Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 78780096

GI number: 78780096

Start: 1599215

End: 1600753

Strand: Reverse

Name: lysS

Synonym: PMT9312_1711

Alternate gene names: 78780096

Gene position: 1600753-1599215 (Counterclockwise)

Preceding gene: 78780097

Following gene: 78780095

Centisome position: 93.65

GC content: 31.77

Gene sequence:

>1539_bases
TTGTCTGAAATAAGAGAAGCACGCTTACAAAAAGCTAATTCACTAGTTAGTAAAGGATTTGCTTCTTACGCAGAGAGTTT
TAGCGTTTCACATACTACAAAATTTCTTATTCAAAAATTTGATTATTTAGAAAATGGTCAAGAGGAAGACTTCAGTGTTT
CTCTCGCTGGTAGAGTGATGGCAAAAAGGGTAATGGGTAAAATTGCTTTTTTCACAATAAGCGATCAAGAAGGACAGATT
CAGCTTTATCTAGATAAAAGGATGATTAATTGCAATTTAGAAAACCAAAAATTACTTTCTTTTGAAGATATTAAGGAAAT
AGTAGATATTGGTGATTGGATAGGCGTATACGGCACTATTAAAAAGACTAATAAAGGGGAGCTTTCAATTAAAGTAGAAA
AATGGGAAATGTTATCCAAATCATTACAGCCTTTACCCGATAAATGGCATGGATTGACTGATATTGAAAAAAGATATAGA
CAACGTTATCTAGATTTAATAGTTAATCCACACTCTAAAAATGTATTTAAAACAAGAGCAAAATGTATAAGTTTTATAAG
ACAATGGCTAGATAATAGAAATTTTTTAGAGATAGAGACTCCAATTCTTCAATCTGAAGCTGGTGGTGCTGAAGCAAGAC
CATTTATTACTCATCACAATACATTGGATATTCCGCTGTATCTAAGAATAGCTACAGAATTACATTTAAAAAGAATGGTT
GTTGGAGGATTTGAGAAAGTTTATGAATTGGGAAGAATCTTCCGTAATGAGGGGATAAGTACAAAGCATAATCCAGAATT
TACCTCAGTTGAAATTTATCAAGCTTTTTCTAACTATGTCGATATGATGAATCTAACAGAAGAACTGATTAAAGATATTG
TATTTAATGTGTGTGGTTCTTTAGTTATAAATTATCAAAATAAGGAAATTGATTTTTCTAAACCTTGGTCAAGAATATCC
ATGAAAGATATTGTCAAAAAATATACAGGGATTGATTTTGATTCTTTCAGTGGAGACTTTCAATCAGCAAAACAAGCCGT
TAAAAGTATAAATGTTGAATTTTCTAATAAAGTGAATTCTATGGGAAGACTTTTAAATGAGGTCTTCGAGCAAAAAGTTG
AGTCAGAACTTATAGAACCTACTTTTGTTATCGATTATCCTGTTGAAATTTCTCCTTTAGCTAGGCCTCATCTTGATAAT
AAAGAAATGGTTCAGAGATTCGAATTATTTATTGTTGGACGAGAGCTAGCAAATGCGTTTAGTGAGTTGATAGATCCAGT
AGATCAAAGAGAAAGAATGCAATTACAGCAATCTCTTAGAGATGAAGGAGATCATGAGGCTCATTGTATAGATGAAGATT
TTTTGAATGCTTTGGAGATTGGTATGCCTCCTACTGGGGGATTAGGCATAGGTATTGATAGACTGATTATGCTAATTACT
AATAGTCCATCAATTAGAGATGTAATCCCTTTCCCATTGTTAAAACCAGAAATAACTTCCAATAAAAACGAAAAATTACC
CTTGAATGAAGTAAAATAA

Upstream 100 bases:

>100_bases
GTTATGCGTCTCAGAGAGTTGGCGAAACAGCAACATCTTTGGCAAGTTGAGCCATAGTCTGATAATTTTATTAAATAAAA
CAAATATATTAAAATAAATT

Downstream 100 bases:

>100_bases
GATAATTAATCCAATACGCAATTTTTTAAATGAGTGGTGAACGTGTTGGTTTCCGTTTCAAACACGCGGATGCAGTAGTA
AAAAGAAATCCTCAAGGCCG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSEIREARLQKANSLVSKGFASYAESFSVSHTTKFLIQKFDYLENGQEEDFSVSLAGRVMAKRVMGKIAFFTISDQEGQI
QLYLDKRMINCNLENQKLLSFEDIKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYR
QRYLDLIVNPHSKNVFKTRAKCISFIRQWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
VGGFEKVYELGRIFRNEGISTKHNPEFTSVEIYQAFSNYVDMMNLTEELIKDIVFNVCGSLVINYQNKEIDFSKPWSRIS
MKDIVKKYTGIDFDSFSGDFQSAKQAVKSINVEFSNKVNSMGRLLNEVFEQKVESELIEPTFVIDYPVEISPLARPHLDN
KEMVQRFELFIVGRELANAFSELIDPVDQRERMQLQQSLRDEGDHEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLIT
NSPSIRDVIPFPLLKPEITSNKNEKLPLNEVK

Sequences:

>Translated_512_residues
MSEIREARLQKANSLVSKGFASYAESFSVSHTTKFLIQKFDYLENGQEEDFSVSLAGRVMAKRVMGKIAFFTISDQEGQI
QLYLDKRMINCNLENQKLLSFEDIKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYR
QRYLDLIVNPHSKNVFKTRAKCISFIRQWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
VGGFEKVYELGRIFRNEGISTKHNPEFTSVEIYQAFSNYVDMMNLTEELIKDIVFNVCGSLVINYQNKEIDFSKPWSRIS
MKDIVKKYTGIDFDSFSGDFQSAKQAVKSINVEFSNKVNSMGRLLNEVFEQKVESELIEPTFVIDYPVEISPLARPHLDN
KEMVQRFELFIVGRELANAFSELIDPVDQRERMQLQQSLRDEGDHEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLIT
NSPSIRDVIPFPLLKPEITSNKNEKLPLNEVK
>Mature_511_residues
SEIREARLQKANSLVSKGFASYAESFSVSHTTKFLIQKFDYLENGQEEDFSVSLAGRVMAKRVMGKIAFFTISDQEGQIQ
LYLDKRMINCNLENQKLLSFEDIKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQ
RYLDLIVNPHSKNVFKTRAKCISFIRQWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVV
GGFEKVYELGRIFRNEGISTKHNPEFTSVEIYQAFSNYVDMMNLTEELIKDIVFNVCGSLVINYQNKEIDFSKPWSRISM
KDIVKKYTGIDFDSFSGDFQSAKQAVKSINVEFSNKVNSMGRLLNEVFEQKVESELIEPTFVIDYPVEISPLARPHLDNK
EMVQRFELFIVGRELANAFSELIDPVDQRERMQLQQSLRDEGDHEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITN
SPSIRDVIPFPLLKPEITSNKNEKLPLNEVK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=503, Percent_Identity=39.9602385685885, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI194272210, Length=503, Percent_Identity=39.9602385685885, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI45439306, Length=482, Percent_Identity=22.1991701244813, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI226491502, Length=335, Percent_Identity=25.3731343283582, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789256, Length=497, Percent_Identity=47.0824949698189, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790571, Length=496, Percent_Identity=47.1774193548387, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=28.5276073619632, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788173, Length=322, Percent_Identity=24.8447204968944, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787161, Length=254, Percent_Identity=25.9842519685039, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535927, Length=509, Percent_Identity=40.0785854616896, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535925, Length=509, Percent_Identity=40.0785854616896, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=509, Percent_Identity=40.0785854616896, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17551876, Length=486, Percent_Identity=21.8106995884774, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=289, Percent_Identity=24.2214532871972, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=496, Percent_Identity=41.1290322580645, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324256, Length=479, Percent_Identity=30.8977035490605, Blast_Score=207, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6323011, Length=484, Percent_Identity=22.7272727272727, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=306, Percent_Identity=24.5098039215686, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=510, Percent_Identity=40.3921568627451, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=40.3921568627451, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24584738, Length=308, Percent_Identity=24.6753246753247, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58925; Mature: 58794

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIREARLQKANSLVSKGFASYAESFSVSHTTKFLIQKFDYLENGQEEDFSVSLAGRVM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
AKRVMGKIAFFTISDQEGQIQLYLDKRMINCNLENQKLLSFEDIKEIVDIGDWIGVYGTI
HHHHHHHEEEEEEECCCCEEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCEEE
KKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNVFKTRA
EECCCCCEEEEEHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KCISFIRQWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
HHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHH
VGGFEKVYELGRIFRNEGISTKHNPEFTSVEIYQAFSNYVDMMNLTEELIKDIVFNVCGS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVINYQNKEIDFSKPWSRISMKDIVKKYTGIDFDSFSGDFQSAKQAVKSINVEFSNKVNS
HEEECCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
MGRLLNEVFEQKVESELIEPTFVIDYPVEISPLARPHLDNKEMVQRFELFIVGRELANAF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SELIDPVDQRERMQLQQSLRDEGDHEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLIT
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHEEEEE
NSPSIRDVIPFPLLKPEITSNKNEKLPLNEVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEIREARLQKANSLVSKGFASYAESFSVSHTTKFLIQKFDYLENGQEEDFSVSLAGRVM
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
AKRVMGKIAFFTISDQEGQIQLYLDKRMINCNLENQKLLSFEDIKEIVDIGDWIGVYGTI
HHHHHHHEEEEEEECCCCEEEEEEECEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCEEE
KKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNVFKTRA
EECCCCCEEEEEHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KCISFIRQWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMV
HHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHH
VGGFEKVYELGRIFRNEGISTKHNPEFTSVEIYQAFSNYVDMMNLTEELIKDIVFNVCGS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVINYQNKEIDFSKPWSRISMKDIVKKYTGIDFDSFSGDFQSAKQAVKSINVEFSNKVNS
HEEECCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
MGRLLNEVFEQKVESELIEPTFVIDYPVEISPLARPHLDNKEMVQRFELFIVGRELANAF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SELIDPVDQRERMQLQQSLRDEGDHEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLIT
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHEEEEE
NSPSIRDVIPFPLLKPEITSNKNEKLPLNEVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA