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The map label for this gene is folC [H]

Identifier: 78779782

GI number: 78779782

Start: 1313638

End: 1314870

Strand: Direct

Name: folC [H]

Synonym: PMT9312_1398

Alternate gene names: 78779782

Gene position: 1313638-1314870 (Clockwise)

Preceding gene: 78779781

Following gene: 78779785

Centisome position: 76.86

GC content: 29.6

Gene sequence:

>1233_bases
TTGAAAAATGCAAATTTAAAAAATTTTAAATTATTATCACCCAAATATGAAAGGGATAATATCAAGTTAGGTTTATCTAG
AATTAAAAAAGCACTTCAGAAACTTGGTAATCCTTGCAAGAATATCCCAGCAATACAAATTATTGGAACTAATGGGAAAG
GATCAATCGCGGCATATCTAGAAAGTATACTTTTTGAAGCTAAAATGAATTTTGGGGTAACTACATCTCCTCATCTTTTT
GATATATGTGAGAGGATTAGAGTTAATAAAAAAAATATTAACAAAACTGATTTTGAAATAATCTATAGATTAATAGAAAA
AAATTTTTCAACATATGAATTAACTCCTTTTGAAAAAATTATTTGCTGCGCACTAAATTTTTTTGACAATGAAAAAGTTG
AATTAATAATTCTTGAAGCTGGCTTAGGAGGACGATTAGACGCGACAACAGCCCATAAATTAAGACCAATCATTGCTATT
GGAAACATTGGCTTAGACCATAAAGAATTTCTTGGAGATACGATTGAAAAAATTGCCAAAGAAAAATTAGCAGTTATTGA
AAAAAACTCAATTGTTATTTCCTGCAACCAAAACAGTCAAGTTGAAAATTTAATAACCAAAAAAGTTGAAGAGGTTGGAG
CAGAAATCATCTGGAAAGATTCAACTTCAAACAGCTATGAGCTTGGATTAAAAGGAATTTTCCAAAAGCAAAATGCTTCA
GTCGCCATTGGAGTAATTGAAGCTCTTAATAAGTTGGGATTTAGTATTAAAGAAAAATATATATCTGAAGGTCTTAAAAA
AACAACTTGGCAAGGAAGGTTAGAAATAATAACTTACTTAAACAAAAAAATTCTTGTAGATTGTGCGCATAATTATCCTG
CTGCAAAAGCACTTTCTAATGAGCGAAGCAATTGGAAGCATGAAGAAGAAGGAATTTATTGGATTTTAGGTGTCCAAAGA
CAAAAAGATGTGCACGCAATATTAAAAACACTTCTAAAGAAAAATGATCACTTATTACTTGTTCCAGTTCCAAACCAACC
TAGTTGGCAATTAAAAGAACTTTCTCAGATTAAAGAAATTGATTTTCAAAACATAATTGAGTTTGAGAAATTTGAATTTG
CCATTGAATATTTATTTGCCCTTCAAAAATGGCCCCTTAATCTTCCTGTTTTAACAGGTTCTATTTTTTTAGTGGCTGAA
TTTATTAAATTTGCCAATAAACAGAAATGTTAA

Upstream 100 bases:

>100_bases
TTGTTAGGATTGTCCCACCATTAATTATATCTCGAAGAGAAATTAATATTCTTTTAGATAAGCTGAATTCAATTTTTGAG
GAGTTATAGCACTTTAAATT

Downstream 100 bases:

>100_bases
ATATTAAATTGTTCCTTTATTTTCCAACCCTCTAATTTCCCTGGATTTAATAGCCCTTTAGGATCAAATTTTAATTTCGC
TTTTACTTGATCAGAGTCCA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKNANLKNFKLLSPKYERDNIKLGLSRIKKALQKLGNPCKNIPAIQIIGTNGKGSIAAYLESILFEAKMNFGVTTSPHLF
DICERIRVNKKNINKTDFEIIYRLIEKNFSTYELTPFEKIICCALNFFDNEKVELIILEAGLGGRLDATTAHKLRPIIAI
GNIGLDHKEFLGDTIEKIAKEKLAVIEKNSIVISCNQNSQVENLITKKVEEVGAEIIWKDSTSNSYELGLKGIFQKQNAS
VAIGVIEALNKLGFSIKEKYISEGLKKTTWQGRLEIITYLNKKILVDCAHNYPAAKALSNERSNWKHEEEGIYWILGVQR
QKDVHAILKTLLKKNDHLLLVPVPNQPSWQLKELSQIKEIDFQNIIEFEKFEFAIEYLFALQKWPLNLPVLTGSIFLVAE
FIKFANKQKC

Sequences:

>Translated_410_residues
MKNANLKNFKLLSPKYERDNIKLGLSRIKKALQKLGNPCKNIPAIQIIGTNGKGSIAAYLESILFEAKMNFGVTTSPHLF
DICERIRVNKKNINKTDFEIIYRLIEKNFSTYELTPFEKIICCALNFFDNEKVELIILEAGLGGRLDATTAHKLRPIIAI
GNIGLDHKEFLGDTIEKIAKEKLAVIEKNSIVISCNQNSQVENLITKKVEEVGAEIIWKDSTSNSYELGLKGIFQKQNAS
VAIGVIEALNKLGFSIKEKYISEGLKKTTWQGRLEIITYLNKKILVDCAHNYPAAKALSNERSNWKHEEEGIYWILGVQR
QKDVHAILKTLLKKNDHLLLVPVPNQPSWQLKELSQIKEIDFQNIIEFEKFEFAIEYLFALQKWPLNLPVLTGSIFLVAE
FIKFANKQKC
>Mature_410_residues
MKNANLKNFKLLSPKYERDNIKLGLSRIKKALQKLGNPCKNIPAIQIIGTNGKGSIAAYLESILFEAKMNFGVTTSPHLF
DICERIRVNKKNINKTDFEIIYRLIEKNFSTYELTPFEKIICCALNFFDNEKVELIILEAGLGGRLDATTAHKLRPIIAI
GNIGLDHKEFLGDTIEKIAKEKLAVIEKNSIVISCNQNSQVENLITKKVEEVGAEIIWKDSTSNSYELGLKGIFQKQNAS
VAIGVIEALNKLGFSIKEKYISEGLKKTTWQGRLEIITYLNKKILVDCAHNYPAAKALSNERSNWKHEEEGIYWILGVQR
QKDVHAILKTLLKKNDHLLLVPVPNQPSWQLKELSQIKEIDFQNIIEFEKFEFAIEYLFALQKWPLNLPVLTGSIFLVAE
FIKFANKQKC

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=289, Percent_Identity=25.9515570934256, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI66932990, Length=289, Percent_Identity=25.9515570934256, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788654, Length=347, Percent_Identity=28.5302593659942, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=323, Percent_Identity=25.077399380805, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71984923, Length=323, Percent_Identity=25.077399380805, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=323, Percent_Identity=25.077399380805, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=431, Percent_Identity=30.3944315545244, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=320, Percent_Identity=28.4375, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=279, Percent_Identity=26.1648745519713, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641571, Length=297, Percent_Identity=25.5892255892256, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=30.1675977653631, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46679; Mature: 46679

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNANLKNFKLLSPKYERDNIKLGLSRIKKALQKLGNPCKNIPAIQIIGTNGKGSIAAYL
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ESILFEAKMNFGVTTSPHLFDICERIRVNKKNINKTDFEIIYRLIEKNFSTYELTPFEKI
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCHHHHH
ICCALNFFDNEKVELIILEAGLGGRLDATTAHKLRPIIAIGNIGLDHKEFLGDTIEKIAK
HHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
EKLAVIEKNSIVISCNQNSQVENLITKKVEEVGAEIIWKDSTSNSYELGLKGIFQKQNAS
HHHHEEECCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHCHHHHHHCCCCC
VAIGVIEALNKLGFSIKEKYISEGLKKTTWQGRLEIITYLNKKILVDCAHNYPAAKALSN
EEHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHC
ERSNWKHEEEGIYWILGVQRQKDVHAILKTLLKKNDHLLLVPVPNQPSWQLKELSQIKEI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHC
DFQNIIEFEKFEFAIEYLFALQKWPLNLPVLTGSIFLVAEFIKFANKQKC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNANLKNFKLLSPKYERDNIKLGLSRIKKALQKLGNPCKNIPAIQIIGTNGKGSIAAYL
CCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHH
ESILFEAKMNFGVTTSPHLFDICERIRVNKKNINKTDFEIIYRLIEKNFSTYELTPFEKI
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEECCHHHHH
ICCALNFFDNEKVELIILEAGLGGRLDATTAHKLRPIIAIGNIGLDHKEFLGDTIEKIAK
HHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHCCEEEEECCCCCCHHHHHHHHHHHHHH
EKLAVIEKNSIVISCNQNSQVENLITKKVEEVGAEIIWKDSTSNSYELGLKGIFQKQNAS
HHHHEEECCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHCHHHHHHCCCCC
VAIGVIEALNKLGFSIKEKYISEGLKKTTWQGRLEIITYLNKKILVDCAHNYPAAKALSN
EEHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHC
ERSNWKHEEEGIYWILGVQRQKDVHAILKTLLKKNDHLLLVPVPNQPSWQLKELSQIKEI
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHC
DFQNIIEFEKFEFAIEYLFALQKWPLNLPVLTGSIFLVAEFIKFANKQKC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]