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The map label for this gene is nudG [H]

Identifier: 82543838

GI number: 82543838

Start: 1314150

End: 1314557

Strand: Reverse

Name: nudG [H]

Synonym: SBO_1330

Alternate gene names: 82543838

Gene position: 1314557-1314150 (Counterclockwise)

Preceding gene: 82543840

Following gene: 82543836

Centisome position: 29.08

GC content: 53.92

Gene sequence:

>408_bases
ATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGA
TCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCTGGATGAAAGCCAGCAGCAGGCACTGGTGCGTGAGTTAA
ACGAAGAATTGGGCATCGAAGCAACTGTGGGTGAGTATGTTGCCAGCCATCAGCGAGAAGTTTCGGGCCGGATTATCCAT
CTTCATGCCTGGCACGTACCCGACTTCCACGGGACGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGA
AGAGGCGCTGCAATATCCGCTGGCCCCTACTGACATTCCACTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGAGCAG
CGGATTAG

Upstream 100 bases:

>100_bases
TGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCT
TATCTGCCCGTGTGTAATTT

Downstream 100 bases:

>100_bases
TGCTAAGGGTTTTGTCATCCCGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGA
TCCTGATAAACGCAGCACTG

Product: pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPTDIPLLEAFMALRAARAAD

Sequences:

>Translated_135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPTDIPLLEAFMALRAARAAD
>Mature_135_residues
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIEATVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPTDIPLLEAFMALRAARAAD

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=135, Percent_Identity=96.2962962962963, Blast_Score=261, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 14996; Mature: 14996

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEEECCHHHHHHHHHHHHHHCCC
ATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPTDIP
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCH
LLEAFMALRAARAAD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEEECCHHHHHHHHHHHHHHCCC
ATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPTDIP
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCH
LLEAFMALRAARAAD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]