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The map label for this gene is rmlA1 [C]

Identifier: 82701262

GI number: 82701262

Start: 132372

End: 133076

Strand: Direct

Name: rmlA1 [C]

Synonym: Nmul_A0127

Alternate gene names: 82701262

Gene position: 132372-133076 (Clockwise)

Preceding gene: 82701261

Following gene: 82701263

Centisome position: 4.16

GC content: 56.88

Gene sequence:

>705_bases
ATGATCCTCGCGGCGGGACGTGGAGAACGCATGCGTCCCCTGACCGATACTTTGCCCAAGCCTTTACTGCGCGTGGGAGG
CAAGGCGCTGATCGAATATCACCTGCAAAATCTCGCACGAGCCGGGTTTCACGATATTGTCATCAATCACGCTCATCTGG
GACAGATGATCGAAGCCGCGCTGGGTAATGGCGAGCGCTTCGGGCTCCGCATTCACTACTCGCCCGAACCGGTTGCGCTG
GAAACCGCGGGTGGAATTGCGCAAGCCCTGCCTATCATAAGAGCGGGCTTTGGCAAAGGGCGTCGACCTTTTCTTGCCGT
GAATGCCGATATCTATTGCAGCATGGACTTCTCAATTCTGCTGCCGGTCTTGCACCGCCTGCGGGACCATCCGGACAGCG
CGCTTGCGCATCTGGTGCTGGTCGATAATCCTGCGCATCACCCGAACGGGGATTTTTCTCTCGAAGCCGGCAGGGTTGCA
GTAACCGGAAAAGAAATGCTTACGTTCAGTGGCATTGGCATATATCAGCCCCAGCTTTTTCAGGATATTGTGCCCGGAAC
CGTGGCTAAACTCGCTCCATTGTTGAAGGAGGCCATGGCCGGAGAAAAAGTGAGCGGACAACGCTATGCCGGGTCATGGG
TAGATGTGGGTACTCCGGAACGTCTTCGCCTGCTCGACATCGATCTGATGCGGCCGGTGTCCTGA

Upstream 100 bases:

>100_bases
CTCAATCCCTTGCTGGCGCTGCTTGACAGGTGGGAGAGCAACAAGCCGGGACTGAAAGTTGGCTATACTTTCTGAGAAGG
CGGGAAACGGGTTCACGGCC

Downstream 100 bases:

>100_bases
ACTGCCTCCTGCCACCTCCCGGCGCTCCGGAATCAGGCGGGAGCGGGATCAGGGAAAAGAATGGGCCGATTTACTTTGCT
TTATCATTCAGATACTGTAA

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Nucleotidyltransferase Family Protein; Nucleoside-Diphosphate-Sugar Pyrophosphorylase; Nucleotidyl Transferase Family Protein; Mannose-1-Phosphate Guanylyltransferase; Nucleotidyltransferase; Sugar-Phosphate Nucleotidyl Transferase; Mannose-1-Phosphate Guanyltransferase; Nucleotidyl Transferase Superfamily; Nucleotidyl Transferase Protein; Mannose-1-Phosphate Guanyltransferase-Related Protein; Nucleotidyltransferase Protein; Transaldolase AB; ADP-Glucose Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase Protein; SMC Domain-Containing Protein; Nucleotidyl Transferase Superfamily Protein; NDP-Sugar Synthase; HAD-Superfamily Protein; Polysaccharide Biosynthesis Protein; Sugar-Phosphate Nucleotide Transferase; Glucose-1-Phosphate Nucleotidyltransferase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MILAAGRGERMRPLTDTLPKPLLRVGGKALIEYHLQNLARAGFHDIVINHAHLGQMIEAALGNGERFGLRIHYSPEPVAL
ETAGGIAQALPIIRAGFGKGRRPFLAVNADIYCSMDFSILLPVLHRLRDHPDSALAHLVLVDNPAHHPNGDFSLEAGRVA
VTGKEMLTFSGIGIYQPQLFQDIVPGTVAKLAPLLKEAMAGEKVSGQRYAGSWVDVGTPERLRLLDIDLMRPVS

Sequences:

>Translated_234_residues
MILAAGRGERMRPLTDTLPKPLLRVGGKALIEYHLQNLARAGFHDIVINHAHLGQMIEAALGNGERFGLRIHYSPEPVAL
ETAGGIAQALPIIRAGFGKGRRPFLAVNADIYCSMDFSILLPVLHRLRDHPDSALAHLVLVDNPAHHPNGDFSLEAGRVA
VTGKEMLTFSGIGIYQPQLFQDIVPGTVAKLAPLLKEAMAGEKVSGQRYAGSWVDVGTPERLRLLDIDLMRPVS
>Mature_234_residues
MILAAGRGERMRPLTDTLPKPLLRVGGKALIEYHLQNLARAGFHDIVINHAHLGQMIEAALGNGERFGLRIHYSPEPVAL
ETAGGIAQALPIIRAGFGKGRRPFLAVNADIYCSMDFSILLPVLHRLRDHPDSALAHLVLVDNPAHHPNGDFSLEAGRVA
VTGKEMLTFSGIGIYQPQLFQDIVPGTVAKLAPLLKEAMAGEKVSGQRYAGSWVDVGTPERLRLLDIDLMRPVS

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11761621, Length=233, Percent_Identity=28.3261802575107, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI11761619, Length=233, Percent_Identity=28.3261802575107, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=231, Percent_Identity=25.974025974026, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355443, Length=231, Percent_Identity=25.5411255411255, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24644084, Length=231, Percent_Identity=25.5411255411255, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 25410; Mature: 25410

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAAGRGERMRPLTDTLPKPLLRVGGKALIEYHLQNLARAGFHDIVINHAHLGQMIEAA
CEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHH
LGNGERFGLRIHYSPEPVALETAGGIAQALPIIRAGFGKGRRPFLAVNADIYCSMDFSIL
HCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCEEEEECCHHHH
LPVLHRLRDHPDSALAHLVLVDNPAHHPNGDFSLEAGRVAVTGKEMLTFSGIGIYQPQLF
HHHHHHHHCCCCHHHEEEEEECCCCCCCCCCEEEECCEEEEECCCEEEECCCCCCCHHHH
QDIVPGTVAKLAPLLKEAMAGEKVSGQRYAGSWVDVGTPERLRLLDIDLMRPVS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEEHHCCCCC
>Mature Secondary Structure
MILAAGRGERMRPLTDTLPKPLLRVGGKALIEYHLQNLARAGFHDIVINHAHLGQMIEAA
CEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHH
LGNGERFGLRIHYSPEPVALETAGGIAQALPIIRAGFGKGRRPFLAVNADIYCSMDFSIL
HCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEECCEEEEECCHHHH
LPVLHRLRDHPDSALAHLVLVDNPAHHPNGDFSLEAGRVAVTGKEMLTFSGIGIYQPQLF
HHHHHHHHCCCCHHHEEEEEECCCCCCCCCCEEEECCEEEEECCCEEEECCCCCCCHHHH
QDIVPGTVAKLAPLLKEAMAGEKVSGQRYAGSWVDVGTPERLRLLDIDLMRPVS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA