Click here to switch to the map view.

The map label for this gene is xylF [H]

Identifier: 82545937

GI number: 82545937

Start: 3580253

End: 3581224

Strand: Direct

Name: xylF [H]

Synonym: SBO_3574

Alternate gene names: 82545937

Gene position: 3580253-3581224 (Clockwise)

Preceding gene: 82545932

Following gene: 82545938

Centisome position: 79.21

GC content: 44.03

Gene sequence:

>972_bases
ATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTTCTGCTTACCAACGTTGCGGCACACGCCAAAGAAGTCAA
AATAGGTATGGCGATTGATGATCTACGTCTTGAACGCTGGCAAAAAGATCGAGATATTTTTGTGAAAAAGGCTGAATCTC
TCGGTGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGGAGAAACACAAATGTCGCAGATTGAAAACATGATTAACCGG
GGTGTCGATGTTCTTGTCATTATTCCGTATAACGGCCAGGTATTAAGTAACGTTGTAAAAGAAGCCAAACAAGAAGGTAT
TAAAGTATTAGCTTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTG
AACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAAC
GCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCA
ATGGGTTGATGGCTGGTTACCGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATG
CCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATTTCCGGCCAGGATGCGGAT
CTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACATTGCTGGCAAATACTGC
CGCAGAAATTGCCGTTGAATTGGGCAATGGTCAGGAGCCAAAAGCAGATACCTCACTGAATAATGGCCTGAAAGATGTCC
CCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAG
AGCGAGCTGTAA

Upstream 100 bases:

>100_bases
GTAATTGTTTTCCCCTGTTTAGTTGCTAAAAATTGGTTACGTTTATCGCGGTGATTGTTACTTATTAAAGCTGTCCTCTA
ACTACAGAAGGCCCTACACC

Downstream 100 bases:

>100_bases
GCGTTACGCCCCGGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATG
CCTTATCTACTTGAAATGAA

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDAD
LAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKE
SEL

Sequences:

>Translated_323_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDAD
LAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKE
SEL
>Mature_323_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDAD
LAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKE
SEL

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=330, Percent_Identity=97.2727272727273, Blast_Score=613, Evalue=1e-177,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34848; Mature: 34848

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
QSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDAD
EECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGK
EEEEEEECCCCHHHHHHEEHEEEECCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCC
IKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDAD
EEEEECCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCC
LAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTP
HHHHHHHHCCCCEEEEECCHHHHHCCHHHEEEECCCCCCCCCCCCHHCCHHHHHHHHCCC
IDVNKNNIKDTVIKDGFHKESEL
CCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
QSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDAD
EECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGK
EEEEEEECCCCHHHHHHEEHEEEECCCCEEEECCCCCCCCCEEEECCCEEEEEECCCCCC
IKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDAD
EEEEECCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCCCCC
LAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTP
HHHHHHHHCCCCEEEEECCHHHHHCCHHHEEEECCCCCCCCCCCCHHCCHHHHHHHHCCC
IDVNKNNIKDTVIKDGFHKESEL
CCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]