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The map label for this gene is focA [H]

Identifier: 82544642

GI number: 82544642

Start: 2168117

End: 2168974

Strand: Reverse

Name: focA [H]

Synonym: SBO_2190

Alternate gene names: 82544642

Gene position: 2168974-2168117 (Counterclockwise)

Preceding gene: 82544643

Following gene: 82544641

Centisome position: 47.99

GC content: 48.95

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCTTTTGATCTTTTACTTCCTGCTGCAATGGCCAAAGTGGCCGAAGAGGCGGGTGTCTATAAAGC
AACGAAACATCCGCTTAAGACTTTCTATCTGGCGATTACCGCCGGTGTTTTCATCTCAATCGCATTCGTCTTCTATATCA
CAGCAACCACTGGCACAGGCACAATGCCCTTCGGCATGGCAAAACTGGTTGGCGGCATTTGCTTCTCTCTGGGGCTGATT
CTTTGTGTTGTCTGCGGAGCTGATCTCTTTACTTCCACCGTGTTGATTGTTGTTGCTAAGGCGAGTGGGCGCATCACCTG
GGGTCAGTTGGCGAAAAACTGGCTAAATGTCTATTTTGGCAACCTGGTCGGCGCACTGCTGTTTGTACTTTTAATGTGGC
TTTCCGGCGAGTATATGACCGCAAATGGTCAATGGGGACTAAACGTCCTACAAACCGCCGACCACAAAGTGCACCATACT
TTTATTGAGGCCGTCTGTCTTGGTATCCTGGCAAACCTGATGGTATGTCTGGCAGTATGGATGAGTTATTCTGGCCGCAG
CCTGATGGACAAAGCGTTCATTATGGTGCTGCCGGTCGCGATGTTTGTGCCCAGCGGTTTTGAGCACAGTATCGCAAACA
TGTTTATGATCCCGATGGGTATTGTAATCCGCGACTTCGCATCTCCGGAATTCTGGACCGCTGTCGGTTCTGCACCGGAA
AATTTTTCTCACCTGACCGTGATGAACTTCATCACTGATAACCTGATCCCGGTTACGATCGGTAATATTATCGGCGGTGG
TTTGTTGGTTGGGTTGACATACTGGGTCATTTACCTGCGTGAAAACGATCACCATTAA

Upstream 100 bases:

>100_bases
TTACGGACCTATAAGCCAGGCGAGATATGATCTATATCAATTTCTCATCTATAATGCTTTGTTAGTATCTCGTCGCCGAC
TTAATAAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
TGGTTGTCGAAGTACGCAGTAAATAAAAAATCCACTTAAGAAGGTAGGTGTTACATGTCCGAGCTTAATGAAAAGTTAGC
CACAGCCTGGGAAGGTTTTA

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVPSGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Sequences:

>Translated_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVPSGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
>Mature_285_residues
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLI
LCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVPSGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=99.6491228070175, Blast_Score=576, Evalue=1e-166,
Organism=Escherichia coli, GI1788837, Length=282, Percent_Identity=50, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI87082249, Length=268, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=29.4685990338164, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31017; Mature: 31017

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVPSGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHH
NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKAFIMVLPVAMFVPSGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
HCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]