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The map label for this gene is pyrC [H]

Identifier: 82701488

GI number: 82701488

Start: 383760

End: 384803

Strand: Reverse

Name: pyrC [H]

Synonym: Nmul_A0354

Alternate gene names: 82701488

Gene position: 384803-383760 (Counterclockwise)

Preceding gene: 82701489

Following gene: 82701480

Centisome position: 12.08

GC content: 57.66

Gene sequence:

>1044_bases
TTGAATACCTTCACACTTACACGCCCGGACGACTTCCACCTGCATCTCCGCGATGGCGAACACATGCGGGCAGTGCTGGC
GGACACTGCCCGCCGTTTTGCCCGCGCAATCGTCATGCCCAACCTCAAGCCTCCCGTCATCACCACGGAAATGGCGGTGG
CGTATCGCGGGCGGCTTCTTGCCGCCCTGCCGCAGGGCGTGCGCTTCGAGCCGCTGATGACCCTCTACCTGACCGATAAC
ACCTCCCCTTCGGAAATCATCGAAGCAAAAAGAAGCGGAGTAATCTACGGCATCAAATACTATCCCGCAGGCGCTACCAC
AAACTCCGCGGCAGGAGTCACCGACATTGCCAGATGCCATGAAACTCTGGAAGCCATGGAGCAAGCGGAAATGCCCATGC
TGGTGCATGGCGAGGTAACCGATCCGGAGGTGGATGTATTCGACAGGGAAAAGGTCTTTCTTGAACGGATGCTGATCCCG
TTGACCCAGCGTTTTCCGCGATTGCGCGTGGTATTCGAGCATATCACCACACGGGAAGCGGTAGAGTTCGTCATCAACGC
GCCGAAAACCGTCGCCGCCACCATCACGGCCCACCACCTCCTGATCAGCCGGAATGCCCTTTTTCAAGGGGGTATTCGGC
CTCACCACTATTGCCTGCCCATCCTCAAGCGGGAAACCCACCGGCAGAAGCTGATCGAAGCGGCCACCAGCGGCAATCCG
AAATTTTTCCTCGGCACCGATAGCGCTCCCCACGCACAATTCGCCAAGGAAAACGCCTGCGGCTGCGCCGGCATCTATAC
TGCTCACGCAGCGATCGAGCTGTATGCGGAAGCATTCGAACAGGCGGGCGCGCTGGAAAAACTGGAAGCCTTTGCCAGTT
TTCATGGTGCGGATTTCTATCAGCTACCGCGCAATCAGGACAAGATTACGTTGAAGAAGGAAAATTGGAGGGTGCCGGCG
CAGCTGGAATTTGGCGGCGAAAGCCTGATTCCATTCCGGGCGGGGGAAAACGTTACCTGGGCCTTGGATAAAACCGGGAA
TTAG

Upstream 100 bases:

>100_bases
ATTAATCGGGGAATAGCAGGCTCGAGTCCTCCTGAGTCCTCAATGGGAGGCTCATGGTATTTTTCGTGCCAGTTAAATAA
AAAACAGTGCCGCAGCCATC

Downstream 100 bases:

>100_bases
CGGCGAAACGGAATTTTCACCAAACTTTCCTGATCAGAAAATAGCTGCGGGAATTGTTTCCCGGTCACTCGCGCCCGGGA
GTGCCTACCAGTGTTTTTTG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNTFTLTRPDDFHLHLRDGEHMRAVLADTARRFARAIVMPNLKPPVITTEMAVAYRGRLLAALPQGVRFEPLMTLYLTDN
TSPSEIIEAKRSGVIYGIKYYPAGATTNSAAGVTDIARCHETLEAMEQAEMPMLVHGEVTDPEVDVFDREKVFLERMLIP
LTQRFPRLRVVFEHITTREAVEFVINAPKTVAATITAHHLLISRNALFQGGIRPHHYCLPILKRETHRQKLIEAATSGNP
KFFLGTDSAPHAQFAKENACGCAGIYTAHAAIELYAEAFEQAGALEKLEAFASFHGADFYQLPRNQDKITLKKENWRVPA
QLEFGGESLIPFRAGENVTWALDKTGN

Sequences:

>Translated_347_residues
MNTFTLTRPDDFHLHLRDGEHMRAVLADTARRFARAIVMPNLKPPVITTEMAVAYRGRLLAALPQGVRFEPLMTLYLTDN
TSPSEIIEAKRSGVIYGIKYYPAGATTNSAAGVTDIARCHETLEAMEQAEMPMLVHGEVTDPEVDVFDREKVFLERMLIP
LTQRFPRLRVVFEHITTREAVEFVINAPKTVAATITAHHLLISRNALFQGGIRPHHYCLPILKRETHRQKLIEAATSGNP
KFFLGTDSAPHAQFAKENACGCAGIYTAHAAIELYAEAFEQAGALEKLEAFASFHGADFYQLPRNQDKITLKKENWRVPA
QLEFGGESLIPFRAGENVTWALDKTGN
>Mature_347_residues
MNTFTLTRPDDFHLHLRDGEHMRAVLADTARRFARAIVMPNLKPPVITTEMAVAYRGRLLAALPQGVRFEPLMTLYLTDN
TSPSEIIEAKRSGVIYGIKYYPAGATTNSAAGVTDIARCHETLEAMEQAEMPMLVHGEVTDPEVDVFDREKVFLERMLIP
LTQRFPRLRVVFEHITTREAVEFVINAPKTVAATITAHHLLISRNALFQGGIRPHHYCLPILKRETHRQKLIEAATSGNP
KFFLGTDSAPHAQFAKENACGCAGIYTAHAAIELYAEAFEQAGALEKLEAFASFHGADFYQLPRNQDKITLKKENWRVPA
QLEFGGESLIPFRAGENVTWALDKTGN

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=339, Percent_Identity=50.7374631268437, Blast_Score=351, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6323452, Length=357, Percent_Identity=34.453781512605, Blast_Score=197, Evalue=3e-51,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 38782; Mature: 38782

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFTLTRPDDFHLHLRDGEHMRAVLADTARRFARAIVMPNLKPPVITTEMAVAYRGRLL
CCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCHH
AALPQGVRFEPLMTLYLTDNTSPSEIIEAKRSGVIYGIKYYPAGATTNSAAGVTDIARCH
HHCCCCCEECEEEEEEEECCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHHHHHHH
ETLEAMEQAEMPMLVHGEVTDPEVDVFDREKVFLERMLIPLTQRFPRLRVVFEHITTREA
HHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VEFVINAPKTVAATITAHHLLISRNALFQGGIRPHHYCLPILKRETHRQKLIEAATSGNP
HHHHHCCCCCEEHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
KFFLGTDSAPHAQFAKENACGCAGIYTAHAAIELYAEAFEQAGALEKLEAFASFHGADFY
EEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
QLPRNQDKITLKKENWRVPAQLEFGGESLIPFRAGENVTWALDKTGN
ECCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MNTFTLTRPDDFHLHLRDGEHMRAVLADTARRFARAIVMPNLKPPVITTEMAVAYRGRLL
CCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCHH
AALPQGVRFEPLMTLYLTDNTSPSEIIEAKRSGVIYGIKYYPAGATTNSAAGVTDIARCH
HHCCCCCEECEEEEEEEECCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHHHHHHH
ETLEAMEQAEMPMLVHGEVTDPEVDVFDREKVFLERMLIPLTQRFPRLRVVFEHITTREA
HHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
VEFVINAPKTVAATITAHHLLISRNALFQGGIRPHHYCLPILKRETHRQKLIEAATSGNP
HHHHHCCCCCEEHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
KFFLGTDSAPHAQFAKENACGCAGIYTAHAAIELYAEAFEQAGALEKLEAFASFHGADFY
EEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
QLPRNQDKITLKKENWRVPAQLEFGGESLIPFRAGENVTWALDKTGN
ECCCCCCEEEEECCCCCCCEEEECCCCEEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]