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The map label for this gene is intE [H]

Identifier: 82544409

GI number: 82544409

Start: 1910919

End: 1911962

Strand: Direct

Name: intE [H]

Synonym: SBO_1938

Alternate gene names: 82544409

Gene position: 1910919-1911962 (Clockwise)

Preceding gene: 82544408

Following gene: 82544410

Centisome position: 42.28

GC content: 45.4

Gene sequence:

>1044_bases
ATGTCACCGAGGCCACGAAAAAACAGCACTGACGTAGCCGGTCTTTACGAAAAGTTTGATCGCAGAACTGGCAGAGTTTA
CTACCAGTATAAAAATCCTGTGACTGGAAAATTTCACGGACTCGGAACAGACAAAGGTAAGGCAGAAAAAATCGCTTCCA
CAGCCAATCAGCGAATAGCTGCAGCAGAAGCTGAATATTTCATGCGCAAAATTGATGAAAGTCCGTCAGCAACAAAACGT
CGGGGTATCAGATTAAAGGCATGGGTTGATCGATATCTGAAAATACAGGACACGCGACTGAAAAATGGAGATATTGCAGC
TACAACTCACAAAGAAAAAACTCGAATGGCTGCATACCTGGTTTCCCGTCTGGGAAACCACCCATTGAAAGAACTGGAAG
TAAGAGACTTTGCATTAATACTGGATGAGTGGCTGGATAAAGACATGGTCAGCACAGCGAGAGTAAATCGTGGATTATGG
GTTGATATTTATAAAGAAGCACAGCATGCAGGGGAAGTTCCTCCTGGATGGAATCCTCCGGAGGCTACCCGTAAACCGAT
CCCTAAAGTAACCAGAGCCAGGCTCACCATGGAAGACTGGCAAAAAATTTACAATGCAACGCCTGAAAAACACTTTATCC
GTAACGCAATGCTTCTTGCGATTGTTACTGGTCAGCGCCGTGATGACATTTGCCACATGCGTTTTTCAGATGTGTGGAAC
GAACACTTGCATATCACCCAGGGAAAAACCGGAATGCGTCTGGCGTTACCGCTTACACTACGCTGTGATGCCATTGGGAT
AACGTTAAAAGAAGTTATTGATGGGTGCCGAGACAGAATATTAAGTCCATATCTAATCCATAGTCGGCACCAGAAACAAC
CGAAGCCGATGAGTAAAGACAACCTGAGCGACTACTTTGCCAAAGCACGGGATCTGGCTGGGATATTCCACCAGCAGGAA
AAACTCCGCCAACATTTCATGAACAACGCTCTCTATCAGAACGGCTGTACCGTGCACAGGGTATCGATACAAAAACATTA
CTAG

Upstream 100 bases:

>100_bases
CTGATAGATCGCCGAGCAGTATTTGTTGGGCCAGGTGAAACGGGAATTGCGCCGGAAATTCATACTGGCGATGATGATGC
ACTGAAGGAGATTTTAACTC

Downstream 100 bases:

>100_bases
GACATAAAGTCCAGGCAACCACCGATCGCTATAACGATACTCGAGGTCAGGAATGGGTTTAAGCTGGTTATTTGACAAAC
ACCATATGGAATATCCGTCA

Product: integrase

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIFHQQE
KLRQHFMNNALYQNGCTVHRVSIQKHY

Sequences:

>Translated_347_residues
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIFHQQE
KLRQHFMNNALYQNGCTVHRVSIQKHY
>Mature_346_residues
SPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRR
GIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWV
DIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWNE
HLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIFHQQEK
LRQHFMNNALYQNGCTVHRVSIQKHY

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=340, Percent_Identity=43.5294117647059, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 40145; Mature: 40013

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIA
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLAGIFHQQEKLRQHFMNNALYQNGCTVHRVSIQKHY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure 
SPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLAGIFHQQEKLRQHFMNNALYQNGCTVHRVSIQKHY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]