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The map label for this gene is trmB

Identifier: 82701204

GI number: 82701204

Start: 74876

End: 75565

Strand: Reverse

Name: trmB

Synonym: Nmul_A0069

Alternate gene names: 82701204

Gene position: 75565-74876 (Counterclockwise)

Preceding gene: 82701205

Following gene: 82701200

Centisome position: 2.37

GC content: 57.25

Gene sequence:

>690_bases
ATGGTTGAACATTCTCCCCATCGTCCCATCCGCAGCTTCGTTCTTCGCCAGGGACGCCTTTCAAACGCGCAGCGGCGTGC
CCATGAAACATTGATGCCGAAATACGGCATCCCTTATTCCGGGAAACTGCTTGACCTGGCCATTATCTTCAGCAGAAGTG
CGCCCAAATTCCTGGAAATCGGCTTCGGCATGGGCGAAACCACGGCGCTGATCGCCCAAGCGCATCCGCAAAACGATTAT
CTTGCCGCTGAAGTCCATACGCCCGGCATGGGCAGCCTGCTCAAGCAAGTGGAAGAACTCGAGCTTACGAATATACGCGT
CATCCAGCACGATGCGGTTGATGTCCTGCAACACGCTTTGCCCCCTGAGTGCCTCGACGGGGTGCACGTTTTCTTTCCCG
ACCCGTGGCCCAAGGCACGCCATCACAAGCGCCGGCTGATACAAGCCGAATTCGTTGACCTGCTTTGCAGCCGCCTGAAA
CCCGGCGGCTATATCCATGTGGCGACCGACTGGGAGGATTATGCGGAGCAAATACTGGAAGTATTGAGCGGCGAGCCGCA
CTTGAGCAATACCGCAGTTGGTTATGCGCCACGGCCGGAATATCGTCCCCTCACAAAGTTCGAGCAGCGGGGTCTGCGGC
TCGGACACGAGGTCTGGGATGTGATATTCCGGAAAAAACAGGGCGCCTGA

Upstream 100 bases:

>100_bases
TCCCAGCTCCCCGGTGGAGGGAGTTATCGGCAACATGTCGGGGAAATAAGGACCGGGAAAGATTTCGTATTCCCCCACCT
GTCCTTTCCAGGCATATCAA

Downstream 100 bases:

>100_bases
AGAGGTTTATACGCGGCTTTGCCCGGGAAGGAAAATCTGCAGCAAATCGTTCAAAAAACGCCTGCCTGCGAAAGTAGGCG
CAATACGCTGAAAATCCCGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MVEHSPHRPIRSFVLRQGRLSNAQRRAHETLMPKYGIPYSGKLLDLAIIFSRSAPKFLEIGFGMGETTALIAQAHPQNDY
LAAEVHTPGMGSLLKQVEELELTNIRVIQHDAVDVLQHALPPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLK
PGGYIHVATDWEDYAEQILEVLSGEPHLSNTAVGYAPRPEYRPLTKFEQRGLRLGHEVWDVIFRKKQGA

Sequences:

>Translated_229_residues
MVEHSPHRPIRSFVLRQGRLSNAQRRAHETLMPKYGIPYSGKLLDLAIIFSRSAPKFLEIGFGMGETTALIAQAHPQNDY
LAAEVHTPGMGSLLKQVEELELTNIRVIQHDAVDVLQHALPPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLK
PGGYIHVATDWEDYAEQILEVLSGEPHLSNTAVGYAPRPEYRPLTKFEQRGLRLGHEVWDVIFRKKQGA
>Mature_229_residues
MVEHSPHRPIRSFVLRQGRLSNAQRRAHETLMPKYGIPYSGKLLDLAIIFSRSAPKFLEIGFGMGETTALIAQAHPQNDY
LAAEVHTPGMGSLLKQVEELELTNIRVIQHDAVDVLQHALPPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLK
PGGYIHVATDWEDYAEQILEVLSGEPHLSNTAVGYAPRPEYRPLTKFEQRGLRLGHEVWDVIFRKKQGA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=223, Percent_Identity=51.1210762331839, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NITMU (Q2YCZ3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410770.1
- ProteinModelPortal:   Q2YCZ3
- SMR:   Q2YCZ3
- STRING:   Q2YCZ3
- GeneID:   3785793
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0069
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q2YCZ3
- ProtClustDB:   PRK00121
- BioCyc:   NMUL323848:NMUL_A0069-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26016; Mature: 26016

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 84-84 BINDING 111-111 BINDING 134-134 BINDING 138-138 BINDING 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEHSPHRPIRSFVLRQGRLSNAQRRAHETLMPKYGIPYSGKLLDLAIIFSRSAPKFLEI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEHEEECCCCCCCCEEE
GFGMGETTALIAQAHPQNDYLAAEVHTPGMGSLLKQVEELELTNIRVIQHDAVDVLQHAL
CCCCCCHHEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCC
PPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLKPGGYIHVATDWEDYAEQILE
CHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
VLSGEPHLSNTAVGYAPRPEYRPLTKFEQRGLRLGHEVWDVIFRKKQGA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVEHSPHRPIRSFVLRQGRLSNAQRRAHETLMPKYGIPYSGKLLDLAIIFSRSAPKFLEI
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEHEEECCCCCCCCEEE
GFGMGETTALIAQAHPQNDYLAAEVHTPGMGSLLKQVEELELTNIRVIQHDAVDVLQHAL
CCCCCCHHEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCC
PPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLKPGGYIHVATDWEDYAEQILE
CHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHH
VLSGEPHLSNTAVGYAPRPEYRPLTKFEQRGLRLGHEVWDVIFRKKQGA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA