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The map label for this gene is miaA

Identifier: 78780113

GI number: 78780113

Start: 1614761

End: 1615660

Strand: Direct

Name: miaA

Synonym: PMT9312_1728

Alternate gene names: 78780113

Gene position: 1614761-1615660 (Clockwise)

Preceding gene: 78780106

Following gene: 78780114

Centisome position: 94.47

GC content: 30.0

Gene sequence:

>900_bases
ATGTCTTCTTATCCCCCTCATGTAATAGTTCTAATTGGAGCCACAGCAAGTGGGAAAACAGAATTAGCTATCGAAATTGC
AGAATATTTTAAAACTCGTATACATAATATCGATTCGAGACAAATTTATAAATCTATGGATATTGGAACAGCCAAACCAT
CTGAGAATCAACAAAAAAAAATAAAGCATTTTCTAATAGATATAGAAGAACCTATTAATCCAATTAATGTAAAACAATTT
CAAGAAATTGCTCAAAAATCAATACAAAAAGAAATTAAGCAAAATAATTTACCTCTACTTGTTGGAGGAAGTGGTTTGTA
TATGAACGCAATAACCAAAGGTTTTTTTGTGCCGGATGTCCCTCCGCAAAATAATTTGAGAGAGCAATTGGAGGAACTTG
GTCAAAAAGAATGTTGGGAGCTTTTAAAAAATTGTGATCCAATTTCGGCAAAAAAAATCAATCTTGCTGATCAAATTAGA
ACAATAAGAGCTTTGGAAGTATTTTATGTAACCGGTAAACCTTTATCTTCTCAAAAAGTTCAAAAACCGCCTCAATGGAA
AATACTAGAGCTTGGATTAAATAGAGATAATTTAAAAGAAAGAATTTCACGAAGAACAAAAAATATGTTTTTATCTGGAA
TTATTGAAGAGACGAAAAATCTTATTTCTCGATATGGATCTGATTTACCAATATTAGAAACTATTGGTTACCGTGAAGCT
AAAGATGTTTTAAATAATAATTTACCGATTGATAAGGCGATTGAGTTAACTAATATAAAAACGAACCAATTTGCCAAAAG
GCAGAAAACATGGTTTCGTAATAAAAATAATCCTATTTGGCTTAATAACAAAAACCTGCTAAAAGATGCAATAATTAAGA
TAGAGTCTTTTTTAGACTAA

Upstream 100 bases:

>100_bases
TTCATTAAACTTACCACCGCAATAAGAGAAAGGCTCGAAGTCTATGAAAAATTTAATCACTTATATTATATAACTAATAA
ATTTTTCTTCAGAAATTCAT

Downstream 100 bases:

>100_bases
CTTTATGAAAAATAGATTTTTGTTCATCATCCAATCAATTTATTAACCAAACTGAATGCCACCACGCCCACGCTTTGACC
GTCGCTCTCCTGTTAGGGAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSSYPPHVIVLIGATASGKTELAIEIAEYFKTRIHNIDSRQIYKSMDIGTAKPSENQQKKIKHFLIDIEEPINPINVKQF
QEIAQKSIQKEIKQNNLPLLVGGSGLYMNAITKGFFVPDVPPQNNLREQLEELGQKECWELLKNCDPISAKKINLADQIR
TIRALEVFYVTGKPLSSQKVQKPPQWKILELGLNRDNLKERISRRTKNMFLSGIIEETKNLISRYGSDLPILETIGYREA
KDVLNNNLPIDKAIELTNIKTNQFAKRQKTWFRNKNNPIWLNNKNLLKDAIIKIESFLD

Sequences:

>Translated_299_residues
MSSYPPHVIVLIGATASGKTELAIEIAEYFKTRIHNIDSRQIYKSMDIGTAKPSENQQKKIKHFLIDIEEPINPINVKQF
QEIAQKSIQKEIKQNNLPLLVGGSGLYMNAITKGFFVPDVPPQNNLREQLEELGQKECWELLKNCDPISAKKINLADQIR
TIRALEVFYVTGKPLSSQKVQKPPQWKILELGLNRDNLKERISRRTKNMFLSGIIEETKNLISRYGSDLPILETIGYREA
KDVLNNNLPIDKAIELTNIKTNQFAKRQKTWFRNKNNPIWLNNKNLLKDAIIKIESFLD
>Mature_298_residues
SSYPPHVIVLIGATASGKTELAIEIAEYFKTRIHNIDSRQIYKSMDIGTAKPSENQQKKIKHFLIDIEEPINPINVKQFQ
EIAQKSIQKEIKQNNLPLLVGGSGLYMNAITKGFFVPDVPPQNNLREQLEELGQKECWELLKNCDPISAKKINLADQIRT
IRALEVFYVTGKPLSSQKVQKPPQWKILELGLNRDNLKERISRRTKNMFLSGIIEETKNLISRYGSDLPILETIGYREAK
DVLNNNLPIDKAIELTNIKTNQFAKRQKTWFRNKNNPIWLNNKNLLKDAIIKIESFLD

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=25.08038585209, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1790613, Length=291, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=26.2237762237762, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24649830, Length=262, Percent_Identity=29.7709923664122, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROM9 (Q318A6)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_398225.1
- ProteinModelPortal:   Q318A6
- SMR:   Q318A6
- STRING:   Q318A6
- GeneID:   3766553
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1728
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   PMAR74546:PMT9312_1728-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34363; Mature: 34232

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYPPHVIVLIGATASGKTELAIEIAEYFKTRIHNIDSRQIYKSMDIGTAKPSENQQKK
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHH
IKHFLIDIEEPINPINVKQFQEIAQKSIQKEIKQNNLPLLVGGSGLYMNAITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCC
PPQNNLREQLEELGQKECWELLKNCDPISAKKINLADQIRTIRALEVFYVTGKPLSSQKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHC
QKPPQWKILELGLNRDNLKERISRRTKNMFLSGIIEETKNLISRYGSDLPILETIGYREA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHH
KDVLNNNLPIDKAIELTNIKTNQFAKRQKTWFRNKNNPIWLNNKNLLKDAIIKIESFLD
HHHHCCCCCHHHCEEECCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSYPPHVIVLIGATASGKTELAIEIAEYFKTRIHNIDSRQIYKSMDIGTAKPSENQQKK
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHH
IKHFLIDIEEPINPINVKQFQEIAQKSIQKEIKQNNLPLLVGGSGLYMNAITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCC
PPQNNLREQLEELGQKECWELLKNCDPISAKKINLADQIRTIRALEVFYVTGKPLSSQKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHC
QKPPQWKILELGLNRDNLKERISRRTKNMFLSGIIEETKNLISRYGSDLPILETIGYREA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHH
KDVLNNNLPIDKAIELTNIKTNQFAKRQKTWFRNKNNPIWLNNKNLLKDAIIKIESFLD
HHHHCCCCCHHHCEEECCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA