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The map label for this gene is glcE [H]

Identifier: 82701308

GI number: 82701308

Start: 182374

End: 183468

Strand: Direct

Name: glcE [H]

Synonym: Nmul_A0174

Alternate gene names: 82701308

Gene position: 182374-183468 (Clockwise)

Preceding gene: 82701307

Following gene: 82701309

Centisome position: 5.73

GC content: 58.08

Gene sequence:

>1095_bases
ATGCAGGAAATCGTTGATCAGCTTGCGGCGACCATTCGCGCCGCTGCGGCGAGTAACGTGCCGCTACGTATCCGCAGCGG
CGGCACCAAGGATTTTTATGGCCAGCAGGACAAAGGACGAGACGTCGCGCCCGAAGGCATTCTCGATCCCACGGCTTTTG
CGGGTATAGTGGATTACGAACCCACCGAACTGGTCGTGACCGCGCGGACGGGCACCCCCCTGGCGCAACTGGAAACCGAA
TTGAATGGTCGGGGTCAGATGCTTGCGTTCGAGCCACCGCATTTCGGGGCGGGCGCCACCCTTGGCGGCTGTATCGCGGC
AGGCTTATCCGGGCCACGCCGCGCCTCGGCAGGCGCCGTACGAGACTTCGTGCTGGGGGTGCGCATGCTGGATGGCAGAG
GGGATGACTTGAGTTTTGGCGGCCGGGTAATGAAGAACGTGGCTGGTTATGACATCCCACGTTTAATGGCGGGTTCCCTG
GGCACATTGGGATTATTGCTGGAAGTTTCCCTGAAAGTGCTTCCCCTGCCCGCAGAGGAACGCACGCTGCGTTTATCGAT
GAATGAAGCCGTAGCCATTGAGACCATGAATCGCTGGGCAGGCAAGCCCCTGCCGGTTTCAGCAACCTGTTTCTGCGAGG
GTGAGCTCACTGTAAGGTTATCCGGCGCAACTTCCGCAGTGCGTGCGGCGCGCGCGGTATTGGGAGGAGAGGAAATTGTC
GAAAATTCGTCTTTCTGGAAGTCGATACGGGAGCAGGTACATCCTTTTTTTCGATCAGGAAAGCAACTTTGGCGCTTGTC
CATTAAATCGACCACCGCTCCTCTGTCCTTGCCTGGAAAACAGTTGATCGAGTGGGGGGGTGCGGTGCGATGGCTCTCTG
TCGATGCAGAGATGGATAACGACGCTGTGGAGACACTTGTCCGCAAGACCGCAAAGGAGGCCGGGGGACATGCCACTCTG
TTCCGTAGCCGGACTCCCGCTGCTGCTGTGTTTCATCCGCTTCCGCCGGCCATGATGAATATTCACCGCCAGCTCAAGGA
GAAATTTGACCCGATGCGTATTTTCAATCGGGGCCGTCTATATCCCGAACTATAA

Upstream 100 bases:

>100_bases
ATGCACGTGCATCGGGGCAAAGAGAAATTCCCCGAATTGCCGAGATTTTGAAGTCATGTCCATTTCATATACTCTTCACG
GTCTTACAGGCTGACGCAGT

Downstream 100 bases:

>100_bases
ATGCAAACCAATCTCGCTGATTTCATCAAGGATACTCCCGAGGGAAAGGAAGCTGAGGCAATATTGCGAACCTGCGTTCA
TTGCGGTTTCTGCCTGGCTA

Product: glycolate oxidase FAD binding subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQEIVDQLAATIRAAAASNVPLRIRSGGTKDFYGQQDKGRDVAPEGILDPTAFAGIVDYEPTELVVTARTGTPLAQLETE
LNGRGQMLAFEPPHFGAGATLGGCIAAGLSGPRRASAGAVRDFVLGVRMLDGRGDDLSFGGRVMKNVAGYDIPRLMAGSL
GTLGLLLEVSLKVLPLPAEERTLRLSMNEAVAIETMNRWAGKPLPVSATCFCEGELTVRLSGATSAVRAARAVLGGEEIV
ENSSFWKSIREQVHPFFRSGKQLWRLSIKSTTAPLSLPGKQLIEWGGAVRWLSVDAEMDNDAVETLVRKTAKEAGGHATL
FRSRTPAAAVFHPLPPAMMNIHRQLKEKFDPMRIFNRGRLYPEL

Sequences:

>Translated_364_residues
MQEIVDQLAATIRAAAASNVPLRIRSGGTKDFYGQQDKGRDVAPEGILDPTAFAGIVDYEPTELVVTARTGTPLAQLETE
LNGRGQMLAFEPPHFGAGATLGGCIAAGLSGPRRASAGAVRDFVLGVRMLDGRGDDLSFGGRVMKNVAGYDIPRLMAGSL
GTLGLLLEVSLKVLPLPAEERTLRLSMNEAVAIETMNRWAGKPLPVSATCFCEGELTVRLSGATSAVRAARAVLGGEEIV
ENSSFWKSIREQVHPFFRSGKQLWRLSIKSTTAPLSLPGKQLIEWGGAVRWLSVDAEMDNDAVETLVRKTAKEAGGHATL
FRSRTPAAAVFHPLPPAMMNIHRQLKEKFDPMRIFNRGRLYPEL
>Mature_364_residues
MQEIVDQLAATIRAAAASNVPLRIRSGGTKDFYGQQDKGRDVAPEGILDPTAFAGIVDYEPTELVVTARTGTPLAQLETE
LNGRGQMLAFEPPHFGAGATLGGCIAAGLSGPRRASAGAVRDFVLGVRMLDGRGDDLSFGGRVMKNVAGYDIPRLMAGSL
GTLGLLLEVSLKVLPLPAEERTLRLSMNEAVAIETMNRWAGKPLPVSATCFCEGELTVRLSGATSAVRAARAVLGGEEIV
ENSSFWKSIREQVHPFFRSGKQLWRLSIKSTTAPLSLPGKQLIEWGGAVRWLSVDAEMDNDAVETLVRKTAKEAGGHATL
FRSRTPAAAVFHPLPPAMMNIHRQLKEKFDPMRIFNRGRLYPEL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI48994914, Length=357, Percent_Identity=46.218487394958, Blast_Score=311, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6320027, Length=184, Percent_Identity=26.6304347826087, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 39273; Mature: 39273

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIVDQLAATIRAAAASNVPLRIRSGGTKDFYGQQDKGRDVAPEGILDPTAFAGIVDYE
CHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEECCC
PTELVVTARTGTPLAQLETELNGRGQMLAFEPPHFGAGATLGGCIAAGLSGPRRASAGAV
CCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHH
RDFVLGVRMLDGRGDDLSFGGRVMKNVAGYDIPRLMAGSLGTLGLLLEVSLKVLPLPAEE
HHHHHHHHEECCCCCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHEEECCCCC
RTLRLSMNEAVAIETMNRWAGKPLPVSATCFCEGELTVRLSGATSAVRAARAVLGGEEIV
CEEEEECCCEEEEEEHHHHCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHCHHHHH
ENSSFWKSIREQVHPFFRSGKQLWRLSIKSTTAPLSLPGKQLIEWGGAVRWLSVDAEMDN
CCHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCH
DAVETLVRKTAKEAGGHATLFRSRTPAAAVFHPLPPAMMNIHRQLKEKFDPMRIFNRGRL
HHHHHHHHHHHHHCCCCEEEEECCCCCHHEECCCCHHHHHHHHHHHHHCCHHHHHHCCCC
YPEL
CCCC
>Mature Secondary Structure
MQEIVDQLAATIRAAAASNVPLRIRSGGTKDFYGQQDKGRDVAPEGILDPTAFAGIVDYE
CHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEECCC
PTELVVTARTGTPLAQLETELNGRGQMLAFEPPHFGAGATLGGCIAAGLSGPRRASAGAV
CCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHH
RDFVLGVRMLDGRGDDLSFGGRVMKNVAGYDIPRLMAGSLGTLGLLLEVSLKVLPLPAEE
HHHHHHHHEECCCCCCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHEEECCCCC
RTLRLSMNEAVAIETMNRWAGKPLPVSATCFCEGELTVRLSGATSAVRAARAVLGGEEIV
CEEEEECCCEEEEEEHHHHCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHCHHHHH
ENSSFWKSIREQVHPFFRSGKQLWRLSIKSTTAPLSLPGKQLIEWGGAVRWLSVDAEMDN
CCHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCH
DAVETLVRKTAKEAGGHATLFRSRTPAAAVFHPLPPAMMNIHRQLKEKFDPMRIFNRGRL
HHHHHHHHHHHHHCCCCEEEEECCCCCHHEECCCCHHHHHHHHHHHHHCCHHHHHHCCCC
YPEL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]