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The map label for this gene is thiG

Identifier: 82701205

GI number: 82701205

Start: 75617

End: 76411

Strand: Reverse

Name: thiG

Synonym: Nmul_A0070

Alternate gene names: 82701205

Gene position: 76411-75617 (Counterclockwise)

Preceding gene: 82701206

Following gene: 82701204

Centisome position: 2.4

GC content: 56.1

Gene sequence:

>795_bases
ATGGACAAACTTGTCATCGCAGGTAAAGCTTATTCCTCACGCCTCCTGGTGGGTACCGGAAAATATAGGGATTTTGACGA
GACCCGCACCGCCGTGGACGCCAGCGGCGCAGAAATCATTACCGTGGCTATCCGCAGAACGAATCTCGGCCAGAACCCGG
AAGAGCCCAGTCTGCTGGATGTGCTGCCGCCATCGGAATATACGCTGTTACCCAATACAGCCGGATGCTATACGGTGGAG
GATGCAGTAAGAACCTTGCGCCTTGCGCGCGAATTGCTGGACGGTCATACCCTGGTGAAGCTGGAGGTCCTGGGTGATCC
GAAAACACTCTATCCCAACATCGTTGAAACCCTCAAGGCAGCCGAGATTCTGATCAAGGAGGGTTTCCAGGTCATGGTCT
ATACCTCGGATGACCCTATTGCCGCCAGGCAGCTGGAAGAGATCGGCTGCGTCGCGATCATGCCGCTGGCATCCCTGATC
GGTTCGGGCATGGGTATCCTCAATCCATGGAACCTGCAGATCATCATCGAAAATGCCAAAGTCCCGGTCATTGTGGATGC
TGGTGTGGGTACGGCATCGGATGCGGCGATTGCAATGGAACTGGGGTGTGACGGCGTGCTCATGAACACGGCCATTGCTG
CCGCGCAAAATCCGGTGCTGATGGCATCAGCGATGAAAAAAGCGGTTGAAGCGGGGCGCGAAGCGTATCTCGCTGGCCGG
ATGCCGAAAAAGCTGTACAGTGCAAGTCCCAGCTCCCCGGTGGAGGGAGTTATCGGCAACATGTCGGGGAAATAA

Upstream 100 bases:

>100_bases
GCGCAACGGTGAAATCGTACCGCGCAGCCAGTTCGATCAGCCGATGCTTGCAAGCGGCGACAAGCTTGAAATTGTCGTTG
CCGTAGGTGGAGGGTAGCCC

Downstream 100 bases:

>100_bases
GGACCGGGAAAGATTTCGTATTCCCCCACCTGTCCTTTCCAGGCATATCAAATGGTTGAACATTCTCCCCATCGTCCCAT
CCGCAGCTTCGTTCTTCGCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDKLVIAGKAYSSRLLVGTGKYRDFDETRTAVDASGAEIITVAIRRTNLGQNPEEPSLLDVLPPSEYTLLPNTAGCYTVE
DAVRTLRLARELLDGHTLVKLEVLGDPKTLYPNIVETLKAAEILIKEGFQVMVYTSDDPIAARQLEEIGCVAIMPLASLI
GSGMGILNPWNLQIIIENAKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPVLMASAMKKAVEAGREAYLAGR
MPKKLYSASPSSPVEGVIGNMSGK

Sequences:

>Translated_264_residues
MDKLVIAGKAYSSRLLVGTGKYRDFDETRTAVDASGAEIITVAIRRTNLGQNPEEPSLLDVLPPSEYTLLPNTAGCYTVE
DAVRTLRLARELLDGHTLVKLEVLGDPKTLYPNIVETLKAAEILIKEGFQVMVYTSDDPIAARQLEEIGCVAIMPLASLI
GSGMGILNPWNLQIIIENAKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPVLMASAMKKAVEAGREAYLAGR
MPKKLYSASPSSPVEGVIGNMSGK
>Mature_264_residues
MDKLVIAGKAYSSRLLVGTGKYRDFDETRTAVDASGAEIITVAIRRTNLGQNPEEPSLLDVLPPSEYTLLPNTAGCYTVE
DAVRTLRLARELLDGHTLVKLEVLGDPKTLYPNIVETLKAAEILIKEGFQVMVYTSDDPIAARQLEEIGCVAIMPLASLI
GSGMGILNPWNLQIIIENAKVPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPVLMASAMKKAVEAGREAYLAGR
MPKKLYSASPSSPVEGVIGNMSGK

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=255, Percent_Identity=45.0980392156863, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_NITMU (Q2YCZ2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410771.1
- ProteinModelPortal:   Q2YCZ2
- SMR:   Q2YCZ2
- STRING:   Q2YCZ2
- GeneID:   3785794
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0070
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q2YCZ2
- ProtClustDB:   PRK00208
- BioCyc:   NMUL323848:NMUL_A0070-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27971; Mature: 27971

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: ACT_SITE 100-100 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLVIAGKAYSSRLLVGTGKYRDFDETRTAVDASGAEIITVAIRRTNLGQNPEEPSLLD
CCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEE
VLPPSEYTLLPNTAGCYTVEDAVRTLRLARELLDGHTLVKLEVLGDPKTLYPNIVETLKA
ECCCCCEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHH
AEILIKEGFQVMVYTSDDPIAARQLEEIGCVAIMPLASLIGSGMGILNPWNLQIIIENAK
HHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
VPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPVLMASAMKKAVEAGREAYLAGR
CCEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCC
MPKKLYSASPSSPVEGVIGNMSGK
CCHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MDKLVIAGKAYSSRLLVGTGKYRDFDETRTAVDASGAEIITVAIRRTNLGQNPEEPSLLD
CCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEE
VLPPSEYTLLPNTAGCYTVEDAVRTLRLARELLDGHTLVKLEVLGDPKTLYPNIVETLKA
ECCCCCEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHH
AEILIKEGFQVMVYTSDDPIAARQLEEIGCVAIMPLASLIGSGMGILNPWNLQIIIENAK
HHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
VPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPVLMASAMKKAVEAGREAYLAGR
CCEEEECCCCCCCCCEEEEEECCCEEHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCC
MPKKLYSASPSSPVEGVIGNMSGK
CCHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA