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The map label for this gene is hyfF [H]

Identifier: 82544935

GI number: 82544935

Start: 2500531

End: 2502111

Strand: Direct

Name: hyfF [H]

Synonym: SBO_2507

Alternate gene names: 82544935

Gene position: 2500531-2502111 (Clockwise)

Preceding gene: 82544934

Following gene: 82544936

Centisome position: 55.32

GC content: 54.02

Gene sequence:

>1581_bases
ATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACG
GGGGCTCTCTGCGACTTGTACGGTGACTGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGG
TCCAAACTGCCGCTGATGCAGGAGAAATATTCGCTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTC
GCCATTCTTGGTGTGATTGGCTTTCTCACCGGTGTTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCT
TTCACCCGTTACACTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATA
ACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCA
TCGCTGGAATCAGCATGGAAGTACATCATTATTTGTACTGTTGATGTCGCTTTTGGTCTGTTCGGTACCGTGCTGGTATA
CGCCAACGCCGCCAGCGTTATGCCGCAGGCAGAAATGGCGATATTCTGGAGCGAGGTTCTTAAGCAATCGTCCTTGCTTG
ACCCAACATTAATGCTGTTGGCCTTTGTGTTTGTGCTAATTGGCTTTGGTACCAAAACCGGGCTATTTCCCATGCACGCC
TGGCTGCCGGATGCTCACAGTGAAGCGCCGAGTCCGGTCAGCGCCCTGCTCTCCGCCGTATTGCTGAACTGCGCGCTGTT
GGTGCTGATTCGCTATTACATCATTATTTGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCA
TGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAG
AACATGGGGCTGGTCGCGGTGGCGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACACCTTAAACCA
CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCT
GTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTC
AACATTTTTCTTAGCGAATTTATGACCGTTACCGCAGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATT
GCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACC
GGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCA
CAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCAC
CTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
ATCTTCGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGAC
CGCGCTGAAGGGATAATGAG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATAGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTACTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_526_residues
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFL
AILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVE
NMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPF
NIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_525_residues
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLA
ILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAW
LPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVEN
MGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFN
IFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQ
PVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=416, Percent_Identity=26.6826923076923, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI251831116, Length=185, Percent_Identity=31.3513513513514, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=526, Percent_Identity=98.2889733840304, Blast_Score=1025, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=29.9719887955182, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=29.3302540415704, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=27.5462962962963, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788614, Length=439, Percent_Identity=27.5626423690205, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI2367154, Length=194, Percent_Identity=36.0824742268041, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=32.6829268292683, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56578; Mature: 56447

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYIIICTVDVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCHHHHHHHCCEEEEEEHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGITLLLILALWVVQTAADA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
GEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVAHGELSPVTLCDYYGFFH
HHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLESAWKYIIICTVDVAFGL
HHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCHHHHHHHCCEEEEEEHHHHHHH
FGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHH
WLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
VLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLL
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHH
IIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
QPVIRILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]