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The map label for this gene is nrdE [H]

Identifier: 82545244

GI number: 82545244

Start: 2850039

End: 2852183

Strand: Reverse

Name: nrdE [H]

Synonym: SBO_2842

Alternate gene names: 82545244

Gene position: 2852183-2850039 (Counterclockwise)

Preceding gene: 82545245

Following gene: 82545243

Centisome position: 63.1

GC content: 52.35

Gene sequence:

>2145_bases
TTGGCAACGACAACCGCAGAATGCCTGACGCAGGAAACGATGGATTACCACGCGCTGAATGCGATGCTTAACCTCTACGA
TAGCGCAGGTCGCATTCAGTTCGATAAAGACCGCCAGGCCGTTGACGCCTTTATGGCGACGCATGTGCGTCCGAACAGTG
TGACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTAC
TCTCGCGACTTTGTCATTACGCTGTTTGCCTACGCACACACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAA
GTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAACGTTATCTGGAAGATTTTGCCGATCGAGTCACGATGGTGG
CGCTGACGCTGGCACAAGGCGATGCGACGCTGGCGTTGCAACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCC
ACTCCGACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTCTGCTGCGTATTGAAGACAATAT
GGAGTCGATTGGCCGGGCGGTAAATTCCGCACTGCAACTGTCGAAACGCGGCGGCGGCGTGGCATTTTTGCTGTCGAATC
TGCGAGAAGCGGGCGCGCCAATTAAACGTATTGAAAATCAATCTTCTGGCGTAATTCCGGTGATGAAAATGCTGGAAGAC
GCATTTTCCTATGCCAACCAACTCGGCGCGCGTCAGGGGGCAGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCT
GCGTTTTCTCGATACAAAACGGGAAAATGCCGACGAAAAAATCCGCATTAAAACACTGTCGCTGGGCGTGGTGATCCCGG
ATATCACTTTCCATCTGGCAAAAGAGAATGCGCAGATGGCGCTGTTTTCGCCCTATGACGTTGAGCGAGTTTATGGCAAG
CCGTTTGCCGATATCGCCATCAGCGAACACTATGACGAACTGGTTGCCGATGAACGCATCCGCAAAAAATACCTCAACGC
CCGTGATTTCTTCCAGCGGCTGGCAGAAATCCAGTTTGAGTCCGGCTATCCCTACATCATGTATGAAGACACGGTAAACC
GTGCTAACCCTGTCGCCGGGCGCATAAATATGAGTAATCTCTGCTCAGAGATTTTGCAGGTCAACTGTGCCTCAGAGTAT
GACGAGAATCTCGACTATGCCCGCACAGGCCATGATATCTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGA
TTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCGGTGTCAGATATGAGTCATATCCGCAGCG
TGCCGTCCATCGAAGCCGGAAATGCCGCCTCACACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGA
GAAGGCATCGCTTATGGTTCGCCGGAAGCACTGGATTTCACCAATCTCTATTTCTATGCCATCACCTGGCACGCACTGCG
TACCTCGATGTTGCTGGCGCGCGAACGCGGTGAAACCTTCGCCGGGTTCAAACAGTCACGTTATGCCAGTGGTGAATATT
TTAGCCAATATCTGCAAGGTAACTGGCAGCCGAAAACGGCGAAAGTTGGCGAACTGTTTGCCCGCAGCGGTATTACGTTA
CCTACCCGTGAGATGTGGGCGCAACTGCGCGACGACGTGATGCGCTACGGCATTTATAACCAGAATCTTCAGGCGGTGCC
GCCAACCGGTTCTATCTCTTATATCAACCATGCTACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAG
AGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAGAATCTGGCGCTGTATCAGGATGCTTACGAA
ATCGGCGCAGAAAAGATCATCGACACCTACGCCGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTGTTTTT
CCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAGGGTATCAAAACGCTCTATTACA
TTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCTGTGCATTTTAA

Upstream 100 bases:

>100_bases
ATGTGATTGCCCGGAAATGCGGCGTGCCGTGGCTGTACCGTTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTT
CGTAAAGGAGTAACCGAATT

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
TCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAYAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAF

Sequences:

>Translated_714_residues
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAYAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAF
>Mature_713_residues
ATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYS
RDFVITLFAYAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPAT
PTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDA
FSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKP
FADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYD
ENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLP
TREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEI
GAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=669, Percent_Identity=27.2047832585949, Blast_Score=183, Evalue=5e-46,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=98.4593837535014, Blast_Score=1476, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.9450171821306, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=748, Percent_Identity=25, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=620, Percent_Identity=26.1290322580645, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=25.8164852255054, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17352481, Length=616, Percent_Identity=24.025974025974, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80427; Mature: 80296

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHEEHHEEEECCCEEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAF
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure 
ATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMATHVRPNSVTFSSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHEEHHEEEECCCEEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPVAGRINMSNLCSEILQVNCASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
CCCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAF
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]