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The map label for this gene is insB [H]

Identifier: 82543699

GI number: 82543699

Start: 1179734

End: 1180165

Strand: Direct

Name: insB [H]

Synonym: SBO_1183

Alternate gene names: 82543699

Gene position: 1179734-1180165 (Clockwise)

Preceding gene: 82543698

Following gene: 82543706

Centisome position: 26.1

GC content: 51.16

Gene sequence:

>432_bases
GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCATGATTGTCTGCGTGAAAATGGACGAACAGTGGGGCTACGTCGG
TGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAAC
GCACTCTGACCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCG
CTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAA
TCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCG
GGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCACCACGGGAATATCTGCGGCA
GCGGGCTTGTAATGGGTTAA

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCGAAATTCTGGTTCTTAGGTTCTATTCCGTGGGAGCGTAAAACCGTCACGCCAGCCAGTTTGTGTG
AAGTGGTCAACAAAAACTGG

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MITDVWKYRGKSMIVCVKMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVVWMTDGWP
LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Sequences:

>Translated_143_residues
MITDVWKYRGKSMIVCVKMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVVWMTDGWP
LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
>Mature_143_residues
MITDVWKYRGKSMIVCVKMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLERLLSLLSAFEVVVWMTDGWP
LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1787223, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1789853, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1788203, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1786203, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1786470, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,
Organism=Escherichia coli, GI1786459, Length=134, Percent_Identity=88.8059701492537, Blast_Score=253, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17138; Mature: 17138

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDVWKYRGKSMIVCVKMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLE
CCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSL
HHHHHHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
SFSKSVELHDKVIGHYLNIKHYQ
CCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MITDVWKYRGKSMIVCVKMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLTTLE
CCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSL
HHHHHHHHHHEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
SFSKSVELHDKVIGHYLNIKHYQ
CCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]