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The map label for this gene is rfbB [H]

Identifier: 82701398

GI number: 82701398

Start: 286146

End: 287207

Strand: Direct

Name: rfbB [H]

Synonym: Nmul_A0264

Alternate gene names: 82701398

Gene position: 286146-287207 (Clockwise)

Preceding gene: 82701397

Following gene: 82701399

Centisome position: 8.99

GC content: 51.69

Gene sequence:

>1062_bases
ATGATTCTGGTAACAGGAGGCGCCGGATTTATCGGGGCAAATTTCGTATTGGATTGGTTGGAGCGATCTGACGAGGCGAT
CATCAACCTCGATGTGCTCACCTATGCTGGCAACCTGGAGAACCTGAAAACGCTGGAGGGTGACAAGCGGCATGTTTTTG
TACAAGGCGATATTGGTAATTCCGACCTCCTGATTCATCTGCTAAAGGACTATCGGCCGCGGGCCATCCTTAATTTTGCC
GCGGAGAGTCATGTCGATCGTTCCATACATGGTCCGGAGGACTTCATACAGACCAACATCGTGGGTACCTTCCGTCTTCT
GGAAGCATCTCGCGATTACTGGAGCAGATTGAAGGAAAATGAAAAACAGCAGTTTCGTTTTCTGCACGTCTCAACCGATG
AGGTTTATGGCTCGCTGGCAAAAGACGAAATGCCTTTTACCGAGACCCATCGTTACGAACCCAACAGCCCTTACTCGGCA
AGCAAGGCAGCAAGCGATCATTTGGTGCGCGCCTATCACCACACCTACGGGTTGCCTGTTCTCACCACAAACTGCTCCAA
CAATTACGGGCCATATCAATTTCCCGAAAAGCTCATCCCGCTTATGATCGTCAATGCACTGGCGGGAAAGTCATTGCCGG
TCTACGGCGATGGCCAGCAAATCCGTGACTGGCTCTACGTAAAAGATCATTGCAGCGCCATTCGCCGTGTGCTGGAGGAT
GGCATTCCGGGACAGATTTATAATATCGGCGGCTGGAATGAAAAACCCAACATCGAAATTGTGGAAACAATCTGTAGCCT
GCTCGACACGTTCCGTCCCCGGGAGGGCCACTCCTATCGCAATCTGATTAAATATGTGGCGGATCGTCCAGGCCATGACC
GGCGCTATGCGATCGATGCGAGCAAAATCGAGCGTGAGCTGGGTTGGAGACCTGCTGAAACTTTTGAAACGGGAATCAGC
AAAACCGTGCAATGGTATCTCGACAACGCGGCTTGGGTCACCCATGTTCAATCCGGCGCTTACCGGCACTGGGTGGAGAA
GAACTACAAAGAGCGCGCTTGA

Upstream 100 bases:

>100_bases
GTTTTTTCCATTTTGCTATCATCAGGATTTATCGGGAGGACCAGGCGGTAAGGGTGATTAAACAACATAACGATAACTTA
ATAAGTTCAAGGAAGAGCAA

Downstream 100 bases:

>100_bases
TGAAAATTCTGCTGTTCGGAAAAGACGGCCAGGTTGGGTGGGAACTGCAGCGCAGCCTTGCCCCCCTGGGGGAGATAGTG
GCTCTGGATTTTGGCAGCCA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLERSDEAIINLDVLTYAGNLENLKTLEGDKRHVFVQGDIGNSDLLIHLLKDYRPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEASRDYWSRLKENEKQQFRFLHVSTDEVYGSLAKDEMPFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGKSLPVYGDGQQIRDWLYVKDHCSAIRRVLED
GIPGQIYNIGGWNEKPNIEIVETICSLLDTFRPREGHSYRNLIKYVADRPGHDRRYAIDASKIERELGWRPAETFETGIS
KTVQWYLDNAAWVTHVQSGAYRHWVEKNYKERA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLERSDEAIINLDVLTYAGNLENLKTLEGDKRHVFVQGDIGNSDLLIHLLKDYRPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEASRDYWSRLKENEKQQFRFLHVSTDEVYGSLAKDEMPFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGKSLPVYGDGQQIRDWLYVKDHCSAIRRVLED
GIPGQIYNIGGWNEKPNIEIVETICSLLDTFRPREGHSYRNLIKYVADRPGHDRRYAIDASKIERELGWRPAETFETGIS
KTVQWYLDNAAWVTHVQSGAYRHWVEKNYKERA
>Mature_353_residues
MILVTGGAGFIGANFVLDWLERSDEAIINLDVLTYAGNLENLKTLEGDKRHVFVQGDIGNSDLLIHLLKDYRPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEASRDYWSRLKENEKQQFRFLHVSTDEVYGSLAKDEMPFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIVNALAGKSLPVYGDGQQIRDWLYVKDHCSAIRRVLED
GIPGQIYNIGGWNEKPNIEIVETICSLLDTFRPREGHSYRNLIKYVADRPGHDRRYAIDASKIERELGWRPAETFETGIS
KTVQWYLDNAAWVTHVQSGAYRHWVEKNYKERA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=37.8787878787879, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=23.030303030303, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4504031, Length=347, Percent_Identity=24.4956772334294, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56237023, Length=358, Percent_Identity=27.0949720670391, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=358, Percent_Identity=27.0949720670391, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=358, Percent_Identity=27.0949720670391, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=59.3314763231198, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=59.8837209302326, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=27.2206303724928, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788366, Length=312, Percent_Identity=26.2820512820513, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788365, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=37.6899696048632, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=32.2188449848024, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17507723, Length=193, Percent_Identity=31.0880829015544, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.9393939393939, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=23.7569060773481, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356223, Length=348, Percent_Identity=25.2873563218391, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24158427, Length=286, Percent_Identity=26.5734265734266, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=25.8241758241758, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40474; Mature: 40474

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLERSDEAIINLDVLTYAGNLENLKTLEGDKRHVFVQGDIGN
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHCCCCCEEEEEECCCCC
SDLLIHLLKDYRPRAILNFAAESHVDRSIHGPEDFIQTNIVGTFRLLEASRDYWSRLKEN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EKQQFRFLHVSTDEVYGSLAKDEMPFTETHRYEPNSPYSASKAASDHLVRAYHHTYGLPV
CHHHEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIVNALAGKSLPVYGDGQQIRDWLYVKDHCSAIRRVLED
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GIPGQIYNIGGWNEKPNIEIVETICSLLDTFRPREGHSYRNLIKYVADRPGHDRRYAIDA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECH
SKIERELGWRPAETFETGISKTVQWYLDNAAWVTHVQSGAYRHWVEKNYKERA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLERSDEAIINLDVLTYAGNLENLKTLEGDKRHVFVQGDIGN
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHCCCCCEEEEEECCCCC
SDLLIHLLKDYRPRAILNFAAESHVDRSIHGPEDFIQTNIVGTFRLLEASRDYWSRLKEN
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EKQQFRFLHVSTDEVYGSLAKDEMPFTETHRYEPNSPYSASKAASDHLVRAYHHTYGLPV
CHHHEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIVNALAGKSLPVYGDGQQIRDWLYVKDHCSAIRRVLED
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GIPGQIYNIGGWNEKPNIEIVETICSLLDTFRPREGHSYRNLIKYVADRPGHDRRYAIDA
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECH
SKIERELGWRPAETFETGISKTVQWYLDNAAWVTHVQSGAYRHWVEKNYKERA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]