Click here to switch to the map view.

The map label for this gene is intZ [H]

Identifier: 82701199

GI number: 82701199

Start: 69640

End: 70902

Strand: Reverse

Name: intZ [H]

Synonym: Nmul_A0064

Alternate gene names: 82701199

Gene position: 70902-69640 (Counterclockwise)

Preceding gene: 82701200

Following gene: 82701198

Centisome position: 2.23

GC content: 55.03

Gene sequence:

>1263_bases
ATGGCTAAGCTAACAGACATGGAGATCCGCAACTGGATCAAGGCGGGCGAACGGTTTGAAGGCCGTGCTGTAGGTGGTGG
CCTATATCTGAGTTTTCGTGAAGGTTATGCCATTCCTATCTGGCGTTTCAGATACCGTTTTTGTGGCAAGCGCCGCGTTA
TGAATATTGGCAGTTACGGTACGCTATCCCTGGCGGATGCCAGGGATGAAGCCAAGAAGCTGTCCGCTCGCGTTGCTTTG
GGCTACGACGTGGCCGGAGAAAAACAGCAGCGCAAGGGCGAAGCCATTGCCAGGATAGAAGCGGAGAAGAATGCGTACAC
CGTGGCGCAGCTGGCCGACGAATATTTTGAAAGGATGATTGCAGGGCGATGGAAGCACCCAAACATCGTACGATCCAGGA
TCGAGAAAGACATCAAGCCCGCGATTGGCAGCTTGAAGGTTGAGGATGTAAAGCCCAGGCATATTGATGATGTGCTCAAG
GCTGTAATGAAACGGGGTGCGCCTTCCATAGCGAACGATACACTGCGCTGGCTTAAGCGCATGTTCAACTATGCTATCAA
GCGCCACATCATCGAATACAATCCCGCGGCTGCATTTGATCCAGGTGACGCTGGCGGCAAGGAGAAAAGCCGGACGCGCT
GGTTGACCAGCGAGGAGCTGGTCACGCTCTTTGAAGCAATGCGGCAAGCACCTGGTTTCAGTGTGGAGAACGGCTTGAGC
ATCAAACTGCTATTACTGCTTGCGGTGCGAAAGGGTGAGCTGATCGGCGCCAGGTGGTCTGAGTTCGACCTGGATAAAGC
TGTCTGGTATCTGCCTGCCGAACGCACGAAAACCGAATCTGCCATTGACATACCCTTGCCTCCGATTGCAGTAGAGTGGC
TGCGCGAGTTGCAGCGCCTGGCGGGTGTTAGCAAGTGGGTGTTGCCGGCTCGCAAGATGCAGGATCGGATGATTCCACAT
ATTGCGGAAAGCACGCTGAGCGTGGCTCTGGCAAAGATCAAGCACGGCCTGGAACCCTTCACTATCCATGATCTACGCCG
TACTGCGCGTACGCATTTCGAAGCCCTGGGCGTTGCCCCTCACATTGCCGAGCGTTGCCTGAATCACAAGATCAAGGGGA
TCGAGGGCATCTATAACCGGCACGACTACTTTGAAGAACGCAAGGCGGCACTGGAGGCCTGGGCGGGACTGTTGCTCCAG
ATCGAGCGGGGCGAGGCTGATAAGGTTGTGCCGATCAGGCGTGCCGTAGCAACAAAACAATAA

Upstream 100 bases:

>100_bases
CAGGTTTTTACGTGTACCCAGCGAGAAGCCCGATACAGGCGGGTTTACGTGATATGCCGGGTACCCCTAAAAAAACTTAA
ATCCTGAACAGTGAGTAGAG

Downstream 100 bases:

>100_bases
ACTGTTAAGCCACCCCTATCCAGGCCAGGAGAAAAACGGAAACCTTCACCGCCTGGGGTGGCAACCAATTTGAAGGGATA
CGCTTGGAAGGAAGTGGGAA

Product: Phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAKLTDMEIRNWIKAGERFEGRAVGGGLYLSFREGYAIPIWRFRYRFCGKRRVMNIGSYGTLSLADARDEAKKLSARVAL
GYDVAGEKQQRKGEAIARIEAEKNAYTVAQLADEYFERMIAGRWKHPNIVRSRIEKDIKPAIGSLKVEDVKPRHIDDVLK
AVMKRGAPSIANDTLRWLKRMFNYAIKRHIIEYNPAAAFDPGDAGGKEKSRTRWLTSEELVTLFEAMRQAPGFSVENGLS
IKLLLLLAVRKGELIGARWSEFDLDKAVWYLPAERTKTESAIDIPLPPIAVEWLRELQRLAGVSKWVLPARKMQDRMIPH
IAESTLSVALAKIKHGLEPFTIHDLRRTARTHFEALGVAPHIAERCLNHKIKGIEGIYNRHDYFEERKAALEAWAGLLLQ
IERGEADKVVPIRRAVATKQ

Sequences:

>Translated_420_residues
MAKLTDMEIRNWIKAGERFEGRAVGGGLYLSFREGYAIPIWRFRYRFCGKRRVMNIGSYGTLSLADARDEAKKLSARVAL
GYDVAGEKQQRKGEAIARIEAEKNAYTVAQLADEYFERMIAGRWKHPNIVRSRIEKDIKPAIGSLKVEDVKPRHIDDVLK
AVMKRGAPSIANDTLRWLKRMFNYAIKRHIIEYNPAAAFDPGDAGGKEKSRTRWLTSEELVTLFEAMRQAPGFSVENGLS
IKLLLLLAVRKGELIGARWSEFDLDKAVWYLPAERTKTESAIDIPLPPIAVEWLRELQRLAGVSKWVLPARKMQDRMIPH
IAESTLSVALAKIKHGLEPFTIHDLRRTARTHFEALGVAPHIAERCLNHKIKGIEGIYNRHDYFEERKAALEAWAGLLLQ
IERGEADKVVPIRRAVATKQ
>Mature_419_residues
AKLTDMEIRNWIKAGERFEGRAVGGGLYLSFREGYAIPIWRFRYRFCGKRRVMNIGSYGTLSLADARDEAKKLSARVALG
YDVAGEKQQRKGEAIARIEAEKNAYTVAQLADEYFERMIAGRWKHPNIVRSRIEKDIKPAIGSLKVEDVKPRHIDDVLKA
VMKRGAPSIANDTLRWLKRMFNYAIKRHIIEYNPAAAFDPGDAGGKEKSRTRWLTSEELVTLFEAMRQAPGFSVENGLSI
KLLLLLAVRKGELIGARWSEFDLDKAVWYLPAERTKTESAIDIPLPPIAVEWLRELQRLAGVSKWVLPARKMQDRMIPHI
AESTLSVALAKIKHGLEPFTIHDLRRTARTHFEALGVAPHIAERCLNHKIKGIEGIYNRHDYFEERKAALEAWAGLLLQI
ERGEADKVVPIRRAVATKQ

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=376, Percent_Identity=29.2553191489362, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1788974, Length=395, Percent_Identity=25.8227848101266, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788690, Length=380, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47771; Mature: 47640

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTDMEIRNWIKAGERFEGRAVGGGLYLSFREGYAIPIWRFRYRFCGKRRVMNIGSYG
CCCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHHHCCHHEEECCCCC
TLSLADARDEAKKLSARVALGYDVAGEKQQRKGEAIARIEAEKNAYTVAQLADEYFERMI
CEEEHHHHHHHHHHHHHHEECCCCCCCHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHH
AGRWKHPNIVRSRIEKDIKPAIGSLKVEDVKPRHIDDVLKAVMKRGAPSIANDTLRWLKR
HCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MFNYAIKRHIIEYNPAAAFDPGDAGGKEKSRTRWLTSEELVTLFEAMRQAPGFSVENGLS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCH
IKLLLLLAVRKGELIGARWSEFDLDKAVWYLPAERTKTESAIDIPLPPIAVEWLRELQRL
HHHHHHHHHHCCCEECCCCCCCCCCHHEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHH
AGVSKWVLPARKMQDRMIPHIAESTLSVALAKIKHGLEPFTIHDLRRTARTHFEALGVAP
HCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
HIAERCLNHKIKGIEGIYNRHDYFEERKAALEAWAGLLLQIERGEADKVVPIRRAVATKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AKLTDMEIRNWIKAGERFEGRAVGGGLYLSFREGYAIPIWRFRYRFCGKRRVMNIGSYG
CCCCHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHHHCCHHEEECCCCC
TLSLADARDEAKKLSARVALGYDVAGEKQQRKGEAIARIEAEKNAYTVAQLADEYFERMI
CEEEHHHHHHHHHHHHHHEECCCCCCCHHHHCCHHHHHEECCCCHHHHHHHHHHHHHHHH
AGRWKHPNIVRSRIEKDIKPAIGSLKVEDVKPRHIDDVLKAVMKRGAPSIANDTLRWLKR
HCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
MFNYAIKRHIIEYNPAAAFDPGDAGGKEKSRTRWLTSEELVTLFEAMRQAPGFSVENGLS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCH
IKLLLLLAVRKGELIGARWSEFDLDKAVWYLPAERTKTESAIDIPLPPIAVEWLRELQRL
HHHHHHHHHHCCCEECCCCCCCCCCHHEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHH
AGVSKWVLPARKMQDRMIPHIAESTLSVALAKIKHGLEPFTIHDLRRTARTHFEALGVAP
HCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
HIAERCLNHKIKGIEGIYNRHDYFEERKAALEAWAGLLLQIERGEADKVVPIRRAVATKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]