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The map label for this gene is nrdF [H]

Identifier: 82545243

GI number: 82545243

Start: 2849115

End: 2850029

Strand: Reverse

Name: nrdF [H]

Synonym: SBO_2841

Alternate gene names: 82545243

Gene position: 2850029-2849115 (Counterclockwise)

Preceding gene: 82545244

Following gene: 82545241

Centisome position: 63.06

GC content: 47.98

Gene sequence:

>915_bases
ATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAATCGCCTGAC
CAGCAATTTCTGGCTACCAGAAAAGGTGCCGCTGTCGAACGATATTCCTGCCTGGCAGACATTAACTGTCGTAGAACAAC
AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCGCTGATGCCC
TATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTC
GATTTTCTCGACGCTGTGTCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAA
AAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTT
TTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCAGATCTGATCCGTCT
GATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGAC
AACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACAGCGATGAGCTG
TACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTA
CGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATC
ACGATTTCTTTTCCGGTTCACGTATAGGAAATTGA

Upstream 100 bases:

>100_bases
CAAGGGTATCAAAACGCTCTATTACATTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCT
GTGCATTTTAAGGAATATCT

Downstream 100 bases:

>100_bases
AAAACTAAGCGATACCCCTGTCCGCGCCTTATACCACGTGAGCTTTTCTGCTTTTCCCTCCACTCCGGTGTTTTCTCTCT
CTGCATGGTCGATTCTTATA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
YALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYSDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSRIGN

Sequences:

>Translated_304_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
YALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYSDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSRIGN
>Mature_304_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
YALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYSDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSRIGN

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=300, Percent_Identity=99.3333333333333, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1788567, Length=265, Percent_Identity=24.9056603773585, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 34772; Mature: 34772

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYSDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HCCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
RIGN
CCCC
>Mature Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLTVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREELKSFAFDLLLELYDNELQYSDEL
HHHHHHHHHHHCCCCEEEEEEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCCCHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HCCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
RIGN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2649479; 8195103 [H]