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The map label for this gene is leuB

Identifier: 82703039

GI number: 82703039

Start: 2207479

End: 2208540

Strand: Reverse

Name: leuB

Synonym: Nmul_A1918

Alternate gene names: 82703039

Gene position: 2208540-2207479 (Counterclockwise)

Preceding gene: 162139864

Following gene: 82703038

Centisome position: 69.36

GC content: 57.91

Gene sequence:

>1062_bases
ATGAAAATAGCAATTCTGGCCGGAGATGGTATCGGCCCGGAAATTGTCGCGCAAGCGGTGCGCGTGCTGGAAACGCTGAG
AAGCGATGGATTGAAGCTGGAACTGGAACAGGGATTGCTGGGCGGATGTGCTGTAGATGCAGCGGGCGAGCCTTTTCCCG
CGGCAACGCGCACATTGGTGGCTCAGGCGGACGCCGTGATCCTGGGGGCAGTGGGCGGCCCGCGATATGACGGGCTGCCC
CGGCAGCTCAGGCCGGAGCAAGGCCTTCTTGGCATACGGAAGGCCTTGAACCTGTTTGCCAATCTCCGGCCTGCGGTACT
TTATCCTGAACTTGCCGATGCCTCCACGCTGAAGCCCGAGGTGGTGTCCGGACTCGATATCCTGATCGTGCGCGAGTTGA
CCGGGGATATTTACTTTGGTGAGCCACGCGGGATTGAATTACGGAATGGTCAGCGCATCGGCTACAATACCATGATTTAC
AGCGAAGCCGAGATCCGGAGAATAGCGCGGGTGGCTTTCCAGGCAGCGCGCAAGCGCAGTCGCAGGCTGTGCTCTGTCGA
CAAGATGAACGTACTGGAATCAACCCAGCTGTGGCGCGACGTGGTGACCGAAACGGCAGGTGAATATCCGGACGTGGAGC
TTTCGCACATGCTGGTGGACAATGCGGCCATGCAGCTTGTACGCAATCCCCGGCAGTTCGATGTGGTTGTGACAGGCAAT
ATGTTCGGGGACATCCTGTCGGATGAAGCATCCATGTTGACCGGTTCGATCGGCATGCTGCCTTCGGCATCGCTCGATGA
GCGGAACAAGGGGCTTTATGAACCCATACACGGTTCTGCTCCCGATATCGCCGGCAAGGACGTGGCGAATCCTCTGGCCA
CCGTCCTTTCAGTTGCGATGATGCTGCGCTATACCTTCGATCGGGAGGAGGAGGCATCCCGAATCGAACGGGCAGTGAAA
AAGGTGCTGGCTGATGGATACCGGACGGCGGATATTTACGAGCCAGGAAAGATGAAAATCGGAACCGCAGCAATGGGTGA
TGCGGTTCTGGCAAGTTTGTAG

Upstream 100 bases:

>100_bases
CGTTGCAGCACGCGGACAAAATCCGCGCGTTCGAAGAAAAACGCCGCGCAGAACAGCCGTGGCTGTTCGCCTGACAGACT
TTCCCCTTTAAAAGGACCTT

Downstream 100 bases:

>100_bases
CAACGACACTTCATATCCTGTGGTTGATCAACCACGATTTCTTCCAAAAGAGAGATATCAATGAAACGAGTGGGTTTTAT
CGGTTGGCGTGGCATGGTAG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKIAILAGDGIGPEIVAQAVRVLETLRSDGLKLELEQGLLGGCAVDAAGEPFPAATRTLVAQADAVILGAVGGPRYDGLP
RQLRPEQGLLGIRKALNLFANLRPAVLYPELADASTLKPEVVSGLDILIVRELTGDIYFGEPRGIELRNGQRIGYNTMIY
SEAEIRRIARVAFQAARKRSRRLCSVDKMNVLESTQLWRDVVTETAGEYPDVELSHMLVDNAAMQLVRNPRQFDVVVTGN
MFGDILSDEASMLTGSIGMLPSASLDERNKGLYEPIHGSAPDIAGKDVANPLATVLSVAMMLRYTFDREEEASRIERAVK
KVLADGYRTADIYEPGKMKIGTAAMGDAVLASL

Sequences:

>Translated_353_residues
MKIAILAGDGIGPEIVAQAVRVLETLRSDGLKLELEQGLLGGCAVDAAGEPFPAATRTLVAQADAVILGAVGGPRYDGLP
RQLRPEQGLLGIRKALNLFANLRPAVLYPELADASTLKPEVVSGLDILIVRELTGDIYFGEPRGIELRNGQRIGYNTMIY
SEAEIRRIARVAFQAARKRSRRLCSVDKMNVLESTQLWRDVVTETAGEYPDVELSHMLVDNAAMQLVRNPRQFDVVVTGN
MFGDILSDEASMLTGSIGMLPSASLDERNKGLYEPIHGSAPDIAGKDVANPLATVLSVAMMLRYTFDREEEASRIERAVK
KVLADGYRTADIYEPGKMKIGTAAMGDAVLASL
>Mature_353_residues
MKIAILAGDGIGPEIVAQAVRVLETLRSDGLKLELEQGLLGGCAVDAAGEPFPAATRTLVAQADAVILGAVGGPRYDGLP
RQLRPEQGLLGIRKALNLFANLRPAVLYPELADASTLKPEVVSGLDILIVRELTGDIYFGEPRGIELRNGQRIGYNTMIY
SEAEIRRIARVAFQAARKRSRRLCSVDKMNVLESTQLWRDVVTETAGEYPDVELSHMLVDNAAMQLVRNPRQFDVVVTGN
MFGDILSDEASMLTGSIGMLPSASLDERNKGLYEPIHGSAPDIAGKDVANPLATVLSVAMMLRYTFDREEEASRIERAVK
KVLADGYRTADIYEPGKMKIGTAAMGDAVLASL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=32.1212121212121, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178816, Length=319, Percent_Identity=28.5266457680251, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=319, Percent_Identity=28.5266457680251, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4758582, Length=319, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI28178838, Length=319, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI28178819, Length=162, Percent_Identity=33.9506172839506, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=351, Percent_Identity=51.2820512820513, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=33.8888888888889, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787381, Length=351, Percent_Identity=25.9259259259259, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=350, Percent_Identity=32.2857142857143, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=30.1492537313433, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=335, Percent_Identity=29.8507462686567, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=337, Percent_Identity=28.7833827893175, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=45.2777777777778, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=34.4827586206897, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=33.4310850439883, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24643268, Length=337, Percent_Identity=33.2344213649852, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24643270, Length=337, Percent_Identity=33.2344213649852, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24661184, Length=367, Percent_Identity=31.6076294277929, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078635, Length=247, Percent_Identity=29.1497975708502, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078637, Length=247, Percent_Identity=29.1497975708502, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078639, Length=247, Percent_Identity=29.1497975708502, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078633, Length=247, Percent_Identity=29.1497975708502, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24650122, Length=247, Percent_Identity=29.1497975708502, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281362242, Length=336, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648872, Length=336, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130355, Length=222, Percent_Identity=27.4774774774775, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_NITMU (Q2Y7Q8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412605.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2Y7Q8
- SMR:   Q2Y7Q8
- STRING:   Q2Y7Q8
- GeneID:   3784156
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1918
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- PhylomeDB:   Q2Y7Q8
- ProtClustDB:   PRK00772
- BioCyc:   NMUL323848:NMUL_A1918-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38195; Mature: 38195

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 93-93 BINDING 103-103 BINDING 131-131 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAILAGDGIGPEIVAQAVRVLETLRSDGLKLELEQGLLGGCAVDAAGEPFPAATRTLV
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHH
AQADAVILGAVGGPRYDGLPRQLRPEQGLLGIRKALNLFANLRPAVLYPELADASTLKPE
HHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHH
VVSGLDILIVRELTGDIYFGEPRGIELRNGQRIGYNTMIYSEAEIRRIARVAFQAARKRS
HHCCCCEEEEEECCCCEEECCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
RRLCSVDKMNVLESTQLWRDVVTETAGEYPDVELSHMLVDNAAMQLVRNPRQFDVVVTGN
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
MFGDILSDEASMLTGSIGMLPSASLDERNKGLYEPIHGSAPDIAGKDVANPLATVLSVAM
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MLRYTFDREEEASRIERAVKKVLADGYRTADIYEPGKMKIGTAAMGDAVLASL
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHCC
>Mature Secondary Structure
MKIAILAGDGIGPEIVAQAVRVLETLRSDGLKLELEQGLLGGCAVDAAGEPFPAATRTLV
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHH
AQADAVILGAVGGPRYDGLPRQLRPEQGLLGIRKALNLFANLRPAVLYPELADASTLKPE
HHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHH
VVSGLDILIVRELTGDIYFGEPRGIELRNGQRIGYNTMIYSEAEIRRIARVAFQAARKRS
HHCCCCEEEEEECCCCEEECCCCCEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
RRLCSVDKMNVLESTQLWRDVVTETAGEYPDVELSHMLVDNAAMQLVRNPRQFDVVVTGN
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
MFGDILSDEASMLTGSIGMLPSASLDERNKGLYEPIHGSAPDIAGKDVANPLATVLSVAM
HHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MLRYTFDREEEASRIERAVKKVLADGYRTADIYEPGKMKIGTAAMGDAVLASL
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA