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The map label for this gene is glcF [H]

Identifier: 82701309

GI number: 82701309

Start: 183469

End: 184818

Strand: Direct

Name: glcF [H]

Synonym: Nmul_A0175

Alternate gene names: 82701309

Gene position: 183469-184818 (Clockwise)

Preceding gene: 82701308

Following gene: 82701310

Centisome position: 5.76

GC content: 58.07

Gene sequence:

>1350_bases
ATGCAAACCAATCTCGCTGATTTCATCAAGGATACTCCCGAGGGAAAGGAAGCTGAGGCAATATTGCGAACCTGCGTTCA
TTGCGGTTTCTGCCTGGCTACCTGTCCGACTTATCAATTACTGGGAGACGAGCTCGATAGCCCGCGCGGGCGCATCTATC
TGATGAAGCAGATGCTGGAAGGCGAGCCGGTGACGGAAAAGACGCAATTGCATCTCGACCGCTGCCTCACCTGCCGCGCT
TGCGAGACAGCCTGCCCGTCCGGCGTGCGTTACGGTCATCTGGTGGATATCGGCCGGGGCCTTGTCGAAAAGAAGGTGGG
GCGCAGCCTTGCTGCCGGAAGCATGCGATATGTGTTGCGGCAGGCATTGCCGAACCCGACCCTGTTTGCATCACTGTTGA
AAGTGGGCCGGACTGTCCGCCCCTTGCTGCCAAATGGTCTGAAGGATTCGATCCCTGCTGCCCCCATGGAAGAAAGCCGA
CCCTTTCACGCTGCTCGGCACGAGCGCAGGATGCTCGTGCTCCAGGGCTGCGTTCAACCGGCACTGGCGCCCAATATCAA
TGCTGCTGCCGCACGCGTGCTGGACAAGCTCGGCATATCACTTATCAGCGCTCCGGATGCGGGGTGTTGTGGGGCGGTGT
CTTACCATCTCAATGCCCAGAGGGAAGGATTGGATTACATGCGCCGCAACGTGGATGCGTGGTGGCCCTATATCGCGCCC
AAGCCTGAAAAAGAGCCGGGAATGAAGCCTGGGACAAAGGGACGGGCAACAGAAGGAGCAAAGGAGGAAACAAGGGGCGT
CGAAGCAATCGTCATGACTGCGAGCGGCTGCGGCGCGACCGTGGAAGAATACGGTCATCTTTTGCGCCATGATCCCGCAT
ACGCGGAAAAAGCCGCGCGCATCTCGGAATTGACCAGGGACATCAGCGAGATCGTGGAAGCGGAGATGCCGGCGCTCGTT
TCTCTTCTCAGTAAATCGACTGCCAGCAGGGAAAACCCCAGGCAGAAGCTCGCGTTTCATTCTCCCTGCACTTTACAGCA
CGGAATGCGCATCCGGGGTGTCGCGGAGAAGATTCTGGCTGCTGCCGGCTTTGATTTGACCTATGTGCCGGATGCGCATC
TTTGCTGCGGCTCCGCGGGAACCTATTCGATTCTGCAGCCGGAGTTATCGCAGCAGTTGCTGAAAAACAAGGTAACGGCA
CTCGAATCGGGCCATCCTGATCGGATTGCCACTGCCAATATAGGTTGCCTCATGCATATCCGCAGCGGAGCCGCGCTGCC
GGTCGAGCATTGGATCGAGATTTTGGACGAGACCCTTGAGGCCGCTTGGCCTGGCACGATTGAAGATTAG

Upstream 100 bases:

>100_bases
TCCGCTTCCGCCGGCCATGATGAATATTCACCGCCAGCTCAAGGAGAAATTTGACCCGATGCGTATTTTCAATCGGGGCC
GTCTATATCCCGAACTATAA

Downstream 100 bases:

>100_bases
CTTTTCAATGCTTCTGCCAGGAGCTTCTTTCGCGCGCGCTCAGCGCAGCAAGCCGGATTCCCGATTCATATTTTTATCGC
CTTCACCCTGAGTCCCTTGT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MQTNLADFIKDTPEGKEAEAILRTCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQMLEGEPVTEKTQLHLDRCLTCRA
CETACPSGVRYGHLVDIGRGLVEKKVGRSLAAGSMRYVLRQALPNPTLFASLLKVGRTVRPLLPNGLKDSIPAAPMEESR
PFHAARHERRMLVLQGCVQPALAPNINAAAARVLDKLGISLISAPDAGCCGAVSYHLNAQREGLDYMRRNVDAWWPYIAP
KPEKEPGMKPGTKGRATEGAKEETRGVEAIVMTASGCGATVEEYGHLLRHDPAYAEKAARISELTRDISEIVEAEMPALV
SLLSKSTASRENPRQKLAFHSPCTLQHGMRIRGVAEKILAAAGFDLTYVPDAHLCCGSAGTYSILQPELSQQLLKNKVTA
LESGHPDRIATANIGCLMHIRSGAALPVEHWIEILDETLEAAWPGTIED

Sequences:

>Translated_449_residues
MQTNLADFIKDTPEGKEAEAILRTCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQMLEGEPVTEKTQLHLDRCLTCRA
CETACPSGVRYGHLVDIGRGLVEKKVGRSLAAGSMRYVLRQALPNPTLFASLLKVGRTVRPLLPNGLKDSIPAAPMEESR
PFHAARHERRMLVLQGCVQPALAPNINAAAARVLDKLGISLISAPDAGCCGAVSYHLNAQREGLDYMRRNVDAWWPYIAP
KPEKEPGMKPGTKGRATEGAKEETRGVEAIVMTASGCGATVEEYGHLLRHDPAYAEKAARISELTRDISEIVEAEMPALV
SLLSKSTASRENPRQKLAFHSPCTLQHGMRIRGVAEKILAAAGFDLTYVPDAHLCCGSAGTYSILQPELSQQLLKNKVTA
LESGHPDRIATANIGCLMHIRSGAALPVEHWIEILDETLEAAWPGTIED
>Mature_449_residues
MQTNLADFIKDTPEGKEAEAILRTCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQMLEGEPVTEKTQLHLDRCLTCRA
CETACPSGVRYGHLVDIGRGLVEKKVGRSLAAGSMRYVLRQALPNPTLFASLLKVGRTVRPLLPNGLKDSIPAAPMEESR
PFHAARHERRMLVLQGCVQPALAPNINAAAARVLDKLGISLISAPDAGCCGAVSYHLNAQREGLDYMRRNVDAWWPYIAP
KPEKEPGMKPGTKGRATEGAKEETRGVEAIVMTASGCGATVEEYGHLLRHDPAYAEKAARISELTRDISEIVEAEMPALV
SLLSKSTASRENPRQKLAFHSPCTLQHGMRIRGVAEKILAAAGFDLTYVPDAHLCCGSAGTYSILQPELSQQLLKNKVTA
LESGHPDRIATANIGCLMHIRSGAALPVEHWIEILDETLEAAWPGTIED

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=440, Percent_Identity=52.5, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786497, Length=265, Percent_Identity=22.6415094339623, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 48856; Mature: 48856

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQTNLADFIKDTPEGKEAEAILRTCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQMLE
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHC
GEPVTEKTQLHLDRCLTCRACETACPSGVRYGHLVDIGRGLVEKKVGRSLAAGSMRYVLR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALPNPTLFASLLKVGRTVRPLLPNGLKDSIPAAPMEESRPFHAARHERRMLVLQGCVQP
HHCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALAPNINAAAARVLDKLGISLISAPDAGCCGAVSYHLNAQREGLDYMRRNVDAWWPYIAP
HCCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHCCCCCC
KPEKEPGMKPGTKGRATEGAKEETRGVEAIVMTASGCGATVEEYGHLLRHDPAYAEKAAR
CCCCCCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHH
ISELTRDISEIVEAEMPALVSLLSKSTASRENPRQKLAFHSPCTLQHGMRIRGVAEKILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHCCCHHHHHHHHHHH
AAGFDLTYVPDAHLCCGSAGTYSILQPELSQQLLKNKVTALESGHPDRIATANIGCLMHI
HCCCCEEECCCCHHHCCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEE
RSGAALPVEHWIEILDETLEAAWPGTIED
CCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]