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The map label for this gene is mutM

Identifier: 82701716

GI number: 82701716

Start: 666278

End: 667096

Strand: Reverse

Name: mutM

Synonym: Nmul_A0582

Alternate gene names: 82701716

Gene position: 667096-666278 (Counterclockwise)

Preceding gene: 82701717

Following gene: 82701715

Centisome position: 20.95

GC content: 57.02

Gene sequence:

>819_bases
TTGCCTGAACTGCCTGAAGTCGAAGTTGTCCGTCGCGGCATCGCCTCTGGACTCGAGGGACGCAAGATCGCCGGCTTGAC
GGTACGAAACCCGAATCTACGCTGGCCGGTGCCGGAACTTGAGCGAACGCTTTGCGGCCTGGAAATCAGAACGGTAACGC
GTCGCGGAAAATACCTCTTGCTCGACTGCGGTGCTGGCACGCTGATCCTGCATCTCGGCATGTCCGGCAGCTTGAGATTG
CTGGCCCTTGCAGCTAATATCGCTCCACAGAAGCACGATCATATCGATCTGCTCCTGGATAAAGGCATGGTTCTCCGTTT
TCGGGATCCCCGTCGTTTTGGGGCTGTGCTGTGGACTACGGGAAATGCCACCGACCACCCCCTGCTGTCTCACTTGGGAC
CCGAACCCCTTACCGAAGCGTTCGACGGCAAGCTGCTTTATAGCAAAACCCGAAACCGTAGCGCCAGCATTAAAGAGGTA
TTGATGAACAGCCGCATTGTCGTCGGCGTCGGAAACATTTACGCAAACGAAGCGCTCTTTCTTGCCGGTATTGATCCCGC
CAACGCTGCCGGCCGGATTGGAGCGAGAAGATGCGCGGGATTGGCGCAGGCAATCAAGGAAACCCTCGGGCGCGCGATAG
AAGCAGGCGGCAGCAGCTTGCGCGATTTCGTTGGCAGCGACGGCAATCCCGGATATTTCCAGCAGCAGTATTGGGTTTAT
AGCCGGACCGGGCAGCCTTGCCGGAAATGTGGTACAAATATCGAACAGATCAGGCAGGGGCAGCGTTCGAGCTTTTATTG
CCCGCGTTGCCAGAAATAA

Upstream 100 bases:

>100_bases
ACCGATCAGTTCGGTGAATCCGGAATCCTGGACAGTGTTCCCCCTCCCTTGACCATACCCGGCGACCTCTTTATCCCAAC
ATTCCATCCCGACATTCGAA

Downstream 100 bases:

>100_bases
ATTCACTGGATACATGAATATTACATTCATTGGTGGCGGCAACATGGCTTGCGCTTTGATCGGCGGGCTGCTGCAACAGG
ATTATTCACCGGCGCAGATA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPELPEVEVVRRGIASGLEGRKIAGLTVRNPNLRWPVPELERTLCGLEIRTVTRRGKYLLLDCGAGTLILHLGMSGSLRL
LALAANIAPQKHDHIDLLLDKGMVLRFRDPRRFGAVLWTTGNATDHPLLSHLGPEPLTEAFDGKLLYSKTRNRSASIKEV
LMNSRIVVGVGNIYANEALFLAGIDPANAAGRIGARRCAGLAQAIKETLGRAIEAGGSSLRDFVGSDGNPGYFQQQYWVY
SRTGQPCRKCGTNIEQIRQGQRSSFYCPRCQK

Sequences:

>Translated_272_residues
MPELPEVEVVRRGIASGLEGRKIAGLTVRNPNLRWPVPELERTLCGLEIRTVTRRGKYLLLDCGAGTLILHLGMSGSLRL
LALAANIAPQKHDHIDLLLDKGMVLRFRDPRRFGAVLWTTGNATDHPLLSHLGPEPLTEAFDGKLLYSKTRNRSASIKEV
LMNSRIVVGVGNIYANEALFLAGIDPANAAGRIGARRCAGLAQAIKETLGRAIEAGGSSLRDFVGSDGNPGYFQQQYWVY
SRTGQPCRKCGTNIEQIRQGQRSSFYCPRCQK
>Mature_271_residues
PELPEVEVVRRGIASGLEGRKIAGLTVRNPNLRWPVPELERTLCGLEIRTVTRRGKYLLLDCGAGTLILHLGMSGSLRLL
ALAANIAPQKHDHIDLLLDKGMVLRFRDPRRFGAVLWTTGNATDHPLLSHLGPEPLTEAFDGKLLYSKTRNRSASIKEVL
MNSRIVVGVGNIYANEALFLAGIDPANAAGRIGARRCAGLAQAIKETLGRAIEAGGSSLRDFVGSDGNPGYFQQQYWVYS
RTGQPCRKCGTNIEQIRQGQRSSFYCPRCQK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=272, Percent_Identity=51.8382352941177, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1786932, Length=277, Percent_Identity=24.5487364620939, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_NITMU (Q2YBI1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411282.1
- HSSP:   P42371
- ProteinModelPortal:   Q2YBI1
- SMR:   Q2YBI1
- STRING:   Q2YBI1
- GeneID:   3783980
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0582
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- PhylomeDB:   Q2YBI1
- ProtClustDB:   PRK01103
- BioCyc:   NMUL323848:NMUL_A0582-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29848; Mature: 29717

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 57-57 ACT_SITE 262-262 BINDING 92-92 BINDING 111-111 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVVRRGIASGLEGRKIAGLTVRNPNLRWPVPELERTLCGLEIRTVTRRGKYLL
CCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCEEE
LDCGAGTLILHLGMSGSLRLLALAANIAPQKHDHIDLLLDKGMVLRFRDPRRFGAVLWTT
EECCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCEEEEECCCEEEEEECCCHHCEEEEEC
GNATDHPLLSHLGPEPLTEAFDGKLLYSKTRNRSASIKEVLMNSRIVVGVGNIYANEALF
CCCCCCCHHHHCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHCCEEEEEECCEECCCEEE
LAGIDPANAAGRIGARRCAGLAQAIKETLGRAIEAGGSSLRDFVGSDGNPGYFQQQYWVY
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEE
SRTGQPCRKCGTNIEQIRQGQRSSFYCPRCQK
ECCCCHHHHHCCCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVEVVRRGIASGLEGRKIAGLTVRNPNLRWPVPELERTLCGLEIRTVTRRGKYLL
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCEEE
LDCGAGTLILHLGMSGSLRLLALAANIAPQKHDHIDLLLDKGMVLRFRDPRRFGAVLWTT
EECCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCEEEEECCCEEEEEECCCHHCEEEEEC
GNATDHPLLSHLGPEPLTEAFDGKLLYSKTRNRSASIKEVLMNSRIVVGVGNIYANEALF
CCCCCCCHHHHCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHCCEEEEEECCEECCCEEE
LAGIDPANAAGRIGARRCAGLAQAIKETLGRAIEAGGSSLRDFVGSDGNPGYFQQQYWVY
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEE
SRTGQPCRKCGTNIEQIRQGQRSSFYCPRCQK
ECCCCHHHHHCCCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA