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The map label for this gene is purD [H]

Identifier: 82701269

GI number: 82701269

Start: 139749

End: 141056

Strand: Direct

Name: purD [H]

Synonym: Nmul_A0134

Alternate gene names: 82701269

Gene position: 139749-141056 (Clockwise)

Preceding gene: 82701268

Following gene: 82701270

Centisome position: 4.39

GC content: 57.95

Gene sequence:

>1308_bases
ATGAAACTACTTGTAATCGGTGGTGGTGCCAGAGAACACGCCCTCGCGTGGAAGCTGCTGGATTCCTCGCGCAGAAGACT
TCCTGCGACCGTATTTGTCGCCCCGGGCAATGCGGGTACCGAGCTCGAAAAAGACCTGAAGAACGTGCCCATCACCACCA
TCCCGGAGTTGATCGAATTCGCCAGAAAGGAATCGATCGCGTTCACTGTTGTGGGGCCGGAAGCCCCCCTGGCGGAAGGT
ATCGTCGATGCCTTCCGTTTCGCTGGCCTGAAGATCTTCGGTCCTACCCAAAAGGCAGCACAACTGGAAACTTCCAAGGA
TTTCGCCAAGGCTTTCATGGGCCGCCATGGCATTCCTACCGCAGACTATGCCACCTTCAGTTCCGCTGCCAAGGCGCACC
GGTATATCGACAAGAAGGGCGTTCCCATCGTGATAAAAGCGGACGGCCTGGCGGCAGGCAAAGGCGTGGTCGTGGCGGTG
ACGCTGGAAGAAGCGCATGCCGCCGTGGACAAGATGCTGGGGGGAAAGTCGGGCAAGGCAGGACCGAAGATCGTGATCGA
AGAATTCGTGGAAGGCGAAGAAATCAGTTTTATCGTCATGACTGACGGTCGTCACGTTTTGCCGCTGGCCAGCAGTCAGG
ATCACAAGCGCCTGAAGGATGGCGACGAGGGACCTAACACCGGAGGCATGGGGGCCTACTCGCCCGCTTCCTCAATTACC
CCCACTCTCCACGGGAAAATAATGCGGGAAGTCATCCAGCGCGCGATAAGCGGGATGGAAAAGGAAGGCGAGCGCTACAT
CGGTTTCCTTTATGCCGGGCTCATGATTACCCCAGACGGGGGCATAAAAGTGCTCGAGTTCAATTGCCGCATGGGCGATC
CGGAAGCCGAGGTTATCCTGCTGCGCCTCAAGAGCAATTTTATCACCCTCATCGAGCATGCCCTGTCCGGGAATCTGGAC
AAGATAGAGGCCGAGTGGGACCGGCGCGCTGCTCTGGCGGTGGTTATGGCAGCGAGTGGATACCCGGATTCTCCCCGCAA
GGGCGATGCCATCGAAGGGCTGTCCGCCTTGCCTGCAACGTTGCCTGTGGAGAGAGACTATCATGTTTTCCATGCGGGAA
CCGCCCGGGGCGGCGAAAACGGGGAAGAGATCGTGACGGCCGGTGGGCGCATTCTCTGTGTGGCTGCCTTGGGCGACAAC
TTCAGGATGGCTCAGCGACGTGCTTATGAAATCGCCGAGCAGATTCACTTCGACGGTTGCCAGATGCGACGTGATATCGG
GTATCGGGCTATTAACCACCGTAAATAG

Upstream 100 bases:

>100_bases
TTTCTATGAATCCCTCGATCAACCCAACCCATTATCCGCTAGAGGCGGATAGCACAGCCCGATTCGCAAGGGGACTCCTT
GCAGTTCTGAATCCAGAGAT

Downstream 100 bases:

>100_bases
CGGTGGACGGGGTGGAAGAAAAAACTCAGCAGCAAACAGGTGTATGGGAGATCGGATGAGCACTGCACAACAAGCCCGGG
AGTTCTTTACGGGACTGCAG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKLLVIGGGAREHALAWKLLDSSRRRLPATVFVAPGNAGTELEKDLKNVPITTIPELIEFARKESIAFTVVGPEAPLAEG
IVDAFRFAGLKIFGPTQKAAQLETSKDFAKAFMGRHGIPTADYATFSSAAKAHRYIDKKGVPIVIKADGLAAGKGVVVAV
TLEEAHAAVDKMLGGKSGKAGPKIVIEEFVEGEEISFIVMTDGRHVLPLASSQDHKRLKDGDEGPNTGGMGAYSPASSIT
PTLHGKIMREVIQRAISGMEKEGERYIGFLYAGLMITPDGGIKVLEFNCRMGDPEAEVILLRLKSNFITLIEHALSGNLD
KIEAEWDRRAALAVVMAASGYPDSPRKGDAIEGLSALPATLPVERDYHVFHAGTARGGENGEEIVTAGGRILCVAALGDN
FRMAQRRAYEIAEQIHFDGCQMRRDIGYRAINHRK

Sequences:

>Translated_435_residues
MKLLVIGGGAREHALAWKLLDSSRRRLPATVFVAPGNAGTELEKDLKNVPITTIPELIEFARKESIAFTVVGPEAPLAEG
IVDAFRFAGLKIFGPTQKAAQLETSKDFAKAFMGRHGIPTADYATFSSAAKAHRYIDKKGVPIVIKADGLAAGKGVVVAV
TLEEAHAAVDKMLGGKSGKAGPKIVIEEFVEGEEISFIVMTDGRHVLPLASSQDHKRLKDGDEGPNTGGMGAYSPASSIT
PTLHGKIMREVIQRAISGMEKEGERYIGFLYAGLMITPDGGIKVLEFNCRMGDPEAEVILLRLKSNFITLIEHALSGNLD
KIEAEWDRRAALAVVMAASGYPDSPRKGDAIEGLSALPATLPVERDYHVFHAGTARGGENGEEIVTAGGRILCVAALGDN
FRMAQRRAYEIAEQIHFDGCQMRRDIGYRAINHRK
>Mature_435_residues
MKLLVIGGGAREHALAWKLLDSSRRRLPATVFVAPGNAGTELEKDLKNVPITTIPELIEFARKESIAFTVVGPEAPLAEG
IVDAFRFAGLKIFGPTQKAAQLETSKDFAKAFMGRHGIPTADYATFSSAAKAHRYIDKKGVPIVIKADGLAAGKGVVVAV
TLEEAHAAVDKMLGGKSGKAGPKIVIEEFVEGEEISFIVMTDGRHVLPLASSQDHKRLKDGDEGPNTGGMGAYSPASSIT
PTLHGKIMREVIQRAISGMEKEGERYIGFLYAGLMITPDGGIKVLEFNCRMGDPEAEVILLRLKSNFITLIEHALSGNLD
KIEAEWDRRAALAVVMAASGYPDSPRKGDAIEGLSALPATLPVERDYHVFHAGTARGGENGEEIVTAGGRILCVAALGDN
FRMAQRRAYEIAEQIHFDGCQMRRDIGYRAINHRK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=435, Percent_Identity=48.5057471264368, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI209869995, Length=435, Percent_Identity=48.5057471264368, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI209869993, Length=435, Percent_Identity=48.5057471264368, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI28416899, Length=435, Percent_Identity=48.5057471264368, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1790438, Length=440, Percent_Identity=57.5, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17567511, Length=432, Percent_Identity=38.8888888888889, Blast_Score=281, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321203, Length=461, Percent_Identity=38.3947939262473, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI62471619, Length=441, Percent_Identity=44.4444444444444, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24582400, Length=440, Percent_Identity=44.5454545454545, Blast_Score=316, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46866; Mature: 46866

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVIGGGAREHALAWKLLDSSRRRLPATVFVAPGNAGTELEKDLKNVPITTIPELIEF
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHH
ARKESIAFTVVGPEAPLAEGIVDAFRFAGLKIFGPTQKAAQLETSKDFAKAFMGRHGIPT
HHHCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
ADYATFSSAAKAHRYIDKKGVPIVIKADGLAAGKGVVVAVTLEEAHAAVDKMLGGKSGKA
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
GPKIVIEEFVEGEEISFIVMTDGRHVLPLASSQDHKRLKDGDEGPNTGGMGAYSPASSIT
CCHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
PTLHGKIMREVIQRAISGMEKEGERYIGFLYAGLMITPDGGIKVLEFNCRMGDPEAEVIL
HHHHHHHHHHHHHHHHCCCHHCCHHEEEEEEECEEECCCCCEEEEEEECCCCCCCCEEEE
LRLKSNFITLIEHALSGNLDKIEAEWDRRAALAVVMAASGYPDSPRKGDAIEGLSALPAT
EEECCHHHHHHHHHHCCCCCEEHHHHHHHHEEEEEEEECCCCCCCCCCCHHCCHHHCCCC
LPVERDYHVFHAGTARGGENGEEIVTAGGRILCVAALGDNFRMAQRRAYEIAEQIHFDGC
CCCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHH
QMRRDIGYRAINHRK
HHHHHCCCCHHCCCC
>Mature Secondary Structure
MKLLVIGGGAREHALAWKLLDSSRRRLPATVFVAPGNAGTELEKDLKNVPITTIPELIEF
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHH
ARKESIAFTVVGPEAPLAEGIVDAFRFAGLKIFGPTQKAAQLETSKDFAKAFMGRHGIPT
HHHCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
ADYATFSSAAKAHRYIDKKGVPIVIKADGLAAGKGVVVAVTLEEAHAAVDKMLGGKSGKA
CCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
GPKIVIEEFVEGEEISFIVMTDGRHVLPLASSQDHKRLKDGDEGPNTGGMGAYSPASSIT
CCHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
PTLHGKIMREVIQRAISGMEKEGERYIGFLYAGLMITPDGGIKVLEFNCRMGDPEAEVIL
HHHHHHHHHHHHHHHHCCCHHCCHHEEEEEEECEEECCCCCEEEEEEECCCCCCCCEEEE
LRLKSNFITLIEHALSGNLDKIEAEWDRRAALAVVMAASGYPDSPRKGDAIEGLSALPAT
EEECCHHHHHHHHHHCCCCCEEHHHHHHHHEEEEEEEECCCCCCCCCCCHHCCHHHCCCC
LPVERDYHVFHAGTARGGENGEEIVTAGGRILCVAALGDNFRMAQRRAYEIAEQIHFDGC
CCCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHH
QMRRDIGYRAINHRK
HHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]