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The map label for this gene is pykA [H]

Identifier: 82701519

GI number: 82701519

Start: 423112

End: 424566

Strand: Reverse

Name: pykA [H]

Synonym: Nmul_A0385

Alternate gene names: 82701519

Gene position: 424566-423112 (Counterclockwise)

Preceding gene: 82701520

Following gene: 82701518

Centisome position: 13.33

GC content: 56.36

Gene sequence:

>1455_bases
ATGATTCGAAGAACAAAAATCGTAGCAACACTTGGTCCCGCATCCCGAGATGCAAAGGTGCTGGAGCGAATGATAAACGC
CGGCGTTGATGTAGTACGCATCAACTTTTCCCATGGGACCAGGGAAGAGCATATCGAAGGCGCCGAGTTGGCGCGCTCCC
TCGCACGTGCCGCTGGCCACGCAGTAGGTGTGCTGGCGGACTTGCAGGGACCCAAGATTCGAATCGGCAAATTTGAAAAT
AATAAAATTCTGCTGAAAACGGGCGATCAATTCATACTCGACGCCGAATGCGAGCTTGGAAACCAGGAACGGGTGGGCCT
GGATTACAAGGAACTGCCCAATGATGTCGAGACCGGTGCAACACTGATCCTGGATGATGGACGCATCGTGCTCGGCGTTT
CCGAAGTCAAGGGAAGCCAGGTTTATTGCGTGGTTGAGCAAGGTGGGATACTGTCCAACAACAAGGGAATCAACCGCAAA
GGCGGCGGCCTGAGTGCTCCTGCCCTCACCGGCAAGGACGTGGAAGATATCCGAACGGCCGCAGCGCTTAAAGCCGACTA
TCTGGCGGTATCATTTCCCCGTTCTGGCGATGATATGCGCTGGGCACGCGATGTGATGCGCGAGGCGGGCGGAAAAAGCT
TATTGATGGCGAAGATCGAACGATCGGAAGCCATCCTGGCGCTGGATGACATTCTGGAGGCTTCCGATGCGATCATGGTT
GCGCGCGGAGACCTTGCGGTAGAGGTAGGCGATGCAGCGGTGCCGGCATTGCAAAAACGAATGATTCGCACTGCCCGAAC
CAAGAACAAGCTGGTGGTAACAGCTACCCAGATGATGGAGTCCATGGTCAGCAGTCCCATCCCTACCCGCGCGGAGGTAT
CGGATGTAGCAAATGCGGTATTGGATGGCACTGATGGGGTGATGCTGTCAGCGGAATCGGCCACCGGCCAATATCCCGTT
GAAGCGGTTGCGGCGATGGCGAGGGTATGCCTGGAGGCGGAAAAGGAATACCTGGTAAGCAGCGAAAACCGACGGCTGCA
GGGCGGCCTGCCCGACAATATCGAGGAAGCAATTGCCCGTGCGACCATGTTCACCGCAGGAGGATTGAAGATCAGGGCCA
TTGCGGCGCTTACACAAAGCGGCAGGACCGTGTTGCTGATGTCGCGCAGGAGTTCCACCGTACCCATATTCGCGCTGAGT
CCCCAGGAAGATACACGGCGCAAGATGACGTTATTTCGGGGTGTGTACCCCGTCAAGTTCGGCGGAGGCTCGAACGACCC
GGAGATCATCCTGAATCATGCCGAAAACGAGTTGCTCAGACGAGGCGCGGTACGCAACGGCGACCTAATGATCATGACCA
TCGGTGAACCGGTGGGCAAGGCAGGCGGCACCAATACGATGAAAATAGTGAAGGTCGGCGAGCACAGAAAACAGGAACGG
AAGGTGGAATCCTGA

Upstream 100 bases:

>100_bases
CTGCCGTGGAAATACTCGAACAGCGCGCAGCCGGCAGCAGATAGGTCCAGGTAGCGAATATGCGGCTTGATATGCCGGTT
CAACGCGTGCCCTCATCAGA

Downstream 100 bases:

>100_bases
GCAGTGCAGATATTCGTCTCGATCTTTTCACTGCCTGTGATGTTTTACCATATACACTGATTCTAACTTTGAGGAGGTTC
TAATGGCACTCGTATCATTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MIRRTKIVATLGPASRDAKVLERMINAGVDVVRINFSHGTREEHIEGAELARSLARAAGHAVGVLADLQGPKIRIGKFEN
NKILLKTGDQFILDAECELGNQERVGLDYKELPNDVETGATLILDDGRIVLGVSEVKGSQVYCVVEQGGILSNNKGINRK
GGGLSAPALTGKDVEDIRTAAALKADYLAVSFPRSGDDMRWARDVMREAGGKSLLMAKIERSEAILALDDILEASDAIMV
ARGDLAVEVGDAAVPALQKRMIRTARTKNKLVVTATQMMESMVSSPIPTRAEVSDVANAVLDGTDGVMLSAESATGQYPV
EAVAAMARVCLEAEKEYLVSSENRRLQGGLPDNIEEAIARATMFTAGGLKIRAIAALTQSGRTVLLMSRRSSTVPIFALS
PQEDTRRKMTLFRGVYPVKFGGGSNDPEIILNHAENELLRRGAVRNGDLMIMTIGEPVGKAGGTNTMKIVKVGEHRKQER
KVES

Sequences:

>Translated_484_residues
MIRRTKIVATLGPASRDAKVLERMINAGVDVVRINFSHGTREEHIEGAELARSLARAAGHAVGVLADLQGPKIRIGKFEN
NKILLKTGDQFILDAECELGNQERVGLDYKELPNDVETGATLILDDGRIVLGVSEVKGSQVYCVVEQGGILSNNKGINRK
GGGLSAPALTGKDVEDIRTAAALKADYLAVSFPRSGDDMRWARDVMREAGGKSLLMAKIERSEAILALDDILEASDAIMV
ARGDLAVEVGDAAVPALQKRMIRTARTKNKLVVTATQMMESMVSSPIPTRAEVSDVANAVLDGTDGVMLSAESATGQYPV
EAVAAMARVCLEAEKEYLVSSENRRLQGGLPDNIEEAIARATMFTAGGLKIRAIAALTQSGRTVLLMSRRSSTVPIFALS
PQEDTRRKMTLFRGVYPVKFGGGSNDPEIILNHAENELLRRGAVRNGDLMIMTIGEPVGKAGGTNTMKIVKVGEHRKQER
KVES
>Mature_484_residues
MIRRTKIVATLGPASRDAKVLERMINAGVDVVRINFSHGTREEHIEGAELARSLARAAGHAVGVLADLQGPKIRIGKFEN
NKILLKTGDQFILDAECELGNQERVGLDYKELPNDVETGATLILDDGRIVLGVSEVKGSQVYCVVEQGGILSNNKGINRK
GGGLSAPALTGKDVEDIRTAAALKADYLAVSFPRSGDDMRWARDVMREAGGKSLLMAKIERSEAILALDDILEASDAIMV
ARGDLAVEVGDAAVPALQKRMIRTARTKNKLVVTATQMMESMVSSPIPTRAEVSDVANAVLDGTDGVMLSAESATGQYPV
EAVAAMARVCLEAEKEYLVSSENRRLQGGLPDNIEEAIARATMFTAGGLKIRAIAALTQSGRTVLLMSRRSSTVPIFALS
PQEDTRRKMTLFRGVYPVKFGGGSNDPEIILNHAENELLRRGAVRNGDLMIMTIGEPVGKAGGTNTMKIVKVGEHRKQER
KVES

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=37.4233128834356, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.9168356997972, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.9168356997972, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=35.5691056910569, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=35.5691056910569, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.5853658536585, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.5853658536585, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.0425531914894, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.0425531914894, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.7102803738318, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=53.6687631027254, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787965, Length=479, Percent_Identity=37.3695198329854, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=37.9166666666667, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=36.1963190184049, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=36.1963190184049, Blast_Score=257, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=36.1963190184049, Blast_Score=257, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=36.1963190184049, Blast_Score=257, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=32.9243353783231, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=493, Percent_Identity=31.8458417849899, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=36.40081799591, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=36.40081799591, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648966, Length=405, Percent_Identity=38.5185185185185, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=31.8930041152263, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24646914, Length=269, Percent_Identity=33.8289962825279, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52025; Mature: 52025

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRTKIVATLGPASRDAKVLERMINAGVDVVRINFSHGTREEHIEGAELARSLARAAGH
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHCCC
AVGVLADLQGPKIRIGKFENNKILLKTGDQFILDAECELGNQERVGLDYKELPNDVETGA
CEEEEEECCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHCCCCHHCCC
TLILDDGRIVLGVSEVKGSQVYCVVEQGGILSNNKGINRKGGGLSAPALTGKDVEDIRTA
EEEEECCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AALKADYLAVSFPRSGDDMRWARDVMREAGGKSLLMAKIERSEAILALDDILEASDAIMV
HHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHCCCCEEEE
ARGDLAVEVGDAAVPALQKRMIRTARTKNKLVVTATQMMESMVSSPIPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDGVMLSAESATGQYPVEAVAAMARVCLEAEKEYLVSSENRRLQGGLPDNIEEAIAR
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHH
ATMFTAGGLKIRAIAALTQSGRTVLLMSRRSSTVPIFALSPQEDTRRKMTLFRGVYPVKF
HHHEECCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCEEEE
GGGSNDPEIILNHAENELLRRGAVRNGDLMIMTIGEPVGKAGGTNTMKIVKVGEHRKQER
CCCCCCCCEEEECHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHHHH
KVES
CCCC
>Mature Secondary Structure
MIRRTKIVATLGPASRDAKVLERMINAGVDVVRINFSHGTREEHIEGAELARSLARAAGH
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHCCC
AVGVLADLQGPKIRIGKFENNKILLKTGDQFILDAECELGNQERVGLDYKELPNDVETGA
CEEEEEECCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCCHHHCCCCHHCCC
TLILDDGRIVLGVSEVKGSQVYCVVEQGGILSNNKGINRKGGGLSAPALTGKDVEDIRTA
EEEEECCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHH
AALKADYLAVSFPRSGDDMRWARDVMREAGGKSLLMAKIERSEAILALDDILEASDAIMV
HHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHHHHHCCCCEEEE
ARGDLAVEVGDAAVPALQKRMIRTARTKNKLVVTATQMMESMVSSPIPTRAEVSDVANAV
EECCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHH
LDGTDGVMLSAESATGQYPVEAVAAMARVCLEAEKEYLVSSENRRLQGGLPDNIEEAIAR
HCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHH
ATMFTAGGLKIRAIAALTQSGRTVLLMSRRSSTVPIFALSPQEDTRRKMTLFRGVYPVKF
HHHEECCCEEEEEEEEECCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCEEEE
GGGSNDPEIILNHAENELLRRGAVRNGDLMIMTIGEPVGKAGGTNTMKIVKVGEHRKQER
CCCCCCCCEEEECHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHHHH
KVES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]