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The map label for this gene is camA [H]

Identifier: 82701489

GI number: 82701489

Start: 384991

End: 386169

Strand: Reverse

Name: camA [H]

Synonym: Nmul_A0355

Alternate gene names: 82701489

Gene position: 386169-384991 (Counterclockwise)

Preceding gene: 82701499

Following gene: 82701488

Centisome position: 12.13

GC content: 53.6

Gene sequence:

>1179_bases
ATGAAACACCATAAATACCTTATTGTCGGGGGCGGAATGACCGCCGATTCTGCTGTACATGGTATCCGTAAGATCGACCC
CGATGGCGCAATCGCCGTCATTGGAGAGGAGTTGCATCGTCCTTACAACCGTCCGCCCCTTTCCAAGGCGCTATGGAAGG
ACTCACCCTATGATTCGATCTGGCGAAGCGAGCACGGCCTGAACGTTGCCATGCACCTCGGAAAAAAAATAGTAGCCCTA
GATCCCGCTAACAAGACGGCCACGGATAATGCAGGCAACATCTATACCTATGAAAAACTTTTGCTTGCCACGGGAGGCGA
GGTACGCCGCTTTCCCCATTTCGACAGCGGGATAATCTATTACCGGACGGCGGACGACTACCTTAAGCTGCGGGAATTGA
GTAGTCAGGGTTCGGATTTTGTCGTGATCGGCGGTGGCTTCATCGGTTCGGAAATCGCTGCCGCGCTGGCCATGAATGAT
AAACGGGTCACCATGATTTTTCCGGAAAACGGAATCAGCTCGCGTATTTATCCGCGGCCCCTCGTCGAATTCCTGAATTC
CTACTATCGCGAAAAGGGTGTAATCGTGCTCGCCCCCGAAACTGTCACTTCGATCAGGACGGATGGGACAAAAAAAATTG
TTACCACGGGGAGTGGAACCGAGATTTCCGCCGATGGAGTAGTGGCAGGACTGGGTATTCTTCCCAACACGGAACTGGCT
GTCCAGGCCGGGCTCGCGATTGACAACGGCATCGTAGTGGATGAATTCCTGCGCACCAGCAATCCCGATATTTATGCCGC
CGGCGATGTCGCCAATTTCTACAGTCCCTTGCTCGACAAGCGCATGCGGGTTGAGCATGAAGACAACGCAAACATGATGG
GAGAAGCGGCGGGACGGAACATGGCAGGGAGCCTGGAGCCCTATCATCACCAGCCCTTCTTTTATTCCGACCTGTTTGAT
CTGGGCTATGAAGCAGTCGGTGAACTCGATTCCAGTCTCGATATTGTCGAAGATTGGGTAGAGCCCTTCCGCAAAGGCGT
GATTTATTACCTGCGCGACGAGCTCGTACGCGGTGTACTGCTCTGGAATACCTGGGGACAGGTGGATGCCGCAACCGCGT
TGATTGCCGAAAAGAAACCCTGCACGAACGAGACATTGCTAGGCCGCATCAAGGACTAA

Upstream 100 bases:

>100_bases
TTCCGCACTCTATCGCGCAACAGGTCACACAAAAGGTTTCGGAACATGACAACGGGTCAATTTTTAACCTGCGCTTCATA
TACCTGCAAAGGAAACCAGC

Downstream 100 bases:

>100_bases
AACAGGAGACAGTAGTGCAGGATGGGGGTAAGCTAGATTTGCTGCGATGCCGGATGCTTCGCTTGCCTCTCCTGCAATTT
CCGATGAATTAATCGGGGAA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKHHKYLIVGGGMTADSAVHGIRKIDPDGAIAVIGEELHRPYNRPPLSKALWKDSPYDSIWRSEHGLNVAMHLGKKIVAL
DPANKTATDNAGNIYTYEKLLLATGGEVRRFPHFDSGIIYYRTADDYLKLRELSSQGSDFVVIGGGFIGSEIAAALAMND
KRVTMIFPENGISSRIYPRPLVEFLNSYYREKGVIVLAPETVTSIRTDGTKKIVTTGSGTEISADGVVAGLGILPNTELA
VQAGLAIDNGIVVDEFLRTSNPDIYAAGDVANFYSPLLDKRMRVEHEDNANMMGEAAGRNMAGSLEPYHHQPFFYSDLFD
LGYEAVGELDSSLDIVEDWVEPFRKGVIYYLRDELVRGVLLWNTWGQVDAATALIAEKKPCTNETLLGRIKD

Sequences:

>Translated_392_residues
MKHHKYLIVGGGMTADSAVHGIRKIDPDGAIAVIGEELHRPYNRPPLSKALWKDSPYDSIWRSEHGLNVAMHLGKKIVAL
DPANKTATDNAGNIYTYEKLLLATGGEVRRFPHFDSGIIYYRTADDYLKLRELSSQGSDFVVIGGGFIGSEIAAALAMND
KRVTMIFPENGISSRIYPRPLVEFLNSYYREKGVIVLAPETVTSIRTDGTKKIVTTGSGTEISADGVVAGLGILPNTELA
VQAGLAIDNGIVVDEFLRTSNPDIYAAGDVANFYSPLLDKRMRVEHEDNANMMGEAAGRNMAGSLEPYHHQPFFYSDLFD
LGYEAVGELDSSLDIVEDWVEPFRKGVIYYLRDELVRGVLLWNTWGQVDAATALIAEKKPCTNETLLGRIKD
>Mature_392_residues
MKHHKYLIVGGGMTADSAVHGIRKIDPDGAIAVIGEELHRPYNRPPLSKALWKDSPYDSIWRSEHGLNVAMHLGKKIVAL
DPANKTATDNAGNIYTYEKLLLATGGEVRRFPHFDSGIIYYRTADDYLKLRELSSQGSDFVVIGGGFIGSEIAAALAMND
KRVTMIFPENGISSRIYPRPLVEFLNSYYREKGVIVLAPETVTSIRTDGTKKIVTTGSGTEISADGVVAGLGILPNTELA
VQAGLAIDNGIVVDEFLRTSNPDIYAAGDVANFYSPLLDKRMRVEHEDNANMMGEAAGRNMAGSLEPYHHQPFFYSDLFD
LGYEAVGELDSSLDIVEDWVEPFRKGVIYYLRDELVRGVLLWNTWGQVDAATALIAEKKPCTNETLLGRIKD

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4757732, Length=466, Percent_Identity=32.8326180257511, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI22202629, Length=466, Percent_Identity=32.618025751073, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI22202631, Length=267, Percent_Identity=32.9588014981273, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI195927004, Length=240, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI65787454, Length=338, Percent_Identity=26.6272189349112, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI21389617, Length=338, Percent_Identity=26.6272189349112, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI226437568, Length=338, Percent_Identity=26.6272189349112, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI195927006, Length=193, Percent_Identity=33.6787564766839, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1788892, Length=374, Percent_Identity=32.3529411764706, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=30.1075268817204, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32564386, Length=438, Percent_Identity=29.2237442922374, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17559934, Length=337, Percent_Identity=29.3768545994065, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573993, Length=473, Percent_Identity=30.0211416490486, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24581020, Length=473, Percent_Identity=30.0211416490486, Blast_Score=205, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24639257, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281359715, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281359713, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24639250, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18543267, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24639252, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585130, Length=345, Percent_Identity=23.4782608695652, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43157; Mature: 43157

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHHKYLIVGGGMTADSAVHGIRKIDPDGAIAVIGEELHRPYNRPPLSKALWKDSPYDSI
CCCCEEEEEECCCCHHHHHHHHHEECCCCCEEEEEHHHCCCCCCCCCHHHHCCCCCHHHH
WRSEHGLNVAMHLGKKIVALDPANKTATDNAGNIYTYEKLLLATGGEVRRFPHFDSGIIY
HHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCEEE
YRTADDYLKLRELSSQGSDFVVIGGGFIGSEIAAALAMNDKRVTMIFPENGISSRIYPRP
EECCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCHH
LVEFLNSYYREKGVIVLAPETVTSIRTDGTKKIVTTGSGTEISADGVVAGLGILPNTELA
HHHHHHHHHHHCCEEEECCCHHHHHCCCCCEEEEECCCCCEEECCCEEEECCCCCCCHHH
VQAGLAIDNGIVVDEFLRTSNPDIYAAGDVANFYSPLLDKRMRVEHEDNANMMGEAAGRN
EEECCEECCCEEHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
MAGSLEPYHHQPFFYSDLFDLGYEAVGELDSSLDIVEDWVEPFRKGVIYYLRDELVRGVL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEHEEHHHHHCEEE
LWNTWGQVDAATALIAEKKPCTNETLLGRIKD
EECCCCCHHHHHHHHHCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MKHHKYLIVGGGMTADSAVHGIRKIDPDGAIAVIGEELHRPYNRPPLSKALWKDSPYDSI
CCCCEEEEEECCCCHHHHHHHHHEECCCCCEEEEEHHHCCCCCCCCCHHHHCCCCCHHHH
WRSEHGLNVAMHLGKKIVALDPANKTATDNAGNIYTYEKLLLATGGEVRRFPHFDSGIIY
HHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCEEE
YRTADDYLKLRELSSQGSDFVVIGGGFIGSEIAAALAMNDKRVTMIFPENGISSRIYPRP
EECCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHEECCCEEEEEECCCCCCCCCCCHH
LVEFLNSYYREKGVIVLAPETVTSIRTDGTKKIVTTGSGTEISADGVVAGLGILPNTELA
HHHHHHHHHHHCCEEEECCCHHHHHCCCCCEEEEECCCCCEEECCCEEEECCCCCCCHHH
VQAGLAIDNGIVVDEFLRTSNPDIYAAGDVANFYSPLLDKRMRVEHEDNANMMGEAAGRN
EEECCEECCCEEHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
MAGSLEPYHHQPFFYSDLFDLGYEAVGELDSSLDIVEDWVEPFRKGVIYYLRDELVRGVL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEHEEHHHHHCEEE
LWNTWGQVDAATALIAEKKPCTNETLLGRIKD
EECCCCCHHHHHHHHHCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]