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The map label for this gene is rluF [H]

Identifier: 82701397

GI number: 82701397

Start: 285301

End: 286032

Strand: Direct

Name: rluF [H]

Synonym: Nmul_A0263

Alternate gene names: 82701397

Gene position: 285301-286032 (Clockwise)

Preceding gene: 82701396

Following gene: 82701398

Centisome position: 8.96

GC content: 51.23

Gene sequence:

>732_bases
ATGGAAAAAATCCGGTTATCCAAGCTTATGTCCCAGAAGGGGTTGTGTTCGCGCCGGGAAGCGGATGATTATATCGAACG
CGGCTGGGTATTTGTGGATGGTGAGCGCATCAGTCAATTAGGTATCAAGGTTTTTCCTACGCAGGAAATAAAGCTTGCGC
GCATGGCGCAAGCGCAGCAGTGTGATCAAATTACCGTACTGTTGCATAAGCCCGTCGGGTATGTTTCAGGACAACCCGAG
CGAGGATACAAGCCCGCGATCAGCCTCATCAATAGAGAATCGGAGTTTCAGGATAGCAAGGCATCATCCAGTTTTAGCCC
ACTGAATTTGAAAGGCATGGCGCCGGCGGGCCGGTTGGATATCGATTCAAGCGGATTGCTCGTGTTTACCCAGGACGGTC
GCATCGCAAGGAAATTGATCGGTGCCGAATCCGAGATTGAAAAGGAGTATCTTGTACGTGCGGAAGGAGAGGTGTCCCAG
CGTACCCTGAATCTGTTAAGTCACGGCTTGAGTCTGGATGGGCAGGCACTGAAACGTGCCGTGGTGGTCCAGCAAAATAA
GGACCAGTTGAGGTTCATTTTAAAGGAAGGTAGGAAGCGGCAGATTCGTAGAATGTGCGAACTAGTTGGTTTGAAGGTCA
CAGGACTGAAACGCGTTCGCATCGGCAAGGTAAGGCTGGGCGAGCTTCCCTTGGGAAAGTGGCGTTATTTGCGGCCAGGC
GAAGAGTTTTAA

Upstream 100 bases:

>100_bases
TTTTAGCTGAAACGGTGCGGACGTTCAAACTATAATGTGCAATTCTTGAACGATGTGTCGTTATGTTTCGTGAATCTTTT
TCGTTCAGCCGCAGGAAGCA

Downstream 100 bases:

>100_bases
CCCCGTATTCTGCGTTTTTTCCATTTTGCTATCATCAGGATTTATCGGGAGGACCAGGCGGTAAGGGTGATTAAACAACA
TAACGATAACTTAATAAGTT

Product: pseudouridine synthase Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MEKIRLSKLMSQKGLCSRREADDYIERGWVFVDGERISQLGIKVFPTQEIKLARMAQAQQCDQITVLLHKPVGYVSGQPE
RGYKPAISLINRESEFQDSKASSSFSPLNLKGMAPAGRLDIDSSGLLVFTQDGRIARKLIGAESEIEKEYLVRAEGEVSQ
RTLNLLSHGLSLDGQALKRAVVVQQNKDQLRFILKEGRKRQIRRMCELVGLKVTGLKRVRIGKVRLGELPLGKWRYLRPG
EEF

Sequences:

>Translated_243_residues
MEKIRLSKLMSQKGLCSRREADDYIERGWVFVDGERISQLGIKVFPTQEIKLARMAQAQQCDQITVLLHKPVGYVSGQPE
RGYKPAISLINRESEFQDSKASSSFSPLNLKGMAPAGRLDIDSSGLLVFTQDGRIARKLIGAESEIEKEYLVRAEGEVSQ
RTLNLLSHGLSLDGQALKRAVVVQQNKDQLRFILKEGRKRQIRRMCELVGLKVTGLKRVRIGKVRLGELPLGKWRYLRPG
EEF
>Mature_243_residues
MEKIRLSKLMSQKGLCSRREADDYIERGWVFVDGERISQLGIKVFPTQEIKLARMAQAQQCDQITVLLHKPVGYVSGQPE
RGYKPAISLINRESEFQDSKASSSFSPLNLKGMAPAGRLDIDSSGLLVFTQDGRIARKLIGAESEIEKEYLVRAEGEVSQ
RTLNLLSHGLSLDGQALKRAVVVQQNKDQLRFILKEGRKRQIRRMCELVGLKVTGLKRVRIGKVRLGELPLGKWRYLRPG
EEF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=35, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=33.0578512396694, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=30.8016877637131, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=34.4827586206897, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27478; Mature: 27478

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIRLSKLMSQKGLCSRREADDYIERGWVFVDGERISQLGIKVFPTQEIKLARMAQAQQ
CCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCHHHHHCCEEEECCCHHHHHHHHHHHH
CDQITVLLHKPVGYVSGQPERGYKPAISLINRESEFQDSKASSSFSPLNLKGMAPAGRLD
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEE
IDSSGLLVFTQDGRIARKLIGAESEIEKEYLVRAEGEVSQRTLNLLSHGLSLDGQALKRA
ECCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
VVVQQNKDQLRFILKEGRKRQIRRMCELVGLKVTGLKRVRIGKVRLGELPLGKWRYLRPG
HHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCEEECCCC
EEF
CCC
>Mature Secondary Structure
MEKIRLSKLMSQKGLCSRREADDYIERGWVFVDGERISQLGIKVFPTQEIKLARMAQAQQ
CCCHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCHHHHHCCEEEECCCHHHHHHHHHHHH
CDQITVLLHKPVGYVSGQPERGYKPAISLINRESEFQDSKASSSFSPLNLKGMAPAGRLD
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEE
IDSSGLLVFTQDGRIARKLIGAESEIEKEYLVRAEGEVSQRTLNLLSHGLSLDGQALKRA
ECCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
VVVQQNKDQLRFILKEGRKRQIRRMCELVGLKVTGLKRVRIGKVRLGELPLGKWRYLRPG
HHHCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCEEECCCC
EEF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]