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The map label for this gene is purH [H]

Identifier: 82701268

GI number: 82701268

Start: 137927

End: 139489

Strand: Direct

Name: purH [H]

Synonym: Nmul_A0133

Alternate gene names: 82701268

Gene position: 137927-139489 (Clockwise)

Preceding gene: 82701267

Following gene: 82701269

Centisome position: 4.33

GC content: 58.99

Gene sequence:

>1563_bases
ATGTCGATTAAACAGGCCCTGATCAGTGTTTCAGATAAAAGCGGTATTGTCGAGTTTGCTCAAGCGCTGCACAAACTTGG
AGTAACCATTCTGTCAACGGGCGGCACCGCCAGACTTTTGAGGGATGCCGGCGTTGCCGTGACCGAGGTCGGGAGCTACA
CCGGCTTTCCCGAGATGCTGGATGGGCGGGTCAAGACCCTGCATCCAAAAATACACGCGGGAATTCTGGCCAGACGGGAT
TTGTCCGAGCATGTGTCCGCGCTGGAAAAGGCGGAAATTCCTGCCATCGATCTGGTAGTAGTGAATCTCTATCCTTTCAG
CCAGACAGTGGCGCAGCCGGATTGCAGTCTGGAAGAGGCCATTGAAAATATCGATATCGGCGGCCCGACCATGGTGCGCG
CTGCGGCCAAGAACTACCAGAGCGTGGCCGTAGTCACCGATCCGGCGGATTACCCCGCGTTACTTGACGAGATGAAGACT
GCCGGCGGCGAGGTTACGCCTGAGTTCCGGTTCCGGCTGGCGTGCAAGGCGTTTTCGCATACAGCCGCCTATGATGGGGC
GATCAGCAACTACCTCACCTCCATCGAGGGGGAAAATGCACAACGTCGTACCTTTCCGGAACGCCTGAATCTCAATTTCA
GCCTGGTGCAGCCTCTGCGCTACGGGGAAAACCCGCATCAGCAGGCCGCCTTTTACCGCGATCCGCAACTCGTTCCCGGC
AGCCTTGCAAGCTACAGGCAGTTGCAGGGCAAGGAGCTCTCTTACAACAACATCGCGGACGCGGATGCCGCCTGGGAATG
CGTAAAGACGTTTGATTCCCCGGCCTGTGTCATCATCAAGCATGCCAATCCTTGCGGCGTGGCAATCAGCGATTCGCCGC
TCGCGGCTTACAAGCTTGCATTTGCCACCGATCCCACTTCCGCATTCGGCGGCATTATCGCTTTCAATCGCACGCTGGAC
GCCTCGGCCGCCGAGGCGGTGATGAGCCAGTTTGTCGAAGTGATCATCGCGCCGCAAATGACCGACGAGGCAAGGCAGAT
GCTCGCGCGCAAAGCCAATGTGCGCGTGCTGACCGTGCCGCTCCAGGCGGGGAACAACGCCCACGACTTCAAGCGAGTGG
GTGGAGGATTGCTGGTGCAGACTCCGGACAATCTCAACGTTACCCCTAACCAATTGAAGGTGGTGACCGAAGTCCAGCCA
ACAGCGCAGCAATTGCAGGACCTGCTGTTTGCCTGGCGGGTGGCGAAATTCGTCAAATCGAATGCCATCGTCTTTTGTGC
CAACGGCCGCACGCTTGGCGTGGGCGCCGGACAGATGAGCAGGGTGGATAGCGCGCGCATTGCCTCCATCAAAGCCGGGA
ACGCGAACCTCACCCTGGCAGGCTCGGTAGTGGCATCCGATGCCTTCTTCCCTTTCCGCGACGGACTGGATGTCGTCGTC
CAGGCGGGGGCGGTGGCGGTCATCCAGCCGGGGGGCAGTGTGCGGGATGAAGAGGTTATCGCTGCGGCGGATGAACAAGG
GGTGGCAATGGTATTTACCGGCGTGCGCCATTTCAGGCATTGA

Upstream 100 bases:

>100_bases
CAAAATTGTCGTTACGCGCCTGAAGGTCAGCCGCAAGCGGGCCAATTAACGGTTGTAATGCATCACGCTACAGACCCATC
CTGATTCAATAAAAATACGA

Downstream 100 bases:

>100_bases
ATTCAAGCATTGAATGCATGGAAAAAGCGGTTGTTCAATGCCGGCCGAGCCACCTCTCCCATACCCGAACCCCGCATAGC
CCTCCTTTTGGGTCGATAGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSIKQALISVSDKSGIVEFAQALHKLGVTILSTGGTARLLRDAGVAVTEVGSYTGFPEMLDGRVKTLHPKIHAGILARRD
LSEHVSALEKAEIPAIDLVVVNLYPFSQTVAQPDCSLEEAIENIDIGGPTMVRAAAKNYQSVAVVTDPADYPALLDEMKT
AGGEVTPEFRFRLACKAFSHTAAYDGAISNYLTSIEGENAQRRTFPERLNLNFSLVQPLRYGENPHQQAAFYRDPQLVPG
SLASYRQLQGKELSYNNIADADAAWECVKTFDSPACVIIKHANPCGVAISDSPLAAYKLAFATDPTSAFGGIIAFNRTLD
ASAAEAVMSQFVEVIIAPQMTDEARQMLARKANVRVLTVPLQAGNNAHDFKRVGGGLLVQTPDNLNVTPNQLKVVTEVQP
TAQQLQDLLFAWRVAKFVKSNAIVFCANGRTLGVGAGQMSRVDSARIASIKAGNANLTLAGSVVASDAFFPFRDGLDVVV
QAGAVAVIQPGGSVRDEEVIAAADEQGVAMVFTGVRHFRH

Sequences:

>Translated_520_residues
MSIKQALISVSDKSGIVEFAQALHKLGVTILSTGGTARLLRDAGVAVTEVGSYTGFPEMLDGRVKTLHPKIHAGILARRD
LSEHVSALEKAEIPAIDLVVVNLYPFSQTVAQPDCSLEEAIENIDIGGPTMVRAAAKNYQSVAVVTDPADYPALLDEMKT
AGGEVTPEFRFRLACKAFSHTAAYDGAISNYLTSIEGENAQRRTFPERLNLNFSLVQPLRYGENPHQQAAFYRDPQLVPG
SLASYRQLQGKELSYNNIADADAAWECVKTFDSPACVIIKHANPCGVAISDSPLAAYKLAFATDPTSAFGGIIAFNRTLD
ASAAEAVMSQFVEVIIAPQMTDEARQMLARKANVRVLTVPLQAGNNAHDFKRVGGGLLVQTPDNLNVTPNQLKVVTEVQP
TAQQLQDLLFAWRVAKFVKSNAIVFCANGRTLGVGAGQMSRVDSARIASIKAGNANLTLAGSVVASDAFFPFRDGLDVVV
QAGAVAVIQPGGSVRDEEVIAAADEQGVAMVFTGVRHFRH
>Mature_519_residues
SIKQALISVSDKSGIVEFAQALHKLGVTILSTGGTARLLRDAGVAVTEVGSYTGFPEMLDGRVKTLHPKIHAGILARRDL
SEHVSALEKAEIPAIDLVVVNLYPFSQTVAQPDCSLEEAIENIDIGGPTMVRAAAKNYQSVAVVTDPADYPALLDEMKTA
GGEVTPEFRFRLACKAFSHTAAYDGAISNYLTSIEGENAQRRTFPERLNLNFSLVQPLRYGENPHQQAAFYRDPQLVPGS
LASYRQLQGKELSYNNIADADAAWECVKTFDSPACVIIKHANPCGVAISDSPLAAYKLAFATDPTSAFGGIIAFNRTLDA
SAAEAVMSQFVEVIIAPQMTDEARQMLARKANVRVLTVPLQAGNNAHDFKRVGGGLLVQTPDNLNVTPNQLKVVTEVQPT
AQQLQDLLFAWRVAKFVKSNAIVFCANGRTLGVGAGQMSRVDSARIASIKAGNANLTLAGSVVASDAFFPFRDGLDVVVQ
AGAVAVIQPGGSVRDEEVIAAADEQGVAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=38.2845188284519, Blast_Score=275, Evalue=6e-74,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=61.4800759013283, Blast_Score=630, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=518, Percent_Identity=35.7142857142857, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71985574, Length=348, Percent_Identity=26.7241379310345, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=34.453781512605, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.4583333333333, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=484, Percent_Identity=36.5702479338843, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24649832, Length=488, Percent_Identity=37.2950819672131, Blast_Score=263, Evalue=3e-70,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55636; Mature: 55505

