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The map label for this gene is ldhA [H]

Identifier: 82544172

GI number: 82544172

Start: 1670039

End: 1671028

Strand: Direct

Name: ldhA [H]

Synonym: SBO_1686

Alternate gene names: 82544172

Gene position: 1670039-1671028 (Clockwise)

Preceding gene: 82544168

Following gene: 82544173

Centisome position: 36.95

GC content: 50.4

Gene sequence:

>990_bases
ATGAAACTCGCCGTTTATAGCACAAAACAGTACGACAAGAAGTACCTGCAACAGGTGAACGAGTCCTTTGGCTTTGAGCT
GGAATTTTTTGACTTTCTGCTGACGGAAAAAACCGCTAAAACTGCCAATGGCTGCGAAGCGGTATGTATTTTCGTAAACG
ATGACGGCAGCCGCCCGGTGCTGGAAGAGCTGAAAAAGCACGGCGTTAAATATATCGCCCTGCGCTGTGCCGGTTTCAAT
AACGTCGACCTTGATGCGGCAAAAGAACTGGGGCTGAAAGTAGTCCGTGTTCCAGCCTATGATCCAGAGGCCGTTGCTGA
ACACGCCATCGGTATGATGATGACGCTGAACCGCCGTATTCACCGCGCGTATCAGCGTACCCGTGACGCTAACTTCTCTC
TGGAAGGTCTGACCGGCTTTACTATGTATGGCAAAACGGCAGGCGTTATCGGTACCGGTAAAATCGGTGTGGCGATGCTG
CGCATTCTGAAAGGTTTTGGTATGCGTCTGCTGGCGTTCGATCCGTATCCAAGTGCAGCGGCGCTGGAACTCGGTGTGGA
GTATGTCGATCTGCCAACCCTGTTCTCTGAATCAGACGTTATCTCTCTGCACTGCCCGCTGACACCGGCAAACTACCATC
TGTTGAACGAAGCCGCCTTCGATCAGATGAAAAATGGCGTGATGATCGTCAATACCAGTCGCGGTGCATTGATTGATTCT
CAGGCAGCAATTGAAGCGCTGAAAAATCAGAAAATTGGTTCGTTGGGTATGGACGTGTATGAGAACGAACGCGATCTGTT
CTTTGAAGATAAATCCAACGACGTGATCCAGGATGACGTATTCCGTCGCCTGTCTGCCTGCCACAACGTGCTGTTTACCG
GGCACCAGGCATTCCTGACAGCAGAAGCTCTGACCAGTATTTCTCAGACTACGCTGCAAAACTTAAGCAATCTGGAAAAA
GGCGAAACCTGCCCGAACGAACTGGTTTAA

Upstream 100 bases:

>100_bases
ATGAATTTTTCAATATCGCCATGGTTTTCAATTATATTTGAAATTTTGTAAAATATTTTTAGTGGCTTAAATGTGATTCA
ACATCACTGGAGAAAGTCTT

Downstream 100 bases:

>100_bases
TGTTGCAGCTCCCCTGCAATCCAGGGGAGCTGATTCAGATAATCCCCAATGACCTTTCATTCTCTATTCTTAAAATAGTC
CTGAGTCAGAAACTGTAATT

Product: fermentative D-lactate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Sequences:

>Translated_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV
>Mature_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=200, Percent_Identity=34, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=32.5396825396825, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=32.5396825396825, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=32.5396825396825, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=32.5396825396825, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI145580575, Length=248, Percent_Identity=33.0645161290323, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=201, Percent_Identity=32.3383084577114, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=99.3920972644377, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=27.1375464684015, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=29.7413793103448, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788660, Length=205, Percent_Identity=26.3414634146341, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=28.7769784172662, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=202, Percent_Identity=26.7326732673267, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=248, Percent_Identity=29.4354838709677, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=28.8194444444444, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=30.6034482758621, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=28.2157676348548, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45552429, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585514, Length=201, Percent_Identity=29.8507462686567, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574282, Length=201, Percent_Identity=29.8507462686567, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574284, Length=201, Percent_Identity=29.8507462686567, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45551003, Length=201, Percent_Identity=29.8507462686567, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574286, Length=238, Percent_Identity=27.3109243697479, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571528, Length=260, Percent_Identity=25.7692307692308, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36463; Mature: 36463

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEAAFDQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEAAFDQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]