Click here to switch to the map view.

The map label for this gene is rffA [H]

Identifier: 82701422

GI number: 82701422

Start: 308676

End: 309800

Strand: Direct

Name: rffA [H]

Synonym: Nmul_A0288

Alternate gene names: 82701422

Gene position: 308676-309800 (Clockwise)

Preceding gene: 82701421

Following gene: 82701423

Centisome position: 9.69

GC content: 54.67

Gene sequence:

>1125_bases
ATGCAAATACCGTTCAACAAACCCTATATGACCGGGAAGGAGCTATGGTATGTCAGCCAGGCTCATGCGAACGGACATCT
GGCCGGCGACGGGGTTTTCACGAAGAAATGCAATGCGTGGCTGGAACGCCGGATCGGGTGTAAAAAAGCGTTGCTGACGC
ATTCCTGCACGGCTGCGCTGGAGATGGCGGCCATTCTTGCGCAGATCGGACCCGGCGATGAGGTCATCATGCCTTCCTAT
ACTTTCGTCTCCACCGCCAATGCGTTCGCATTGAGAGGGGGCGTGCCTGTATTCGTGGACATCCGGCCTGATACATTCAA
TATCGACGAAACCCTGATCGAGGCTGCCATCACCCCGAAAACCAAAGCGATCGTTGCTGTTCACTACGCGGGAGTGGGAT
GCGAGATGGATGCCATCATGTCCATCGCCGAACGGTATGGTTTGTTCGTGATCGAGGATGCAGCCCAGGGTCTTATGTCA
ACCTACAAGGGCAGACCGCTGGGCAGTATCGGCCATCTCGCAGCGCTGAGCTTCCACGAGACCAAGAACATCATTTCCGG
GGAAGGGGGAGCTCTGCTGGTGAACGACGCACGCTTTGCGGAGCAAGCGGAAATAATCAGGGAAAAAGGTACCAATCGCA
GTCAGTTTTTTCGTGGGCAGGTGGATAAGTATACCTGGGTGGATTTCGGCTCATCCTATCTTCCCAGTGAACTCATTGCT
GCCTTTCTCTGGGCTCAGATAGAGGAGGCGGATGCAATCACGCAACGCCGTCTCGATATCTGGGACGTCTACCACGCTAA
TTTCGAACTTTTGGAAAAACAGGGGAAGATCCATCGTCCGACCATTCCTTCTGATTGCATGCACAACGCCCATATGTACT
ATCTCCTGTTGCCCGACCTGGACAGGCGCACCGCTTTCATCAACGCTCTCCGGCAGAAGGGAATAGGAACAGTTTTTCAT
TACGTTCCTCTTGACAGCTCTCCCATGGGGGAAAAGTGGGGCCGCAAGTCAGGCGCATTAACGCGGACACGAGAGTTGAG
CGACCGGCTGGTCAGGCTGCCGCTATGGCTGGGCCTGGAGGAATACCAGAACGAAGTCATTCAGCATGTCGCGGCCGAGT
TATGA

Upstream 100 bases:

>100_bases
CTTGATCTGGAACGTCTGATGATTCGCGGGCGAGTCTCATTTCCGATGGGCGGGTCGCGATTGCTGATTGCCCGAAAACC
AGCAGGGGCGTAATCACCAT

Downstream 100 bases:

>100_bases
AAAAGTCACGTTCCAGTTTCCATATTCTTTGCATAAGTCATCTGGCGCGGCTTCCTATATGTTCCTGACTGGAGTTTCGC
GTAAATCTTCCGTTAAGCCA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MQIPFNKPYMTGKELWYVSQAHANGHLAGDGVFTKKCNAWLERRIGCKKALLTHSCTAALEMAAILAQIGPGDEVIMPSY
TFVSTANAFALRGGVPVFVDIRPDTFNIDETLIEAAITPKTKAIVAVHYAGVGCEMDAIMSIAERYGLFVIEDAAQGLMS
TYKGRPLGSIGHLAALSFHETKNIISGEGGALLVNDARFAEQAEIIREKGTNRSQFFRGQVDKYTWVDFGSSYLPSELIA
AFLWAQIEEADAITQRRLDIWDVYHANFELLEKQGKIHRPTIPSDCMHNAHMYYLLLPDLDRRTAFINALRQKGIGTVFH
YVPLDSSPMGEKWGRKSGALTRTRELSDRLVRLPLWLGLEEYQNEVIQHVAAEL

Sequences:

>Translated_374_residues
MQIPFNKPYMTGKELWYVSQAHANGHLAGDGVFTKKCNAWLERRIGCKKALLTHSCTAALEMAAILAQIGPGDEVIMPSY
TFVSTANAFALRGGVPVFVDIRPDTFNIDETLIEAAITPKTKAIVAVHYAGVGCEMDAIMSIAERYGLFVIEDAAQGLMS
TYKGRPLGSIGHLAALSFHETKNIISGEGGALLVNDARFAEQAEIIREKGTNRSQFFRGQVDKYTWVDFGSSYLPSELIA
AFLWAQIEEADAITQRRLDIWDVYHANFELLEKQGKIHRPTIPSDCMHNAHMYYLLLPDLDRRTAFINALRQKGIGTVFH
YVPLDSSPMGEKWGRKSGALTRTRELSDRLVRLPLWLGLEEYQNEVIQHVAAEL
>Mature_374_residues
MQIPFNKPYMTGKELWYVSQAHANGHLAGDGVFTKKCNAWLERRIGCKKALLTHSCTAALEMAAILAQIGPGDEVIMPSY
TFVSTANAFALRGGVPVFVDIRPDTFNIDETLIEAAITPKTKAIVAVHYAGVGCEMDAIMSIAERYGLFVIEDAAQGLMS
TYKGRPLGSIGHLAALSFHETKNIISGEGGALLVNDARFAEQAEIIREKGTNRSQFFRGQVDKYTWVDFGSSYLPSELIA
AFLWAQIEEADAITQRRLDIWDVYHANFELLEKQGKIHRPTIPSDCMHNAHMYYLLLPDLDRRTAFINALRQKGIGTVFH
YVPLDSSPMGEKWGRKSGALTRTRELSDRLVRLPLWLGLEEYQNEVIQHVAAEL

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=366, Percent_Identity=60.9289617486339, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI145693159, Length=370, Percent_Identity=33.2432432432432, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41720; Mature: 41720

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPFNKPYMTGKELWYVSQAHANGHLAGDGVFTKKCNAWLERRIGCKKALLTHSCTAAL
CCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
EMAAILAQIGPGDEVIMPSYTFVSTANAFALRGGVPVFVDIRPDTFNIDETLIEAAITPK
HHHHHHHHCCCCCCEEECCCHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCC
TKAIVAVHYAGVGCEMDAIMSIAERYGLFVIEDAAQGLMSTYKGRPLGSIGHLAALSFHE
CCEEEEEEECCCCCCHHHHHHHHHHHCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TKNIISGEGGALLVNDARFAEQAEIIREKGTNRSQFFRGQVDKYTWVDFGSSYLPSELIA
HHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHH
AFLWAQIEEADAITQRRLDIWDVYHANFELLEKQGKIHRPTIPSDCMHNAHMYYLLLPDL
HHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCC
DRRTAFINALRQKGIGTVFHYVPLDSSPMGEKWGRKSGALTRTRELSDRLVRLPLWLGLE
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
EYQNEVIQHVAAEL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MQIPFNKPYMTGKELWYVSQAHANGHLAGDGVFTKKCNAWLERRIGCKKALLTHSCTAAL
CCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHH
EMAAILAQIGPGDEVIMPSYTFVSTANAFALRGGVPVFVDIRPDTFNIDETLIEAAITPK
HHHHHHHHCCCCCCEEECCCHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHCCCC
TKAIVAVHYAGVGCEMDAIMSIAERYGLFVIEDAAQGLMSTYKGRPLGSIGHLAALSFHE
CCEEEEEEECCCCCCHHHHHHHHHHHCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
TKNIISGEGGALLVNDARFAEQAEIIREKGTNRSQFFRGQVDKYTWVDFGSSYLPSELIA
HHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHH
AFLWAQIEEADAITQRRLDIWDVYHANFELLEKQGKIHRPTIPSDCMHNAHMYYLLLPDL
HHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCC
DRRTAFINALRQKGIGTVFHYVPLDSSPMGEKWGRKSGALTRTRELSDRLVRLPLWLGLE
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
EYQNEVIQHVAAEL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]