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKQALISVSDKSGIVEFAQALHKLGVTILSTGGTARLLRDAGVAVTEVGSYTGFPEML
CCHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEECCCCCCCHHHH
DGRVKTLHPKIHAGILARRDLSEHVSALEKAEIPAIDLVVVNLYPFSQTVAQPDCSLEEA
CCCHHEECCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHH
IENIDIGGPTMVRAAAKNYQSVAVVTDPADYPALLDEMKTAGGEVTPEFRFRLACKAFSH
HHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHEEHHHHHHH
TAAYDGAISNYLTSIEGENAQRRTFPERLNLNFSLVQPLRYGENPHQQAAFYRDPQLVPG
HHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCEEECHHHCCCCHHHHHHHCCCCCCCCC
SLASYRQLQGKELSYNNIADADAAWECVKTFDSPACVIIKHANPCGVAISDSPLAAYKLA
CHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCEEEEEE
FATDPTSAFGGIIAFNRTLDASAAEAVMSQFVEVIIAPQMTDEARQMLARKANVRVLTVP
EECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEE
LQAGNNAHDFKRVGGGLLVQTPDNLNVTPNQLKVVTEVQPTAQQLQDLLFAWRVAKFVKS
ECCCCCHHHHHHHCCCEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NAIVFCANGRTLGVGAGQMSRVDSARIASIKAGNANLTLAGSVVASDAFFPFRDGLDVVV
CCEEEEECCCEEEECCCHHHHCCCHHEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHEE
QAGAVAVIQPGGSVRDEEVIAAADEQGVAMVFTGVRHFRH
ECCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure 
SIKQALISVSDKSGIVEFAQALHKLGVTILSTGGTARLLRDAGVAVTEVGSYTGFPEML
CHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEECCCCCCCHHHH
DGRVKTLHPKIHAGILARRDLSEHVSALEKAEIPAIDLVVVNLYPFSQTVAQPDCSLEEA
CCCHHEECCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEEECCHHHHCCCCCCHHHH
IENIDIGGPTMVRAAAKNYQSVAVVTDPADYPALLDEMKTAGGEVTPEFRFRLACKAFSH
HHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHEEHHHHHHH
TAAYDGAISNYLTSIEGENAQRRTFPERLNLNFSLVQPLRYGENPHQQAAFYRDPQLVPG
HHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCEEECHHHCCCCHHHHHHHCCCCCCCCC
SLASYRQLQGKELSYNNIADADAAWECVKTFDSPACVIIKHANPCGVAISDSPLAAYKLA
CHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCEEEEEE
FATDPTSAFGGIIAFNRTLDASAAEAVMSQFVEVIIAPQMTDEARQMLARKANVRVLTVP
EECCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEE
LQAGNNAHDFKRVGGGLLVQTPDNLNVTPNQLKVVTEVQPTAQQLQDLLFAWRVAKFVKS
ECCCCCHHHHHHHCCCEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHC
NAIVFCANGRTLGVGAGQMSRVDSARIASIKAGNANLTLAGSVVASDAFFPFRDGLDVVV
CCEEEEECCCEEEECCCHHHHCCCHHEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHEE
QAGAVAVIQPGGSVRDEEVIAAADEQGVAMVFTGVRHFRH
ECCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA