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The map label for this gene is pgk

Identifier: 82701520

GI number: 82701520

Start: 424623

End: 425810

Strand: Reverse

Name: pgk

Synonym: Nmul_A0386

Alternate gene names: 82701520

Gene position: 425810-424623 (Counterclockwise)

Preceding gene: 82701521

Following gene: 82701519

Centisome position: 13.37

GC content: 53.54

Gene sequence:

>1188_bases
GTGTCTGTCATTAGAGTCACCGACCTTGATCTGAAAGGCAAGCGCGTGTTCATCCGCGCCGACCTCAATGTGCCCGTGAA
AGATGGCAAAGTTACCTCCGACGCACGCATCAGCGCCTCCATGGGTACCATCGACTACTGCCTGAAGCAAGGCGGCAAAG
TAATGGTTACCTCCCATCTGGGACGACCGGAAGAGGGCGTATGGTCAGAAGAAAATTCGCTCAAACCGGTAGCCGACAAT
ATTTCCGAACGTCTCGGCAAGCCGGCGCGCCTGATCAAGGATTGGGTGGACGGCAACTTCGACGTGGCCGAAGGCGAACT
GGTCATCCTCGAAAATTGCCGCTTCAATAAGGGGGAAAAGAAAAATGCAGATGAAACGGCCCAGAAATATGCAAAGCTGT
GCGATGTATTTGTGATGGATGCGTTTGGCACAGCGCATCGGGCCGAAGCATCGACCTATGGCATTGCCAAATACGCTCCT
GTTGCCTGCGCGGGCATTCTGCTTACAGAAGAGCTGGACGCGCTGACCAAGGCGTTGTTGAATCCAGCCCGCCCCATGGT
GGCGATCGTCGGAGGCTCGAAGGTCTCGACAAAATTGACCGTTCTGGAATCCCTGTCAGAAAAAGTGGATCAACTGGTGG
TGGGGGGCGGCATCGCCAATACCTTCCTCAAAGCGGCGGGACAGAACGTTGGAAAATCCCTGTGCGAAGATGATCTGGTT
CCCATCGCCAAAATGCTCATGGAAAAAATGGCAAAGCGGAATGCGACCATTCCAATTGCCGTTGACGTTGTCGTCGGGAA
AAAATTCGACGCCAGTGAACCGGCGGTATTGAAGAATGCAGATGCGGTAACAGATGACGATATGATATTCGATATCGGCC
CGAAGAGTGCCCGCGAGTTGTCGGACATCATCATGAAAGCCGGTACCGTAGTATGGAACGGCCCCGTCGGAGTGTTCGAG
TTCGATCAATTCGGTGAAGGCACCAAAACCATTGCCATGGCTATCGCCGATACCGATGCCTTTACCCTGGCAGGGGGGGG
AGATACCATTGCCGCTATCCAGAAATACGATATTTATGACAGGGTTTCTTACATTTCCACTGCGGGGGGAGCCTTTCTGG
AATTTCTGGAAGGCAAGAAGTTACCTGCCGTGGAAATACTCGAACAGCGCGCAGCCGGCAGCAGATAG

Upstream 100 bases:

>100_bases
ACTCGGAATCGTTTTTCTTCCCCCTGATCCCTGTCCTTCTCTTCAATTCATCATAAGGAATCCCTAGACTTCCTTATGTC
TTAAAACGTGGAGTTCCCCA

Downstream 100 bases:

>100_bases
GTCCAGGTAGCGAATATGCGGCTTGATATGCCGGTTCAACGCGTGCCCTCATCAGAATGATTCGAAGAACAAAAATCGTA
GCAACACTTGGTCCCGCATC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSVIRVTDLDLKGKRVFIRADLNVPVKDGKVTSDARISASMGTIDYCLKQGGKVMVTSHLGRPEEGVWSEENSLKPVADN
ISERLGKPARLIKDWVDGNFDVAEGELVILENCRFNKGEKKNADETAQKYAKLCDVFVMDAFGTAHRAEASTYGIAKYAP
VACAGILLTEELDALTKALLNPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGIANTFLKAAGQNVGKSLCEDDLV
PIAKMLMEKMAKRNATIPIAVDVVVGKKFDASEPAVLKNADAVTDDDMIFDIGPKSARELSDIIMKAGTVVWNGPVGVFE
FDQFGEGTKTIAMAIADTDAFTLAGGGDTIAAIQKYDIYDRVSYISTAGGAFLEFLEGKKLPAVEILEQRAAGSR

Sequences:

>Translated_395_residues
MSVIRVTDLDLKGKRVFIRADLNVPVKDGKVTSDARISASMGTIDYCLKQGGKVMVTSHLGRPEEGVWSEENSLKPVADN
ISERLGKPARLIKDWVDGNFDVAEGELVILENCRFNKGEKKNADETAQKYAKLCDVFVMDAFGTAHRAEASTYGIAKYAP
VACAGILLTEELDALTKALLNPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGIANTFLKAAGQNVGKSLCEDDLV
PIAKMLMEKMAKRNATIPIAVDVVVGKKFDASEPAVLKNADAVTDDDMIFDIGPKSARELSDIIMKAGTVVWNGPVGVFE
FDQFGEGTKTIAMAIADTDAFTLAGGGDTIAAIQKYDIYDRVSYISTAGGAFLEFLEGKKLPAVEILEQRAAGSR
>Mature_394_residues
SVIRVTDLDLKGKRVFIRADLNVPVKDGKVTSDARISASMGTIDYCLKQGGKVMVTSHLGRPEEGVWSEENSLKPVADNI
SERLGKPARLIKDWVDGNFDVAEGELVILENCRFNKGEKKNADETAQKYAKLCDVFVMDAFGTAHRAEASTYGIAKYAPV
ACAGILLTEELDALTKALLNPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGIANTFLKAAGQNVGKSLCEDDLVP
IAKMLMEKMAKRNATIPIAVDVVVGKKFDASEPAVLKNADAVTDDDMIFDIGPKSARELSDIIMKAGTVVWNGPVGVFEF
DQFGEGTKTIAMAIADTDAFTLAGGGDTIAAIQKYDIYDRVSYISTAGGAFLEFLEGKKLPAVEILEQRAAGSR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=39.8034398034398, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=38.5749385749386, Blast_Score=266, Evalue=3e-71,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=65.4731457800512, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=41.304347826087, Blast_Score=290, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=39.618138424821, Blast_Score=270, Evalue=4e-73,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=39.5631067961165, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=37.378640776699, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_NITMU (Q2YC27)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411086.1
- ProteinModelPortal:   Q2YC27
- SMR:   Q2YC27
- STRING:   Q2YC27
- GeneID:   3784081
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0386
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   CEESMID
- PhylomeDB:   Q2YC27
- ProtClustDB:   PRK00073
- BioCyc:   NMUL323848:NMUL_A0386-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42273; Mature: 42142

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 198-198 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIRVTDLDLKGKRVFIRADLNVPVKDGKVTSDARISASMGTIDYCLKQGGKVMVTSHL
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPEEGVWSEENSLKPVADNISERLGKPARLIKDWVDGNFDVAEGELVILENCRFNKGEK
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCC
KNADETAQKYAKLCDVFVMDAFGTAHRAEASTYGIAKYAPVACAGILLTEELDALTKALL
CCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGIANTFLKAAGQNVGKSLCEDDLV
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCHHHHHHHHHHHHH
PIAKMLMEKMAKRNATIPIAVDVVVGKKFDASEPAVLKNADAVTDDDMIFDIGPKSAREL
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHH
SDIIMKAGTVVWNGPVGVFEFDQFGEGTKTIAMAIADTDAFTLAGGGDTIAAIQKYDIYD
HHHHHHCCCEEECCCCCEEECHHCCCCCEEEEEEEECCCEEEEECCCCEEEHHHHHHHHH
RVSYISTAGGAFLEFLEGKKLPAVEILEQRAAGSR
HHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SVIRVTDLDLKGKRVFIRADLNVPVKDGKVTSDARISASMGTIDYCLKQGGKVMVTSHL
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPEEGVWSEENSLKPVADNISERLGKPARLIKDWVDGNFDVAEGELVILENCRFNKGEK
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCC
KNADETAQKYAKLCDVFVMDAFGTAHRAEASTYGIAKYAPVACAGILLTEELDALTKALL
CCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
NPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGIANTFLKAAGQNVGKSLCEDDLV
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCHHHHHHHHHHHHH
PIAKMLMEKMAKRNATIPIAVDVVVGKKFDASEPAVLKNADAVTDDDMIFDIGPKSAREL
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCEEEECCCHHHHHH
SDIIMKAGTVVWNGPVGVFEFDQFGEGTKTIAMAIADTDAFTLAGGGDTIAAIQKYDIYD
HHHHHHCCCEEECCCCCEEECHHCCCCCEEEEEEEECCCEEEEECCCCEEEHHHHHHHHH
RVSYISTAGGAFLEFLEGKKLPAVEILEQRAAGSR
HHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA