406374, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_NITMU (Q2YBG1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411302.1
- ProteinModelPortal:   Q2YBG1
- SMR:   Q2YBG1
- STRING:   Q2YBG1
- GeneID:   3786678
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0603
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q2YBG1
- ProtClustDB:   PRK00476
- BioCyc:   NMUL323848:NMUL_A0603-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66319; Mature: 66319

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNYCGLIDVQYLDQTVILYGWVHRRRDHGGVIFVDLRDREGLVQVVCDPDNPTAFQVA
CCCCCCCEEEHHHHCCCCEEEHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHH
EKIRNEFVVRITGRVRHRPPGTVNPNLISGEIEVLVSSIDILNASLTPPFLMDDESLSEA
HHHHHCEEEEEECCEECCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCEEECCHHHHHH
TRLEHRYLDLRRPQMQENLRLRYKVAMAVRLFLDQHGFIDVETPMLTKSTPEGARDYLVP
HHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHCCCC
SRVNTGHFFALPQSPQLFKQLLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
CCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHC
NESQIMQMMERMICTVFKSVRNIDLPTPFPRLTHGEAMSKYGSDKPDMRVSLVLTELTDV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHHH
MQEVEFKVFREAALRTGGRVAALRVPGGGALSRKEIDDYTGFVAIYGAKGLAYIKVNALE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCEEEEECCCCEEEEEEEHHH
KGMDGLQSPILKFLPENVIQIVLERTGAKDGDLIFFGADKAQVVNEALGALRAKVGHDRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LAESGWKPLWVVDFPMFERDEEENRWKALHHPFTSPAEGHEDLLETDPEKALSKAYDMVL
CHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHC
NGSEIGGGSVRIHRQEVQSKVFRALNIGADEANEKFGFLLEALQYGAPPHGGIAFGLDRI
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
VAMMAGAESIREVIAFPKTQRAQCLLTHAPSTVGEKQLRELHIKLRHA
HHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTNYCGLIDVQYLDQTVILYGWVHRRRDHGGVIFVDLRDREGLVQVVCDPDNPTAFQVA
CCCCCCCEEEHHHHCCCCEEEHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHH
EKIRNEFVVRITGRVRHRPPGTVNPNLISGEIEVLVSSIDILNASLTPPFLMDDESLSEA
HHHHHCEEEEEECCEECCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCEEECCHHHHHH
TRLEHRYLDLRRPQMQENLRLRYKVAMAVRLFLDQHGFIDVETPMLTKSTPEGARDYLVP
HHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHCCCC
SRVNTGHFFALPQSPQLFKQLLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDIETSFL
CCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHC
NESQIMQMMERMICTVFKSVRNIDLPTPFPRLTHGEAMSKYGSDKPDMRVSLVLTELTDV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEHHHHHHHHH
MQEVEFKVFREAALRTGGRVAALRVPGGGALSRKEIDDYTGFVAIYGAKGLAYIKVNALE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCEEEEECCCCEEEEEEEHHH
KGMDGLQSPILKFLPENVIQIVLERTGAKDGDLIFFGADKAQVVNEALGALRAKVGHDRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LAESGWKPLWVVDFPMFERDEEENRWKALHHPFTSPAEGHEDLLETDPEKALSKAYDMVL
CHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHC
NGSEIGGGSVRIHRQEVQSKVFRALNIGADEANEKFGFLLEALQYGAPPHGGIAFGLDRI
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
VAMMAGAESIREVIAFPKTQRAQCLLTHAPSTVGEKQLRELHIKLRHA
HHHHHCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA