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The map label for this gene is hyfG [H]

Identifier: 82544936

GI number: 82544936

Start: 2502101

End: 2503816

Strand: Direct

Name: hyfG [H]

Synonym: SBO_2508

Alternate gene names: 82544936

Gene position: 2502101-2503816 (Clockwise)

Preceding gene: 82544935

Following gene: 82544937

Centisome position: 55.36

GC content: 54.43

Gene sequence:

>1716_bases
GTGAACGTTAATTCATCGTCAAATAGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTACTGGA
TGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACATTATCTTTATT
ATCAACATGATGGCTGGCTTCCAGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCC
CTTTCAATGGAAGGGGCCGAAAAATGCTGGATCGTGGTGAAGGCACTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGT
CACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGCGATATGTACGGGCTGATTCCGGTTGGCCTGCCGG
ATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCACAAAGATGCGATGGATTATCGACTG
CGCCCTGAACCGACGACAGATTCCGAAACGTATGCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGG
CCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACC
GCCTGTTTTATGTCCATCGAGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGAC
CGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAACTCGGTTGAAAATGCACTGGGGATTGAGGTGCC
GCAACGAGCGCATACCATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTCAACCTTGGCCTCTCCT
GCCATTTTGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTG
ACCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAAC
GCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAAC
GCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTATAGCCCTGTAGGGCCGCTGATCCGCGGCAGTGGT
TTTGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTCACCGGCGG
CGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGGAATTTGCCCTCGACAACA
TGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGC
GGTGAAGACGTGCACTGGAGCATGCTCGGTGATAACCAAAAATTGTTCCGCTGGCGCTGCCGTGCCGCCACCTACGCCAA
CTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCT
ACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGC
TACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAA

Upstream 100 bases:

>100_bases
TCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGC
ATCTGTTTCGGAGAAACACA

Downstream 100 bases:

>100_bases
GGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGA
GGTCAGCCCAGGCTTTCGCG

Product: hydrogenase 4 subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK

Sequences:

>Translated_571_residues
MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK
>Mature_571_residues
MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=383, Percent_Identity=27.154046997389, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI260898743, Length=373, Percent_Identity=27.0777479892761, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1788832, Length=571, Percent_Identity=96.3222416812609, Blast_Score=1129, Evalue=0.0,
Organism=Escherichia coli, GI1789076, Length=568, Percent_Identity=72.1830985915493, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=493, Percent_Identity=27.5862068965517, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555284, Length=392, Percent_Identity=26.0204081632653, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568379, Length=392, Percent_Identity=25.5102040816327, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI221459469, Length=386, Percent_Identity=26.6839378238342, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24638644, Length=380, Percent_Identity=27.8947368421053, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 64941; Mature: 64941

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHEECCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRLRPEPTTDSETYAFINEGNSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHHCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVHYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECHHHHHHHHHEECCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRLRPEPTTDSETYAFINEGNSD
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHHCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]