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The map label for this gene is murE

Identifier: 78778793

GI number: 78778793

Start: 387107

End: 388642

Strand: Direct

Name: murE

Synonym: PMT9312_0408

Alternate gene names: 78778793

Gene position: 387107-388642 (Clockwise)

Preceding gene: 78778792

Following gene: 78778794

Centisome position: 22.65

GC content: 29.75

Gene sequence:

>1536_bases
ATGAGATCTATAAAATTACATAAACTTTTAGATTTGGTAGGAATTATTCCTTCATTAGATTTAATCGATCATGAAATAAA
TAATATTTCTTTTAACTCTAAAGAAGTACAAAAAGGAACTTTATTTTTAGGTATGCCTGGTTTAAATGTTGATGGAGGAA
AATATTGTATTGAGGCAATTGAAAATGGTGCAGAGGCTGCCATTATTGGGTCTGCTGCAAAAGAGAAAATTGGATCTATT
GATCGAGAAAGGATTTTGGTTATAGAGGATAATTTAGATTATATTTTTGGTCAAATAGTCGCTGAGTTTTGGAATAGGCC
TTCAAGAAAACTTAAACTTATTGGTGTTACGGGTACAAATGGAAAAACGACAATTACTTTTTTATTGGAATATCTTTTAA
AAAAATTAGGGAAAAAGACTGCATTATTTGGGACCTTGCTCAATAGATGGCCTGGCTTTTCAGAAGTGGCTCTTCATACA
ACTGATTTCGCCGATAAACTCCAAAAGAAATTAAATGCTGCTGTTGAGGCAGAATCTGAATTCGCGATATTAGAGGTAAG
TTCTCATTCTATTGCTCAAAACAGGATATCAGGATGCGAATTTGAGGCGGCTATTTTTACTAATTTAACTCAAGATCATC
TTGATTATCACTCAGATATGGAATCATATTTTCAAACAAAAAGAAAATTATTTTTCCCACCTTACTTAAATGAAAAAGAT
GGCATTTGTGTATTAAATCATGATGACCATTGGATATCTAAATTATCATCTGATCTTGAAAAAAGATCTTTATTAGTCTC
TACAAAGATTACTGAAAGTGAATTTGAAAGTGATGATTTTTTTTTCGTAACAGATAAGAAATTTACTGAAATTGGTTCCA
CCTGTATTTTTCATACACCTAGGGAAAAAATTCAACTTTTTGTTCCACTTGTCGGTGAATTTAATTTAATGAATGCGATT
CAAGCAATAACAATTTTGTATAAACTTAATTTTTCTTTAAAAGATCTATCAAAATTAATACAATCTTTCCCTGGTGCTCC
TGGGAGAATGGAGAAAATACAAATTGATAATAATGACGTTTCAAGATCTCTTCCAACAGTAATTGTTGATTATGCCCACA
CTCCTGATGGATTAAAAAAAGTTTTGCAATCAATTAAAAAACTTTGTGAAGGGAAACTCATAACTGTTTTTGGCTGTGGC
GGAGATCGTGATCGTAGTAAAAGGCCTTTAATGGGATCAATAGCTGAAGAGTTTTCTGATCAACTTTTTATAACTTCAGA
TAATCCAAGATCAGAAGAACCCCAAAAGATAGTAAATGATATTTTGATGGGCATAAAAAAAAGAGAAAAAATAACAATTG
AAATTGATAGATTTAAAGCAATAAATGAATCTATTAAATTTGCTAAAAAAAAAGATATTGTTTTAATTGCAGGAAAAGGA
CATGAAGACTACCAAATTCTCAATGATAAAGTTATTAATTTTGATGATAGAAAAATAGCTTATAAATTATTAAAAGAAAA
AAATAAATCTCAATAA

Upstream 100 bases:

>100_bases
TATAGAATTGCCTCGCATTTCTCCAAGATTAAAAGATGATCAATTAAAGAATTGGTTTCAAAAAAATATTAGTACCATCC
TGGAGAAATAATTTTTTTAT

Downstream 100 bases:

>100_bases
AATTTCCTTATCAAATAAGAAAGATCTTTACGCAAATCACAAGAAAATTTTCTTAGAATCACTGGAGTTATTTTTAATAA
AATGAAAGGCTTAGCCTTAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRSIKLHKLLDLVGIIPSLDLIDHEINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKEKIGSI
DRERILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLLNRWPGFSEVALHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQTKRKLFFPPYLNEKD
GICVLNHDDHWISKLSSDLEKRSLLVSTKITESEFESDDFFFVTDKKFTEIGSTCIFHTPREKIQLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIQIDNNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEEFSDQLFITSDNPRSEEPQKIVNDILMGIKKREKITIEIDRFKAINESIKFAKKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ

Sequences:

>Translated_511_residues
MRSIKLHKLLDLVGIIPSLDLIDHEINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKEKIGSI
DRERILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLLNRWPGFSEVALHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQTKRKLFFPPYLNEKD
GICVLNHDDHWISKLSSDLEKRSLLVSTKITESEFESDDFFFVTDKKFTEIGSTCIFHTPREKIQLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIQIDNNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEEFSDQLFITSDNPRSEEPQKIVNDILMGIKKREKITIEIDRFKAINESIKFAKKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
>Mature_511_residues
MRSIKLHKLLDLVGIIPSLDLIDHEINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKEKIGSI
DRERILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALFGTLLNRWPGFSEVALHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQTKRKLFFPPYLNEKD
GICVLNHDDHWISKLSSDLEKRSLLVSTKITESEFESDDFFFVTDKKFTEIGSTCIFHTPREKIQLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIQIDNNDVSRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCG
GDRDRSKRPLMGSIAEEFSDQLFITSDNPRSEEPQKIVNDILMGIKKREKITIEIDRFKAINESIKFAKKKDIVLIAGKG
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=492, Percent_Identity=37.1951219512195, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1786274, Length=367, Percent_Identity=22.8882833787466, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57794; Mature: 57794

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIKLHKLLDLVGIIPSLDLIDHEINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAI
CCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGSAAKEKIGSIDRERILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALFGTLLNRWPGFSEVALHTTDFADKLQKKLNAAVEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQTKRKLFFPPYLNEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCEECCCCCCCCC
GICVLNHDDHWISKLSSDLEKRSLLVSTKITESEFESDDFFFVTDKKFTEIGSTCIFHTP
CEEEECCCHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCEEEEECCHHHHHCCEEEEECC
REKIQLFVPLVGEFNLMNAIQAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIQIDNNDV
HHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCGGDRDRSKRPLMGSIAEEFSD
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
QLFITSDNPRSEEPQKIVNDILMGIKKREKITIEIDRFKAINESIKFAKKKDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEECCC
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
CCCHHHHCCCEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSIKLHKLLDLVGIIPSLDLIDHEINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAI
CCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGSAAKEKIGSIDRERILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALFGTLLNRWPGFSEVALHTTDFADKLQKKLNAAVEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQTKRKLFFPPYLNEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCEECCCCCCCCC
GICVLNHDDHWISKLSSDLEKRSLLVSTKITESEFESDDFFFVTDKKFTEIGSTCIFHTP
CEEEECCCHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCEEEEECCHHHHHCCEEEEECC
REKIQLFVPLVGEFNLMNAIQAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIQIDNNDV
HHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SRSLPTVIVDYAHTPDGLKKVLQSIKKLCEGKLITVFGCGGDRDRSKRPLMGSIAEEFSD
HHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
QLFITSDNPRSEEPQKIVNDILMGIKKREKITIEIDRFKAINESIKFAKKKDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEECCC
HEDYQILNDKVINFDDRKIAYKLLKEKNKSQ
CCCHHHHCCCEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]