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The map label for this gene is lolA

Identifier: 82701312

GI number: 82701312

Start: 188945

End: 189568

Strand: Direct

Name: lolA

Synonym: Nmul_A0178

Alternate gene names: 82701312

Gene position: 188945-189568 (Clockwise)

Preceding gene: 82701310

Following gene: 82701313

Centisome position: 5.93

GC content: 49.84

Gene sequence:

>624_bases
ATGAAGCTATCCGAAAAGTTCTGTGTTTTCCTTTTTTTCCTTTTATTTACTTCCACAACCCATGCAACCGGAATCGGCAG
TCTCAAGAGCTTCATGGAGAATGCGCGGACTGTCCGCGCAGATTTTGCGCAAACCGTTCTCGACAAAAGTCGCCACATAG
TGCAGACAGCAAGCGGTGCCATGCAATTCGAGCGTCCCGGAAAATTCCGCTGGATATACGAGAAGCCTTACGAACAATTG
ATCGTTGGAGACGGCGAGCGCATCTGGTTTTACGATCATGATCTTGCTCAGGTAACCGTGCGAAAGCTGGATGCCGCCAT
TGGCAGCAGTCCTGCCGCGCTGCTTGCCGGAAACAATGATATCGAAAAGAATTTCCATCTTTTGGAAATCGGCCTGCAGG
GTAACATCGAATGGGTGGAAGCCACACCCAAAACCCGGGAAAGTACATTTGAGCGGGTGCTTCTCGGATTTACCCTGGAG
GGGATACTTAGAGTCATGGAATTGCATGATAATTTCGGCCAGGTTACCGTATTCGAATTTTCGGGTGTGGAACAAAACCG
CAAGCTTCCTCCGGAATTGTTCAAATTCAGTCCCCCGGCGGGTGTGGACGTGATCAGTGAGTGA

Upstream 100 bases:

>100_bases
GAACTTCGAGAGATCGCCTTAAATTGCCCGACGAGGGAGCGGCTTATTTATAATTACCGTGATCCTGTTCTTTGCCTGGT
TCCAATCCCTAGTCAAAATC

Downstream 100 bases:

>100_bases
TCTGTTCGCGCAGGCCCAACCTGCTGCACCCCTGGCGGAACAGTTGCGTCCGCATGAATTGAAGGATGTGGTGGGGCAGC
CTCATTTGTTGGGTCCCGGC

Product: outer membrane lipoprotein carrier protein LolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKLSEKFCVFLFFLLFTSTTHATGIGSLKSFMENARTVRADFAQTVLDKSRHIVQTASGAMQFERPGKFRWIYEKPYEQL
IVGDGERIWFYDHDLAQVTVRKLDAAIGSSPAALLAGNNDIEKNFHLLEIGLQGNIEWVEATPKTRESTFERVLLGFTLE
GILRVMELHDNFGQVTVFEFSGVEQNRKLPPELFKFSPPAGVDVISE

Sequences:

>Translated_207_residues
MKLSEKFCVFLFFLLFTSTTHATGIGSLKSFMENARTVRADFAQTVLDKSRHIVQTASGAMQFERPGKFRWIYEKPYEQL
IVGDGERIWFYDHDLAQVTVRKLDAAIGSSPAALLAGNNDIEKNFHLLEIGLQGNIEWVEATPKTRESTFERVLLGFTLE
GILRVMELHDNFGQVTVFEFSGVEQNRKLPPELFKFSPPAGVDVISE
>Mature_207_residues
MKLSEKFCVFLFFLLFTSTTHATGIGSLKSFMENARTVRADFAQTVLDKSRHIVQTASGAMQFERPGKFRWIYEKPYEQL
IVGDGERIWFYDHDLAQVTVRKLDAAIGSSPAALLAGNNDIEKNFHLLEIGLQGNIEWVEATPKTRESTFERVLLGFTLE
GILRVMELHDNFGQVTVFEFSGVEQNRKLPPELFKFSPPAGVDVISE

Specific function: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the l

COG id: COG2834

COG function: function code M; Outer membrane lipoprotein-sorting protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolA family

Homologues:

Organism=Escherichia coli, GI145693108, Length=197, Percent_Identity=26.9035532994924, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): LOLA_NITMU (Q2YCN5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410878.1
- ProteinModelPortal:   Q2YCN5
- SMR:   Q2YCN5
- STRING:   Q2YCN5
- GeneID:   3785086
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0178
- eggNOG:   COG2834
- HOGENOM:   HBG688940
- OMA:   VDIDDQR
- PhylomeDB:   Q2YCN5
- ProtClustDB:   CLSK585616
- BioCyc:   NMUL323848:NMUL_A0178-MONOMER
- HAMAP:   MF_00240
- InterPro:   IPR004564
- InterPro:   IPR018323
- TIGRFAMs:   TIGR00547

Pfam domain/function: PF03548 LolA

EC number: NA

Molecular weight: Translated: 23436; Mature: 23436

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSEKFCVFLFFLLFTSTTHATGIGSLKSFMENARTVRADFAQTVLDKSRHIVQTASGA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQFERPGKFRWIYEKPYEQLIVGDGERIWFYDHDLAQVTVRKLDAAIGSSPAALLAGNND
EEECCCCCEEEEECCCCHHEEECCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCC
IEKNFHLLEIGLQGNIEWVEATPKTRESTFERVLLGFTLEGILRVMELHDNFGQVTVFEF
CCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SGVEQNRKLPPELFKFSPPAGVDVISE
CCCCCCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLSEKFCVFLFFLLFTSTTHATGIGSLKSFMENARTVRADFAQTVLDKSRHIVQTASGA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQFERPGKFRWIYEKPYEQLIVGDGERIWFYDHDLAQVTVRKLDAAIGSSPAALLAGNND
EEECCCCCEEEEECCCCHHEEECCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCC
IEKNFHLLEIGLQGNIEWVEATPKTRESTFERVLLGFTLEGILRVMELHDNFGQVTVFEF
CCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SGVEQNRKLPPELFKFSPPAGVDVISE
CCCCCCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA