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The map label for this gene is polA [H]

Identifier: 78780061

GI number: 78780061

Start: 1565795

End: 1566430

Strand: Direct

Name: polA [H]

Synonym: PMT9312_1676

Alternate gene names: 78780061

Gene position: 1565795-1566430 (Clockwise)

Preceding gene: 78780059

Following gene: 78780067

Centisome position: 91.61

GC content: 27.67

Gene sequence:

>636_bases
ATGACTATTGAAAATAAAAATATTGATTTTCTTTATAGTGATTTAACAGCAGATTTATATAATATCTATAAAAAATCATC
CTACCTTGCTATTGACACTGAAGCAATGGGGTTGATTCATGGAAGAGATAGATTATGTTTAGTACAAATATGCAATGAAC
TTAGAAGAACAGCCTGTATAAAAATCGAACTTAATAGATCTTCTGCTCCCAATTTAAAAGCACTTCTTGAAGATGAAAAA
ATTACTAAAATCTTTCACTATGCAAGATTTGATGTAGCAGCGCTAAAATGCAATCTTGGAATTAATACAAAAAATATTTT
TTGTACAAAAATTGCAAGTAAGTTGGCAAGAACATATACAAATAAACACGGTTTAAAAGATTTAATTAATGAATTGTTAG
GGGTAGAATTAGACAAAAGTTCACAAAGCAGTGATTGGGGTAGCAAAGAAGATTTAACAAAAGATCAATTAGATTATGCT
TCAAATGATGTTAGATATTTAATTGAAGCGATGCATAAATTAAAAGTCATCTTAGATAGAGAGAATAGATATGAGTTAGC
TCAAAAATGTTTCAAAACAGTTTCAGTTCATGCTGATTTAGACATCCTAAAATTTTCAAATATATTTGAACATTAA

Upstream 100 bases:

>100_bases
ATTTATAGATTTATTCTGTAAAATAAGTGCACCAGATAAAAAAATCTTAATTACATAAAAACTACGTAATATCGTAAATG
TCTTTAAATACTTATTAAAT

Downstream 100 bases:

>100_bases
AAATCAATCTTCAGTTAAAAAATTATCTTCACCATCAAGAGTTTCCATAAGCTTATCAAGTATTCTTGAAGAACTTAATT
TATTGACTTCGTTTTTTTTA

Product: putative ribonuclease D

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTIENKNIDFLYSDLTADLYNIYKKSSYLAIDTEAMGLIHGRDRLCLVQICNELRRTACIKIELNRSSAPNLKALLEDEK
ITKIFHYARFDVAALKCNLGINTKNIFCTKIASKLARTYTNKHGLKDLINELLGVELDKSSQSSDWGSKEDLTKDQLDYA
SNDVRYLIEAMHKLKVILDRENRYELAQKCFKTVSVHADLDILKFSNIFEH

Sequences:

>Translated_211_residues
MTIENKNIDFLYSDLTADLYNIYKKSSYLAIDTEAMGLIHGRDRLCLVQICNELRRTACIKIELNRSSAPNLKALLEDEK
ITKIFHYARFDVAALKCNLGINTKNIFCTKIASKLARTYTNKHGLKDLINELLGVELDKSSQSSDWGSKEDLTKDQLDYA
SNDVRYLIEAMHKLKVILDRENRYELAQKCFKTVSVHADLDILKFSNIFEH
>Mature_210_residues
TIENKNIDFLYSDLTADLYNIYKKSSYLAIDTEAMGLIHGRDRLCLVQICNELRRTACIKIELNRSSAPNLKALLEDEKI
TKIFHYARFDVAALKCNLGINTKNIFCTKIASKLARTYTNKHGLKDLINELLGVELDKSSQSSDWGSKEDLTKDQLDYAS
NDVRYLIEAMHKLKVILDRENRYELAQKCFKTVSVHADLDILKFSNIFEH

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 24273; Mature: 24142

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIENKNIDFLYSDLTADLYNIYKKSSYLAIDTEAMGLIHGRDRLCLVQICNELRRTACI
CCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEHHHHHEEECCCCCHHHHHHHHHHHHEEE
KIELNRSSAPNLKALLEDEKITKIFHYARFDVAALKCNLGINTKNIFCTKIASKLARTYT
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHH
NKHGLKDLINELLGVELDKSSQSSDWGSKEDLTKDQLDYASNDVRYLIEAMHKLKVILDR
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
ENRYELAQKCFKTVSVHADLDILKFSNIFEH
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIENKNIDFLYSDLTADLYNIYKKSSYLAIDTEAMGLIHGRDRLCLVQICNELRRTACI
CCCCCCCCEEHHHHHHHHHHHHHCCCEEEEEHHHHHEEECCCCCHHHHHHHHHHHHEEE
KIELNRSSAPNLKALLEDEKITKIFHYARFDVAALKCNLGINTKNIFCTKIASKLARTYT
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHH
NKHGLKDLINELLGVELDKSSQSSDWGSKEDLTKDQLDYASNDVRYLIEAMHKLKVILDR
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
ENRYELAQKCFKTVSVHADLDILKFSNIFEH
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]