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The map label for this gene is leuA

Identifier: 82701587

GI number: 82701587

Start: 502976

End: 504508

Strand: Reverse

Name: leuA

Synonym: Nmul_A0453

Alternate gene names: 82701587

Gene position: 504508-502976 (Counterclockwise)

Preceding gene: 82701590

Following gene: 82701586

Centisome position: 15.84

GC content: 56.88

Gene sequence:

>1533_bases
TTGAAAGACCATCTCATCATTTTTGATACCACCTTGCGTGACGGCGAGCAAAGCCCGGGTGCCTCCATGACGAGGGAGGA
GAAAGTGCGGATCGCCCGGCAACTCGAACGAATGCGGGTTGATGTCATTGAAGCGGGTTTTCCAGCTGCCTCGAATGGCG
ATTTCGAAGCGGTAAAAGCCGTAGCCGCTGCGATCAAGGGCAGTACCATATGCGGTCTTGCACGCGCAATCGAAGGCGAT
ATCAGGCGTGCAGGCGAAGCATTGCGGGGAGCCAACTCGGCGCGCATCCACACCTTTATCGCCACCTCGCCCATTCACAT
GGAAAAGAAACTGCGCATGTCCCCGGATCAGGTGGTGGAACAGGCGGTGAAAGCGGTGAAATTTGCGCGTCAATACACCG
ATGACGTCGAATTCTCCCCTGAGGATGCGGGACGCTCTGACATCACCTTTCTTTGCCGCGTGCTCGAAGCGGTCATCGAT
GCCGGAGCGGGAACTGTGAATATTCCCGATACGGTGGGCTATACCATGCCCCAGCAATTCGGGGAACTCATTCGTACCCT
CCGCGAGAGGATTCCCAATTCGGATAAAGTCATATTCTCGGTGCACTGCCATAATGATCTTGGACTGGCAGTGGCCAATT
CGTTATCGGCAGTGATGAACGGAGCACGCCAGGTCGAATGCACGGTAAACGGACTGGGCGAGCGCGCGGGCAATGCCTCC
CTGGAGGAGGTCGTCATGGCTGTGCGCACCCGCCAGGATTTCTTTCCTTGTGATACCCGCATCGATAGTGTCCATATTGT
GCCCGCAAGCAAGCTGGTTTCAGGCATCACCGGGTTTCCGGTACAGCCGAACAAGGCAATTGTGGGCGCAAACGCATTCG
CACACGAATCCGGAATACATCAGGACGGGGTATTGAAACACCGCGAAACATACGAAATCATGCGAGCGGAGGATGTGGGC
TGGGGGGCAAACAAATTGCTGCTGGGCAAGCATTCCGGACGCAACGCCTTCCGCAGCCGCTTGGCAGAGCTCGGGATCGA
GCTGGAATCGGAAGAAACTCTTAATGCCACCTTCATGCGCTTCAAGGAGCTGGCAGACAAGAAGCATGATATTTTCGATG
AAGACCTGCATGCCCTGGTTTCCGATGACGCCGCCGCCTTGCAGGAGCACTACAAGCTCCTGTCACTGACAGCGCACAGC
GAAACGGGGGAAACGCCTCATGCCAGTATAGTAATCGGTGAAGAAGGCAGGGAATCGCGCGCGGAGTCGGATGGAAGCGG
ACCGGTCGACGCCACTTTCCGCGCCATCGAAAAGATACTGAACAGCGGCGTGGAACTGCAGCTCTACTCCGTCAATGCGA
TCACCAGTGGCACTGATTCCCAGGGCGAGGTCACGGTCCGGCTGCAGAAGTCGGGCCGTATCGTCAATGGTAATGGCGCT
GATACGGACATTGTCGTCGCCTCCGCCAAGGCGTATCTCAGCGCTCTGAACAAGCTGCACAGCAAGCTGGAGCGGGCACA
CCCGCAAGTGTAG

Upstream 100 bases:

>100_bases
TACCGATCTCCCTCAAGTCTTTACCAGTTTTTATCGATGGAATCCGTTTCTGGATTCCCTATCAGAGTTCGAGTATTCTT
TCTGAAAAACGGAGAAACAA

Downstream 100 bases:

>100_bases
AAATCTTGAAGAACGCGGCAGGACAAACCGGGGCTGTCTGTCCTGCCTCCAAAGCGGGGCTACGCGTGGAGCTTTTCTCT
CAATCCTCACGACCTGTCGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKDHLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIEAGFPAASNGDFEAVKAVAAAIKGSTICGLARAIEGD
IRRAGEALRGANSARIHTFIATSPIHMEKKLRMSPDQVVEQAVKAVKFARQYTDDVEFSPEDAGRSDITFLCRVLEAVID
AGAGTVNIPDTVGYTMPQQFGELIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLSAVMNGARQVECTVNGLGERAGNAS
LEEVVMAVRTRQDFFPCDTRIDSVHIVPASKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLAELGIELESEETLNATFMRFKELADKKHDIFDEDLHALVSDDAAALQEHYKLLSLTAHS
ETGETPHASIVIGEEGRESRAESDGSGPVDATFRAIEKILNSGVELQLYSVNAITSGTDSQGEVTVRLQKSGRIVNGNGA
DTDIVVASAKAYLSALNKLHSKLERAHPQV

Sequences:

>Translated_510_residues
MKDHLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIEAGFPAASNGDFEAVKAVAAAIKGSTICGLARAIEGD
IRRAGEALRGANSARIHTFIATSPIHMEKKLRMSPDQVVEQAVKAVKFARQYTDDVEFSPEDAGRSDITFLCRVLEAVID
AGAGTVNIPDTVGYTMPQQFGELIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLSAVMNGARQVECTVNGLGERAGNAS
LEEVVMAVRTRQDFFPCDTRIDSVHIVPASKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLAELGIELESEETLNATFMRFKELADKKHDIFDEDLHALVSDDAAALQEHYKLLSLTAHS
ETGETPHASIVIGEEGRESRAESDGSGPVDATFRAIEKILNSGVELQLYSVNAITSGTDSQGEVTVRLQKSGRIVNGNGA
DTDIVVASAKAYLSALNKLHSKLERAHPQV
>Mature_510_residues
MKDHLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIEAGFPAASNGDFEAVKAVAAAIKGSTICGLARAIEGD
IRRAGEALRGANSARIHTFIATSPIHMEKKLRMSPDQVVEQAVKAVKFARQYTDDVEFSPEDAGRSDITFLCRVLEAVID
AGAGTVNIPDTVGYTMPQQFGELIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLSAVMNGARQVECTVNGLGERAGNAS
LEEVVMAVRTRQDFFPCDTRIDSVHIVPASKLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WGANKLLLGKHSGRNAFRSRLAELGIELESEETLNATFMRFKELADKKHDIFDEDLHALVSDDAAALQEHYKLLSLTAHS
ETGETPHASIVIGEEGRESRAESDGSGPVDATFRAIEKILNSGVELQLYSVNAITSGTDSQGEVTVRLQKSGRIVNGNGA
DTDIVVASAKAYLSALNKLHSKLERAHPQV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=51.6966067864271, Blast_Score=480, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6324682, Length=556, Percent_Identity=27.6978417266187, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=29.5811518324607, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=29.3628808864266, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=548, Percent_Identity=27.1897810218978, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_NITMU (Q2YBW0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411153.1
- ProteinModelPortal:   Q2YBW0
- SMR:   Q2YBW0
- STRING:   Q2YBW0
- GeneID:   3786000
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0453
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q2YBW0
- ProtClustDB:   PRK00915
- BioCyc:   NMUL323848:NMUL_A0453-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55311; Mature: 55311

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDHLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIEAGFPAASNGDFEAVKA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VAAAIKGSTICGLARAIEGDIRRAGEALRGANSARIHTFIATSPIHMEKKLRMSPDQVVE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCHHHHHH
QAVKAVKFARQYTDDVEFSPEDAGRSDITFLCRVLEAVIDAGAGTVNIPDTVGYTMPQQF
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
GELIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLSAVMNGARQVECTVNGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCHHCCCCCCC
LEEVVMAVRTRQDFFPCDTRIDSVHIVPASKLVSGITGFPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLLLGKHSGRNAFRSRLAELGIELESEETLNATFMR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
FKELADKKHDIFDEDLHALVSDDAAALQEHYKLLSLTAHSETGETPHASIVIGEEGRESR
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCHHH
AESDGSGPVDATFRAIEKILNSGVELQLYSVNAITSGTDSQGEVTVRLQKSGRIVNGNGA
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCEEECCCCC
DTDIVVASAKAYLSALNKLHSKLERAHPQV
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDHLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIEAGFPAASNGDFEAVKA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VAAAIKGSTICGLARAIEGDIRRAGEALRGANSARIHTFIATSPIHMEKKLRMSPDQVVE
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCHHHHHH
QAVKAVKFARQYTDDVEFSPEDAGRSDITFLCRVLEAVIDAGAGTVNIPDTVGYTMPQQF
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
GELIRTLRERIPNSDKVIFSVHCHNDLGLAVANSLSAVMNGARQVECTVNGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCHHCCCCCCC
LEEVVMAVRTRQDFFPCDTRIDSVHIVPASKLVSGITGFPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWGANKLLLGKHSGRNAFRSRLAELGIELESEETLNATFMR
CCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
FKELADKKHDIFDEDLHALVSDDAAALQEHYKLLSLTAHSETGETPHASIVIGEEGRESR
HHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCHHH
AESDGSGPVDATFRAIEKILNSGVELQLYSVNAITSGTDSQGEVTVRLQKSGRIVNGNGA
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEEECCCEEECCCCC
DTDIVVASAKAYLSALNKLHSKLERAHPQV
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA