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The map label for this gene is lpdA [H]

Identifier: 82701649

GI number: 82701649

Start: 583866

End: 585746

Strand: Direct

Name: lpdA [H]

Synonym: Nmul_A0515

Alternate gene names: 82701649

Gene position: 583866-585746 (Clockwise)

Preceding gene: 82701648

Following gene: 82701656

Centisome position: 18.34

GC content: 55.87

Gene sequence:

>1881_bases
ATGGCTCAGCTTACCGAAATCAAAGTACCGGATATTGGCGACTTCAAGGATGTCGCAGTGATTGAAGTGCTGGTGAAACC
CGGCGATACAGTGGAAAAGGAAACATCGCTTATCACCCTGGAAACCGATAAGGCATCCATTGAAGTTCCTTCACCCCAAA
GTGGCATTGTGAAGGAACTTAAAGTCAAGGTTGGCGACAAGGTTTCGGAAGGCTCGATCATACTCATGCTGGAGGCCACA
ACAGCAGAAGCGGCTGCTCCTGCTAAAGCCTCTGAACCTGGTGGTGCGAGCAAGCAGGAAGAGCCGGCTGAGAAGCCCGC
TGCCAACGTTGCTCCTCCATCGGAAAAAGAGGAAGAAGAGAAAACGCCGGCCTCAGTCGCTGCTCCTGCTCCTGAATCCG
TAGCACCACCCGCTGCACCTTCGATTCCACAAGGTGATATTCATGCCGATATAGTGGTATTGGGTGCAGGGCCGGGTGGC
TATACCGCCGCCTTTCGTGCTGCCGATCTCGGCAAGAATGTGGTCTTGATCGAGCGCTACTCCACGCTTGGCGGCGTCTG
TCTCAATGTGGGCTGCATTCCCTCCAAGGCGCTGCTGCATGTGGCGAAAGTCATCACCGACGCCGAGGAAACAGCGCAAC
AGGGTATCGCTTTCGCTAAGCCCGGCATAGAAATAGACAAACTGCGAGGATGGAAGGAATCCATCATCGGCAAACTCACC
AAGGGATTGACGGGGCTGGCGAAGCAACGCAAGGTGAAGGTAGTGCGGGGAACGGGCAGGTTCACTTCGCCGAATATGAT
CGAAGTGGAAACCTCCGACGGAAAAAAAACAGTGTCGTTCGAGCACTGCATCATTGCCGCGGGTTCTGCTGCCGCTCGCA
TTCCCGGTTTCCCCTACGATGACCCGCGGATCATCGATTCCACTGGCGCGCTCAAGCTGGAGAGCATCCCCAAGCGCATG
CTGATCATCGGGGGCGGCATTATCGGTCTCGAAATGGCGACGGTCTACGATGCGCTCGGCAGCAGGATCAGTGTCGTCGA
ATTGATGGATCAATTGATTCCCGGCGCGGATGCCGATCTTATCCGGCCGCTGCACAAACGCATACAGAAACGCTACGAGG
CCATCTACCTCAAAACCAAAGTCACCAGAATTGAAGCGCTGCAAGAAGGATTGAGAGTCACGTTCGAAGGATCCTCGGAA
GGTGGCGGCCCAGAGGGCACTGGAGCACCCGAACCGCAAGTATATGACCGGATCCTGATGGCGGTAGGACGCCGGCCAAA
TGGCCGCGAAATTGGGGCAGAAAAGGCAGGTATAGCTGTGAACGAGCGTGGCTTCATCCCGGTGGATAAGCAGTTGCGGA
CCAACGTCTCTCACATTTTCGCCATAGGCGACATCGCGGGCGAGCCCATGCTGGCGCACAAGGCATCGCATGAAGGCAAA
CTGGCGGCGGAAATCATCGCCGGCGGGGAGAAGATGAAGTCAGCAGCGTTCGACGCTCGCGCCATTCCCTCGGTAGCTTA
CACCGATCCTGAGATCGCCTGGATGGGCCTCACCGAAACCGAAGCCAAAAAGCAAGGTATCGAAATCGAAAAAGCGGTAT
TTCCGTGGGCCGTCAGCGGTCGTGCTTTGGCAATGGCGCGCGACGAGGGCATGACAAAGTTAATCCTGGACAAGAAGACG
CGGCGCATTCTCGGCGCGGGCATCGTAGGCATCAATGCCGGCGAACTGATATCCGAAACCGTACTGGGATTGGAAATGGG
CGCGGATATGGAAGATATCGGCCTTACCATCCATCCGCATCCGACTCTATCCGAAACAGTGTTCTTTGCCGCTGAGATCG
CGGAGGGGACGATTACCGATCTTTATATGCCGAAGAAGTAG

Upstream 100 bases:

>100_bases
TGACCAGCCAGAAATTCATCGTCCTGGGTGACGGGCATATCCGGCAGTAGGTTTCCAGGAATAAGGTTCAATTTTATTTC
CTTAAACTAATAAGGTTTTC

Downstream 100 bases:

>100_bases
AGGATCAATGGGGTCGGAGCCGATTGACTTTCAGAAATCAGTCGACTCCGACTCCTTGATTTTGGCTCAGGCCACCGCCC
TTTTAAGTTGAAGTCTCGCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MAQLTEIKVPDIGDFKDVAVIEVLVKPGDTVEKETSLITLETDKASIEVPSPQSGIVKELKVKVGDKVSEGSIILMLEAT
TAEAAAPAKASEPGGASKQEEPAEKPAANVAPPSEKEEEEKTPASVAAPAPESVAPPAAPSIPQGDIHADIVVLGAGPGG
YTAAFRAADLGKNVVLIERYSTLGGVCLNVGCIPSKALLHVAKVITDAEETAQQGIAFAKPGIEIDKLRGWKESIIGKLT
KGLTGLAKQRKVKVVRGTGRFTSPNMIEVETSDGKKTVSFEHCIIAAGSAAARIPGFPYDDPRIIDSTGALKLESIPKRM
LIIGGGIIGLEMATVYDALGSRISVVELMDQLIPGADADLIRPLHKRIQKRYEAIYLKTKVTRIEALQEGLRVTFEGSSE
GGGPEGTGAPEPQVYDRILMAVGRRPNGREIGAEKAGIAVNERGFIPVDKQLRTNVSHIFAIGDIAGEPMLAHKASHEGK
LAAEIIAGGEKMKSAAFDARAIPSVAYTDPEIAWMGLTETEAKKQGIEIEKAVFPWAVSGRALAMARDEGMTKLILDKKT
RRILGAGIVGINAGELISETVLGLEMGADMEDIGLTIHPHPTLSETVFFAAEIAEGTITDLYMPKK

Sequences:

>Translated_626_residues
MAQLTEIKVPDIGDFKDVAVIEVLVKPGDTVEKETSLITLETDKASIEVPSPQSGIVKELKVKVGDKVSEGSIILMLEAT
TAEAAAPAKASEPGGASKQEEPAEKPAANVAPPSEKEEEEKTPASVAAPAPESVAPPAAPSIPQGDIHADIVVLGAGPGG
YTAAFRAADLGKNVVLIERYSTLGGVCLNVGCIPSKALLHVAKVITDAEETAQQGIAFAKPGIEIDKLRGWKESIIGKLT
KGLTGLAKQRKVKVVRGTGRFTSPNMIEVETSDGKKTVSFEHCIIAAGSAAARIPGFPYDDPRIIDSTGALKLESIPKRM
LIIGGGIIGLEMATVYDALGSRISVVELMDQLIPGADADLIRPLHKRIQKRYEAIYLKTKVTRIEALQEGLRVTFEGSSE
GGGPEGTGAPEPQVYDRILMAVGRRPNGREIGAEKAGIAVNERGFIPVDKQLRTNVSHIFAIGDIAGEPMLAHKASHEGK
LAAEIIAGGEKMKSAAFDARAIPSVAYTDPEIAWMGLTETEAKKQGIEIEKAVFPWAVSGRALAMARDEGMTKLILDKKT
RRILGAGIVGINAGELISETVLGLEMGADMEDIGLTIHPHPTLSETVFFAAEIAEGTITDLYMPKK
>Mature_625_residues
AQLTEIKVPDIGDFKDVAVIEVLVKPGDTVEKETSLITLETDKASIEVPSPQSGIVKELKVKVGDKVSEGSIILMLEATT
AEAAAPAKASEPGGASKQEEPAEKPAANVAPPSEKEEEEKTPASVAAPAPESVAPPAAPSIPQGDIHADIVVLGAGPGGY
TAAFRAADLGKNVVLIERYSTLGGVCLNVGCIPSKALLHVAKVITDAEETAQQGIAFAKPGIEIDKLRGWKESIIGKLTK
GLTGLAKQRKVKVVRGTGRFTSPNMIEVETSDGKKTVSFEHCIIAAGSAAARIPGFPYDDPRIIDSTGALKLESIPKRML
IIGGGIIGLEMATVYDALGSRISVVELMDQLIPGADADLIRPLHKRIQKRYEAIYLKTKVTRIEALQEGLRVTFEGSSEG
GGPEGTGAPEPQVYDRILMAVGRRPNGREIGAEKAGIAVNERGFIPVDKQLRTNVSHIFAIGDIAGEPMLAHKASHEGKL
AAEIIAGGEKMKSAAFDARAIPSVAYTDPEIAWMGLTETEAKKQGIEIEKAVFPWAVSGRALAMARDEGMTKLILDKKTR
RILGAGIVGINAGELISETVLGLEMGADMEDIGLTIHPHPTLSETVFFAAEIAEGTITDLYMPKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=39.7463002114165, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=28.6637931034483, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519428, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519426, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI291045266, Length=489, Percent_Identity=28.0163599182004, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=27.6955602536998, Blast_Score=124, Evalue=4e-28,
Organism=Homo sapiens, GI291045268, Length=484, Percent_Identity=26.2396694214876, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI1786307, Length=481, Percent_Identity=59.043659043659, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=28.9130434782609, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=26.9230769230769, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789915, Length=456, Percent_Identity=26.7543859649123, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI1786305, Length=72, Percent_Identity=63.8888888888889, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=39.8720682302772, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=491, Percent_Identity=29.1242362525458, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=448, Percent_Identity=25.4464285714286, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=496, Percent_Identity=27.4193548387097, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=476, Percent_Identity=26.0504201680672, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=40.552016985138, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=29.8755186721992, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.8131634819533, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=39.9568034557235, Blast_Score=328, Evalue=8e-90,
Organism=Drosophila melanogaster, GI24640551, Length=589, Percent_Identity=25.9762308998302, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=27.0103092783505, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=26.7206477732794, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=25.5060728744939, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 66160; Mature: 66029

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLTEIKVPDIGDFKDVAVIEVLVKPGDTVEKETSLITLETDKASIEVPSPQSGIVKEL
CCCCCEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
KVKVGDKVSEGSIILMLEATTAEAAAPAKASEPGGASKQEEPAEKPAANVAPPSEKEEEE
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KTPASVAAPAPESVAPPAAPSIPQGDIHADIVVLGAGPGGYTAAFRAADLGKNVVLIERY
CCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHEEHHHCCCCEEEEEEC
STLGGVCLNVGCIPSKALLHVAKVITDAEETAQQGIAFAKPGIEIDKLRGWKESIIGKLT
CCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHCCCEEECCCCCHHHHCCHHHHHHHHHH
KGLTGLAKQRKVKVVRGTGRFTSPNMIEVETSDGKKTVSFEHCIIAAGSAAARIPGFPYD
HHHHHHHHHCEEEEEECCCCCCCCCEEEEECCCCCEEEEECEEEEECCCCHHCCCCCCCC
DPRIIDSTGALKLESIPKRMLIIGGGIIGLEMATVYDALGSRISVVELMDQLIPGADADL
CCEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
IRPLHKRIQKRYEAIYLKTKVTRIEALQEGLRVTFEGSSEGGGPEGTGAPEPQVYDRILM
HHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
AVGRRPNGREIGAEKAGIAVNERGFIPVDKQLRTNVSHIFAIGDIAGEPMLAHKASHEGK
HHCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHCCHHHEEEEECCCCCCHHHHCCCCCCC
LAAEIIAGGEKMKSAAFDARAIPSVAYTDPEIAWMGLTETEAKKQGIEIEKAVFPWAVSG
CHHHHHHCCHHHHHHHCCHHCCCCEEECCCCEEEECCCHHHHHHCCCEEEHEECCEEECC
RALAMARDEGMTKLILDKKTRRILGAGIVGINAGELISETVLGLEMGADMEDIGLTIHPH
CEEEEECCCCCEEEEHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCHHHCCEEECCC
PTLSETVFFAAEIAEGTITDLYMPKK
CCHHHHHEEEEHHCCCCEEEEECCCC
>Mature Secondary Structure 
AQLTEIKVPDIGDFKDVAVIEVLVKPGDTVEKETSLITLETDKASIEVPSPQSGIVKEL
CCCCEECCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHH
KVKVGDKVSEGSIILMLEATTAEAAAPAKASEPGGASKQEEPAEKPAANVAPPSEKEEEE
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KTPASVAAPAPESVAPPAAPSIPQGDIHADIVVLGAGPGGYTAAFRAADLGKNVVLIERY
CCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHEEHHHCCCCEEEEEEC
STLGGVCLNVGCIPSKALLHVAKVITDAEETAQQGIAFAKPGIEIDKLRGWKESIIGKLT
CCCCCEEEEECCCCHHHHHHHHHHHHCHHHHHHCCCEEECCCCCHHHHCCHHHHHHHHHH
KGLTGLAKQRKVKVVRGTGRFTSPNMIEVETSDGKKTVSFEHCIIAAGSAAARIPGFPYD
HHHHHHHHHCEEEEEECCCCCCCCCEEEEECCCCCEEEEECEEEEECCCCHHCCCCCCCC
DPRIIDSTGALKLESIPKRMLIIGGGIIGLEMATVYDALGSRISVVELMDQLIPGADADL
CCEEECCCCCEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
IRPLHKRIQKRYEAIYLKTKVTRIEALQEGLRVTFEGSSEGGGPEGTGAPEPQVYDRILM
HHHHHHHHHHHHHEEEEHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
AVGRRPNGREIGAEKAGIAVNERGFIPVDKQLRTNVSHIFAIGDIAGEPMLAHKASHEGK
HHCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHCCHHHEEEEECCCCCCHHHHCCCCCCC
LAAEIIAGGEKMKSAAFDARAIPSVAYTDPEIAWMGLTETEAKKQGIEIEKAVFPWAVSG
CHHHHHHCCHHHHHHHCCHHCCCCEEECCCCEEEECCCHHHHHHCCCEEEHEECCEEECC
RALAMARDEGMTKLILDKKTRRILGAGIVGINAGELISETVLGLEMGADMEDIGLTIHPH
CEEEEECCCCCEEEEHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCHHHCCEEECCC
PTLSETVFFAAEIAEGTITDLYMPKK
CCHHHHHEEEEHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]