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The map label for this gene is ipaH_2 [H]

Identifier: 82543497

GI number: 82543497

Start: 984407

End: 986050

Strand: Direct

Name: ipaH_2 [H]

Synonym: SBO_0953

Alternate gene names: 82543497

Gene position: 984407-986050 (Clockwise)

Preceding gene: 82543496

Following gene: 82543504

Centisome position: 21.78

GC content: 48.05

Gene sequence:

>1644_bases
ATGCTTCCTGTAAATAATCCCCCCCTATCCACTGGAAACGTCTCTTTTTACAGAACTACATCAATCGACAATGTTCACAA
TAATTATCTCTCCGAATGGGTTGAATGGACTAAAAACAGCATTTCCGGAGAAAACAGGGAAACTGCTTTTACCCGGCTCC
AATTATGTCTGGAAAACAGTGAAACATCGTTGGACTTATCTTGTTTAGGTCTCAGATCTCTACCACGATTGCCTGACAAT
CTTGATGAAATTAATGTAAGCAATAACCAACTATCAATGCTCCCCGAGCTACCAAGGGCATTGAAAGAACTGAATGCAAG
CAGTAATCAATTATCTGCACTTCCTGAATTACCAGTGTCGCTGGAATATATAAATGTGAGTGATAACCATTTGTTCGCAC
TTCCTGAATTACCTGCGTCACTAGAATATATTAACGTAAGTGACAATCACCTGTCTGTACTTCCGAGGTTACCAATGTCA
TTGGAATTACTTGATGCAGCCAGAAATGCTTTGGAAGTAATACCAGATTTTCCAGAAAGAGATGATCATATTATAAGAAT
ATTCTGGCTTAATCAGAACCGGATCACGGCAATTCCGGAAAGCATACTTGGCCTCAGTTCTGATAGCGTTGTCAATCTTA
GAGAAAATCAACTATCTCCCAGAATAATGCAAACTTTGTTACAACAAACCGCCCAACCGGACTACCACGGCCCGCAGATT
TACTTCTCCATGAGTGACGGACAACAGAATACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAA
CAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGCACGCCAACACCTTTTCCGCGTTCCTTGACC
GCCTTTCCGATACCGTCTCTGCACGCAATACCTCCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAAACTCAGTGCC
TCTGCGGAGCTTCGACAGCAGTCTTTCGCTGTTGCTGCTGATGCCACTGAGAGCTGTGAGGACCGTGTCGCGCTCACATG
GAACAATCTCCGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCGATAATGATACCGGCGCTCTGCTCTCCC
TGGGCAGGGAAATGTTCCGCCTCGAAATTCTGGAGGACATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGACGAG
ATAGAAGTCTACCTGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCTCTCCACTGCCGTGAAGGAAATGCGTTTCTA
TGGCGTGTCGGGAGTGACAGCAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGTGAAGAGAATGAATTTACGG
ACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAAGCGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAG
AAATATGAGATGCTGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCAGGTCTGAGCGGTGATGCGGA
TGCGGAGAGGGAAGCCGGTGCACAGGTGATGCGTGAGACTGAACAGCAGATTTACCGTCAGCTGACTGACGAGGTACTGG
CCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCATAA

Upstream 100 bases:

>100_bases
CTTAAAATGAAAACAATAAATTTCAATCCTGAAACATTTTATTTTAGCTTATTATTTCCCAGTATCACATTTTATCAGGA
TATCCTTCTGCGAGGTTATT

Downstream 100 bases:

>100_bases
TCACATCGCATAAACCACAGACCGGACTGACTCCGGAAAAACAGAGGCCCGCCCCCGGGCCTCCCCGGATTCATCCGTTT
CCCTGTTCAGCCTGACAGCA

Product: invasion plasmid antigen

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDN
LDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPQI
YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSA
SAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDE
IEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQ
KYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS

Sequences:

>Translated_547_residues
MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDN
LDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPQI
YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSA
SAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDE
IEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQ
KYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS
>Mature_547_residues
MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDN
LDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPQI
YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSA
SAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDE
IEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQ
KYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Synthesizes a 'Lys-48'-linked ubiquitin chain, which requir

COG id: COG4886

COG function: function code S; Leucine-rich repeat (LRR) protein

Gene ontology:

Cell location: Secreted. Host cytoplasm (By similarity). Note=Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm (By similarity) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 9 LRR (leucine-rich) repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001611 [H]

Pfam domain/function: PF00560 LRR_1 [H]

EC number: NA

Molecular weight: Translated: 62119; Mature: 62119

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENS
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
ETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVS
CCCEEHEECCHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCE
LEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEVIPDFPER
EEEEECCCCEEEECCCCCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCC
DDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPQI
CCCEEEEEEECCCCEEECCHHHHCCCCCCEEECHHHCCCHHHHHHHHHHHCCCCCCCCEE
YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVS
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQAS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHHHHHHHHHHHHC
EGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQ
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPEN
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GSQLHHS
CCCCCCC
>Mature Secondary Structure
MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENS
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
ETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVS
CCCEEHEECCHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCE
LEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEVIPDFPER
EEEEECCCCEEEECCCCCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCC
DDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPQI
CCCEEEEEEECCCCEEECCHHHHCCCCCCEEECHHHCCCHHHHHHHHHHHCCCCCCCCEE
YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVS
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQAS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEHHHHHHHHHHHHHC
EGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWALAEEQ
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPEN
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GSQLHHS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12384590 [H]