Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 162139864

GI number: 162139864

Start: 2208567

End: 2209205

Strand: Reverse

Name: leuD [H]

Synonym: Nmul_A1919

Alternate gene names: 162139864

Gene position: 2209205-2208567 (Counterclockwise)

Preceding gene: 82703041

Following gene: 82703039

Centisome position: 69.38

GC content: 54.46

Gene sequence:

>639_bases
GTGAAAAAGTTTCATTCATGCGAGGGAGTGGTGGTCCCACTGGATCGGGCGAACGTCGACACCGACGCCATCATTCCCAA
ACAGTTTCTGAAATCCATCAAGAGGTCCGGCTTCGGGCAAAACCTCTTCGATGAATGGCGATACCTGGATCATGGCGAGC
CAGGCATCGACCCTGCCACTCGTAAACTCAACCCTGAATTTGTGCTTAACCTGCCGCGTTACCGCGATGCCCGGCTACTC
CTCGCCCGCGCCAATTTCGGTTGCGGATCCAGCCGGGAACATGCTCCCTGGGCATTGCAGGATTACGGCTTTGAGGTAAT
CATTGCCCCCAGTTTTGCCGATATTTTTTTCAACAACTGCTTCAAAATAGGTTTGCTTCCCATCGTGCTCGACACCTCAC
AGGTGGATCAGTTGTTCCGCGAGGTCGAGGCCATCGAAGGGTACCGATTGCTCGTCAATCTGGAGCAGCAATCGGTGACC
ACTCCTGCCGGAGAATCTTTCTCCTTCGATATCGATCCTTTCCGCAAGCATAGTCTGCTGAATGGTCTGGACGAAATCGG
CCTCACGTTGCAGCACGCGGACAAAATCCGCGCGTTCGAAGAAAAACGCCGCGCAGAACAGCCGTGGCTGTTCGCCTGA

Upstream 100 bases:

>100_bases
TGTCTGCCTGCCACACCATTCAGGGAGTAGGCAAAGACGTTCAAAGCGGCGGAGAAGCACTGGAAAAATCTGCAAAATAA
TTGCAAAACGGTAATCAGCA

Downstream 100 bases:

>100_bases
CAGACTTTCCCCTTTAAAAGGACCTTATGAAAATAGCAATTCTGGCCGGAGATGGTATCGGCCCGGAAATTGTCGCGCAA
GCGGTGCGCGTGCTGGAAAC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKFHSCEGVVVPLDRANVDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDHGEPGIDPATRKLNPEFVLNLPRYRDARLL
LARANFGCGSSREHAPWALQDYGFEVIIAPSFADIFFNNCFKIGLLPIVLDTSQVDQLFREVEAIEGYRLLVNLEQQSVT
TPAGESFSFDIDPFRKHSLLNGLDEIGLTLQHADKIRAFEEKRRAEQPWLFA

Sequences:

>Translated_212_residues
MKKFHSCEGVVVPLDRANVDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDHGEPGIDPATRKLNPEFVLNLPRYRDARLL
LARANFGCGSSREHAPWALQDYGFEVIIAPSFADIFFNNCFKIGLLPIVLDTSQVDQLFREVEAIEGYRLLVNLEQQSVT
TPAGESFSFDIDPFRKHSLLNGLDEIGLTLQHADKIRAFEEKRRAEQPWLFA
>Mature_212_residues
MKKFHSCEGVVVPLDRANVDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDHGEPGIDPATRKLNPEFVLNLPRYRDARLL
LARANFGCGSSREHAPWALQDYGFEVIIAPSFADIFFNNCFKIGLLPIVLDTSQVDQLFREVEAIEGYRLLVNLEQQSVT
TPAGESFSFDIDPFRKHSLLNGLDEIGLTLQHADKIRAFEEKRRAEQPWLFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=205, Percent_Identity=59.0243902439024, Blast_Score=235, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321429, Length=213, Percent_Identity=49.2957746478873, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24212; Mature: 24212

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFHSCEGVVVPLDRANVDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDHGEPGIDPAT
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHH
RKLNPEFVLNLPRYRDARLLLARANFGCGSSREHAPWALQDYGFEVIIAPSFADIFFNNC
HCCCHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCEEEEECCCHHHHHHHHH
FKIGLLPIVLDTSQVDQLFREVEAIEGYRLLVNLEQQSVTTPAGESFSFDIDPFRKHSLL
HHHCCEEEEECCHHHHHHHHHHHHHCCEEEEEEECHHCCCCCCCCCEEECCCHHHHHHHH
NGLDEIGLTLQHADKIRAFEEKRRAEQPWLFA
HHHHHHCEEHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKFHSCEGVVVPLDRANVDTDAIIPKQFLKSIKRSGFGQNLFDEWRYLDHGEPGIDPAT
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHH
RKLNPEFVLNLPRYRDARLLLARANFGCGSSREHAPWALQDYGFEVIIAPSFADIFFNNC
HCCCHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCEEEEECCCHHHHHHHHH
FKIGLLPIVLDTSQVDQLFREVEAIEGYRLLVNLEQQSVTTPAGESFSFDIDPFRKHSLL
HHHCCEEEEECCHHHHHHHHHHHHHCCEEEEEEECHHCCCCCCCCCEEECCCHHHHHHHH
NGLDEIGLTLQHADKIRAFEEKRRAEQPWLFA
HHHHHHCEEHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA