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The map label for this gene is hyaD [H]

Identifier: 82544702

GI number: 82544702

Start: 2243958

End: 2244545

Strand: Reverse

Name: hyaD [H]

Synonym: SBO_2256

Alternate gene names: 82544702

Gene position: 2244545-2243958 (Counterclockwise)

Preceding gene: 82544703

Following gene: 82544701

Centisome position: 49.66

GC content: 58.67

Gene sequence:

>588_bases
ATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGA
ACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTGGATGGCGGTACTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCC
GGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGCTGGCGCTGGC
AGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGGCTGCAACCCGCGATGCTCGACGACTACGGCGGCAGCC
TGAGCGAACTGGCACGGGAGCAACTGCTCGCTGCGGAACAATCGGCGCTGGCGCAGTTGGCTGCGTGGGGAAGTGTGCCG
CAACTGGCTAATGAATCGCGTTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTA
CCGGATGACACAGGAGGAGCAGGGATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGGCACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAA
ATAAGTAACAAGGAGTGTTC

Downstream 100 bases:

>100_bases
GCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCGGTCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVPQ
LANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=195, Percent_Identity=97.9487179487179, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1789367, Length=148, Percent_Identity=40.5405405405405, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21569; Mature: 21438

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVPQLANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCC
GVRLRQYRMTQEEQG
CCEEEEECCCCCCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGTQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVPQLANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCC
GVRLRQYRMTQEEQG
CCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]